Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics

Hough, Kenneth P.; Wilson, Landon; Trevor, Jennifer; Strenkowski, John G.; Maina, Njeri; Kim, Young Il; Spell, Marion; Wang, Yong; Chanda, Diptiman; Dager, Jose Rodriguez; Sharma, Nirmal; Curtiss, Miranda L.; Antony, Veena B.; Dransfield, Mark T.; Chaplin, David; Steele, Chad; Barnes, Stephen; Duncan, Steven R.; Prasain, Jeevan K.; Thannickal, Victor J.; Deshane, Jessy

doi:10.1038/s41598-018-28655-9

Cited by 97 publications

(104 citation statements)

References 112 publications

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“…According to the hypothesis, enriched KOs indicate that POF patients harboured an impaired inflammation condition. Phosphatidylglycerol, phosphate, L-methionine and pyruvate, whose metabolic enzymes were enriched in the POF group, are known to be related to inflammation and Class II antigen [43,44]. In turn, inflammation promotes the production of acetyl-CoA.…”

Section: Discussionmentioning

confidence: 99%

Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

BMC Microbiol

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Background: Primary ovarian failure (POF) is defined as follicular failure in women of reproductive age. Although many factors are speculated to contribute to the occurrence of POF, the exact aetiology remains unclear. Moreover, alterations in the microbiome of patients with POF are poorly studied. Results: This study investigated the vaginal microbiota of 22 patients with POF and 29 healthy individuals. Highthroughput Illumina MiSeq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene was used to evaluate the relationships between the vaginal flora and clinical characteristics of POF. Different from results of previous studies, we found that the diversity and richness of the vaginal flora of patients with POF was significantly different from those of healthy controls. Comparison of the vaginal flora of patients with POF with that of menopausal women revealed that the relative abundance of Lactobacillus was significantly reduced in the latter. A reduced abundance of Lactobacillus was furthermore associated with a lower pregnancy success rate. Of particular interest is that L. gallinarum especially appeared to be beneficially associated with reproductive-related indicators (FSH, E2, AMH, PRL) whilst L. iners appeared to have a detrimental effect. The result of the present study may enable the identification of microbiota associated with POF, however, further investigations of differences in the microbiota in the context of POF will enable a deeper understanding of the disease pathogenesis that involves modification of the vaginal microbiota. Conclusions: The present study identified the microbiota associated with POF. Further investigations on the differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involves modification of the vaginal microbiota.

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Section: Discussionmentioning

confidence: 99%

Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

BMC Microbiol

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“…They also found extensive differences in lipid composition between the EVs of the asthma group and controls. Lipids such as ceramides, ceramide‐phosphates, phosphatidylglycerol and sphingomyelins, which are mediators of inflammation in asthma, were found to be increased in EVs derived from asthma patients …”

Section: Resultsmentioning

confidence: 99%

“…. A study by Hough et al74 compared the lipid composition and presence of specific lipid mediators in airway EVs purified from the BALF of healthy controls and asthmatic subjects. The results indicated elevated concentrations of EVs in the BALF of asthmatic subjects and that the increase correlated with blood eosinophilia.…”

mentioning

confidence: 99%

“…They also found extensive differences in lipid composition between the EVs of the asthma group and controls. Lipids such as ceramides, ceramide-phosphates, phosphatidylglycerol and sphingomyelins, which are mediators of inflammation in asthma, were found to be increased in EVs derived from asthma patients 74. A study by Rollet-Cohen et al75 used proteomics to compare respiratory exosomal contents isolated from BALF of patients suffering from cystic fibrosis (CF), primary ciliary dyskinesia (PCD) and asthma.…”

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confidence: 99%

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Extracellular vesicles and asthma: A review of the literature

Sangaphunchai

Todd

Fairclough

2020

Clin Experimental Allergy

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Asthma is a chronic, recurrent and incurable allergy‐related respiratory disease characterized by inflammation, bronchial hyperresponsiveness and narrowing of the airways. Extracellular vesicles (EVs) are a universal feature of cellular function and can be detected in different bodily fluids. Recent evidence has shown the possibility of using EVs in understanding the pathogenesis of asthma, including their potential as diagnostic and therapeutic tools. Studies have reported that EVs released from key cells involved in asthma can induce priming and activation of other asthma‐associated cells. A literature review was conducted on all current research regarding the role and function of EVs in the pathogenesis of asthma via the PRISMA statement method. An electronic search was performed using EMBASE and PubMed through to November 2018. The EMBASE search returned 76 papers, while the PubMed search returned 211 papers. Following duplicate removal, titles and abstracts were screened for eligibility with a total of 34 studies included in the final qualitative analysis. The review found evidence of association between the presence of EVs and physiological changes characteristic of asthma, suggesting that EVs are involved in the pathogenesis, with the weight of evidence presently favouring deleterious effects of EVs in asthma. Numerous studies highlighted differences in exosomal contents between EVs of healthy and asthmatic individuals, which could be employed as potential diagnostic markers. In some circumstances, EVs were also found to be suppressive to disease, but more often promote inflammation and airway remodelling. In conclusion, EVs hold immense potential in understanding the pathophysiology of asthma, and as diagnostic and therapeutic markers. While more research is needed for definitive conclusions and their application in medical practice, the literature presented in this review should encourage further research and discovery within the field of EVs and asthma.

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“…For instance, EVs from non-tumorigenic cells are enriched in prenol lipids, glycerolipids, and fatty acids, while EVs from tumorigenic cells have higher levels of sphingolipids, glycerophospholipids, and sterol lipids [ 76 ]. Similarly, EVs from the bronchoalveolar lavage fluid of asthmatic patients are enriched in ceramide-phosphates, ceramides, and phosphatidylglycerol in comparison to healthy controls [ 77 ]. Even different subtypes of EVs can have distinctive lipidomic profiles.…”

Section: Biochemical Properties Of Evs and Their Intrinsic Advantamentioning

confidence: 99%

Extracellular Vesicles as an Efficient and Versatile System for Drug Delivery

Dang

Kavishka

Zhang

et al. 2020

Cells

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Despite the recent advances in drug development, the majority of novel therapeutics have not been successfully translated into clinical applications. One of the major factors hindering their clinical translation is the lack of a safe, non-immunogenic delivery system with high target specificity upon systemic administration. In this respect, extracellular vesicles (EVs), as natural carriers of bioactive cargo, have emerged as a promising solution and can be further modified to improve their therapeutic efficacy. In this review, we provide an overview of the biogenesis pathways, biochemical features, and isolation methods of EVs with an emphasis on their many intrinsic properties that make them desirable as drug carriers. We then describe in detail the current advances in EV therapeutics, focusing on how EVs can be engineered to achieve improved target specificity, better circulation kinetics, and efficient encapsulation of therapeutic payloads. We also identify the challenges and obstacles ahead for clinical translation and provide an outlook on the future perspective of EV-based therapeutics.

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Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics

Cited by 97 publications

References 112 publications

Changes in the vaginal microbiota associated with primary ovarian failure

Changes in the vaginal microbiota associated with primary ovarian failure

Extracellular vesicles and asthma: A review of the literature

Extracellular Vesicles as an Efficient and Versatile System for Drug Delivery

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