Unique Aspects of Plant Nonsense-Mediated mRNA Decay

Shaul, Orit

doi:10.1016/j.tplants.2015.08.011

Cited by 88 publications

(73 citation statements)

References 106 publications

(268 reference statements)

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“…1). Several mRNA qualities that compromise the integrity of translation termination trigger NMD, including a PTC marked by the presence of an exon junction complex 39 of a stop codon (Reddy et al, 2013;Shaul, 2015). This is often a hallmark of incomplete or alternative splicing.…”

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confidence: 99%

“…The early steps of PTC recognition and NMD complex formation in eukaryotes involve UP FRAMESHIFT1 (UPF1), UPF2, UPF3, and SUPPRESSOR WITH MOR-PHOLOGICAL EFFECT ON GENITALIAs (SMGs; for review, see Shaul, 2015;Dai et al, 2016). The late steps of NMD involve translational repression and the degradation of target mRNAs.…”

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confidence: 99%

“…The late steps of NMD involve translational repression and the degradation of target mRNAs. In mammals, the degradation of NMD targets is initiated by SMG6-mediated endonucleolytic cleavage followed by exonucleolytic degradation of the 39 and 59 cleavage products by the 59-39 exoribonuclease EXORIBONUCLEASE1 (XRN1) and the exosome (see below), respectively (Shaul, 2015). In Arabidopsis (Arabidopsis thaliana), an ortholog of SMG6 has not been identified, but degradation of NMD substrates occurs in PBs and requires UPF1 and SMG7.…”

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confidence: 99%

“…It also tempers levels of defense-related transcripts under normal growth conditions (Jeong et al, 2011;Gloggnitzer et al, 2014;Shaul, 2015), contributes to the repression of EIN3-BINDING F BOX PROTEIN2 mRNA translation in response to ethylene (Merchante et al, 2015), and promotes the acclimation response to salt stress in Arabidopsis (Vexler et al, 2016). A general dampening of NMD by stresses could be responsible for the increase in NMDsensitive splicing variants in response to high salt (Drechsel et al, 2013) and prematurely polyadenylated transcripts under hypoxia (de Lorenzo et al, 2017).…”

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confidence: 99%

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Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

2017

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Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

2017

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“…In plants, in addition to the UPF1-3 proteins, orthologs of SMG7, SMG1, and the four core EJC components have been experimentally identified (Lloyd and Davies, 2013;Mérai et al, 2013;Nyikó et al, 2013;Shaul, 2015). Some of the regulatory steps of NMD have also been identified.…”

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confidence: 99%

The RNA Polymerase II C-Terminal Domain Phosphatase-Like Protein FIERY2/CPL1 Interacts with eIF4AIII and Is Essential for Nonsense-Mediated mRNA Decay in Arabidopsis

et al. 2016

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Nonsense-mediated decay (NMD) is a posttranscriptional surveillance mechanism in eukaryotes that recognizes and degrades transcripts with premature translation-termination codons. The RNA polymerase II C-terminal domain phosphatase-like protein FIERY2 (FRY2; also known as C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 [CPL1]) plays multiple roles in RNA processing in Arabidopsis thaliana. Here, we found that FRY2/CPL1 interacts with two NMD factors, eIF4AIII and UPF3, and is involved in the dephosphorylation of eIF4AIII. This dephosphorylation retains eIF4AIII in the nucleus and limits its accumulation in the cytoplasm. By analyzing RNA-seq data combined with quantitative RT-PCR validation, we found that a subset of alternatively spliced transcripts and 59-extended mRNAs with NMD-eliciting features accumulated in the fry2-1 mutant, cycloheximidetreated wild type, and upf3 mutant plants, indicating that FRY2 is essential for the degradation of these NMD transcripts.

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A Negative Feedback Loop Compromises NMD‐Mediated Virus Restriction by the Autophagy Pathway in Plants

Chen,

Jia,

et al. 2024

Advanced Science

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Nonsense‐mediated decay (NMD) and autophagy play pivotal roles in restricting virus infection in plants. However, the interconnection between these two pathways in viral infections has not been explored. Here, it is shown that overexpression of NbSMG7 and NbUPF3 attenuates cucumber green mottle mosaic virus (CGMMV) infection by recognizing the viral internal termination codon and vice versa. NbSMG7 is subjected to autophagic degradation, which is executed by its interaction with one of the autophagy‐related proteins, NbATG8i. Mutation of the ATG8 interacting motif (AIM) in NbSMG7 (SMG7mAIM1) abolishes the interaction and comprises its autophagic degradation. Silencing of NbSMG7 and NbATG8i, or NbUPF3 and NbATG8i, compared to silencing each gene individually, leads to more virus accumulations, but overexpression of NbSMG7 and NbATG8i fails to achieve more potent virus inhibition. When CGMMV is co‐inoculated with NbSMG7mAIM1 or with NbUPF3, compared to co‐inoculating with NbSMG7 in NbATG8i transgene plants, the inoculated plants exhibit milder viral phenotypes. These findings reveal that NMD‐mediated virus inhibition is impaired by the autophagic degradation of SMG7 in a negative feedback loop, and a novel regulatory interplay between NMD and autophagy is uncovered, providing insights that are valuable in optimizing strategies to harness NMD and autophagy for combating viral infections.

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Unique Aspects of Plant Nonsense-Mediated mRNA Decay

Cited by 88 publications

References 106 publications

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

The RNA Polymerase II C-Terminal Domain Phosphatase-Like Protein FIERY2/CPL1 Interacts with eIF4AIII and Is Essential for Nonsense-Mediated mRNA Decay in Arabidopsis

A Negative Feedback Loop Compromises NMD‐Mediated Virus Restriction by the Autophagy Pathway in Plants

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