Unique Homologous siRNA Blocks Hypoxia-Induced VEGF Upregulation in Human Corneal Cells and Inhibits and Regresses Murine Corneal Neovascularization

Singh, Nirbhai; Higgins, Eric; Amin, Shivan; Jani, Pooja D.; Richter, Elizabeth R.; Patel, Ankit R.; Kaur, Rajwinder; Wang, Jin; Ambati, Jayakrishna; Zheng, Dezhi; Ambati, Balamurali K.

doi:10.1097/ico.0b013e31802b4201

Cited by 104 publications

(52 citation statements)

References 25 publications

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“…Each cohort consisted of 8-10 mice. Animals healing and reduce scarring (31). A recent study investigating the effects of a mixed cell population consisting were culled 4 weeks after commencing the experiment and blood and liver tissue collected.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl₄-Treated Mice

et al. 2010

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Chronic liver injury and inflammation lead to hepatic fibrosis, cirrhosis, and liver failure. Embryonic and mesenchymal stem cells have been shown to reduce experimental liver fibrosis but have potential limitations, including the formation of dysplastic precursors, tumors, and profibrogenic cells. Other stem-like cells may reduce hepatic inflammation and fibrosis without tumor and profibrogenic cell formation. To test this hypothesis we transplanted human amnion epithelial cells (hAEC), isolated from term delivered placenta, into immunocompetent C57/BL6 mice at week 2 of a 4-week regimen of carbon tetrachloride (CCl4) exposure to induce liver fibrosis. Two weeks following hAEC infusion, intact cells expressing the human-specific markers inner mitochondrial membrane protein and human leukocyte antigen-G were found in mouse liver without evidence of host rejection of the transplanted cells. Human albumin, known to be produced by hAEC, was detected in sera of hAEC-treated mice. Human DNA was detected in mouse liver and also spleen, lungs, and heart of some animals. Following hAEC transplantation, CCl4-treated animals showed decreased serum ALT levels and reduced hepatocyte apoptosis, compared to controls. hAEC-treated mouse liver had lower TNF-α and IL-6 protein levels and higher IL-10 compared to animals given CCl4 alone. Compared to CCl4 controls, hAEC-treated mice showed fewer activated collagen-producing hepatic stellate cells and less fibrosis area and collagen content. Reduced hepatic TGF-β levels in conjunction with a twofold increase in the active form of the collagen-degrading enzyme matrix metalloproteinase-2 in hAEC-treated mice compared to CCl4 controls may account for the reduction in fibrosis. hAEC transplantation into immunocompetent mice leads to cell engraftment, reduced hepatocyte apoptosis, and decreased hepatic inflammation and fibrosis.

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Section: Introductionmentioning

confidence: 99%

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl₄-Treated Mice

et al. 2010

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“…The utility of this new biomaterial may be because the HAM promotes epithelialization and neovascularization and possesses immune privilege (Sippel et al 2001). It has been previously documented that HAMs may accelerate epithelialization of gingival wounds and ocular chemical and thermal injures when reconstructing damaged organs and corneal tissue (Rinastiti et al 2006;Madhira et al 2008;Sangwan et al 2007;Tejwani et al 2007). Also, Santos et al (2005) have demonstrated the suitability of HAMs for treating limbal stem cell deficiency (LSCD).…”

Section: Discussionmentioning

confidence: 96%

Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair

et al. 2010

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The human amniotic membrane (HAM) is an abundant and readily obtained tissue that may be an important source of scaffold for transplanted chondrocytes in cartilage regeneration in vivo. To evaluate the potential use of cryopreserved HAMs as a support system for human chondrocytes in human articular cartilage repair. Chondrocytes were isolated from human articular cartilage, cultured and grown on the chorionic basement membrane side of HAMs. HAMs with chondrocytes were then used in 44 in vitro human osteoarthritis cartilage repair trials. Repair was evaluated at 4, 8 and 16 weeks by histological analysis. Chondrocytes cultured on the HAM revealed that cells grew on the chorionic basement membrane layer, but not on the epithelial side. Chondrocytes grown on the chorionic side of the HAM express type II collagen but not type I, indicating that after being in culture for 3-4 weeks they had not de-differentiated into fibroblasts. In vitro repair experiments showed formation on OA cartilage of new tissue expressing type II collagen. Integration of the new tissue with OA cartilage was excellent. The results indicate that cryopreserved HAMs can be used to support chondrocyte proliferation for transplantation therapy to repair OA cartilage.

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“…The largest size possible, allowing closure of eyelids, is preferable in order to prevent adhesions and shortening of the fornices without touching the cornea (4) . Several studies have reported successful treatment of ocular burns using AMT with or without limbal cell transplantation (13)(14)(15)(16)(17)(18) . AMT alone was reported to be efficient in terms of restoring ocular surfaces in cases of mild to moderate burns (14,16) .…”

Section: Discussionmentioning

confidence: 99%

Molten metal-related ocular thermal burn: report on two cases

Arıcı¹,

İskeleli²,

Atalay³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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We report two cases of severe thermal burns on the ocular surface and its adnexal appendages that developed secondary to exposure to molten heavy metal with a melting temperature of near-thousand degree Celsius. Despite aggressive intervention and strict monitoring, the profound inflammation caused significant damage to the ocular surface, ending up in an intractable infection with an unfavorable outcome. The heat of the molten metal at impact, the heat-retaining capacity of the heavy metal, the total area of the ocular surface exposed to the molten metal, and the duration of exposure determined the severity of the injury. The unfavorable outcome, despite an intensive treatment, in terms of visual acuity and cosmetic appearance, should be explicitly explained to the patient, and a psychiatrist consultation should be considered if necessary.

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Unique Homologous siRNA Blocks Hypoxia-Induced VEGF Upregulation in Human Corneal Cells and Inhibits and Regresses Murine Corneal Neovascularization

Cited by 104 publications

References 25 publications

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl₄-Treated Mice

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl₄-Treated Mice

Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair

Molten metal-related ocular thermal burn: report on two cases

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Unique Homologous siRNA Blocks Hypoxia-Induced VEGF Upregulation in Human Corneal Cells and Inhibits and Regresses Murine Corneal Neovascularization

Cited by 104 publications

References 25 publications

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl4-Treated Mice

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl4-Treated Mice

Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair

Molten metal-related ocular thermal burn: report on two cases

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Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl₄-Treated Mice

Transplantation of Human Amnion Epithelial Cells Reduces Hepatic Fibrosis in Immunocompetent CCl₄-Treated Mice