Unique antioxidant effects of herbal leaf tea and stem tea fromMoringa oleiferaL. especially on superoxide anion radical generation systems

Abstract

This study aimed to investigate the unique antioxidative effects of Japanese moringa products, herbal leaf tea and stem tea, using established free radical assays, focusing on superoxide anion (O) radical generation systems. Hot-water extracts from moringa teas resulted in different but lower scavenging activities than Trolox in four synthetic free radical models. Interestingly, these extracts further showed higher O radical scavenging effects than Trolox in the phenazine methosulfate-NADH-nitroblue tetrazolium and xanthine oxidase assay systems. Incubating human neutrophils in the presence of these tea extracts rather than Trolox effectively suppressed cellular O radical generation. Among the eight known phenolic constituents of moringa leaves, caffeic acid and chlorogenic acid may be responsible for the Ospecific radical scavenging capacity stronger than that of Trolox. These results suggest that moringa herbal teas are a good source of natural antioxidants for preventing O radical-mediated disorders. Abbreviations: O: superoxide anion; ROS: reactive oxygen species; HO: hydrogen peroxide; XOD: xanthine oxidase; DPPH: 1,1-diphenyl-2-picrylhydrazyl; ABTS: 2,2'-azinobis(2-ethylbenzothiazoline-6-sulfonic acid) cation; CPZ: chlorpromazine cation; PMS: phenazine methosulfate; NBT: nitroblue tetrazolium; PMA: phorbol 12-myristate 13-acetate.

Optimization of Hot-water Extraction of Dried Yacon Herbal Tea Leaves: Enhanced Antioxidant Activities and Total Phenolic Content by Response Surface Methodology

“…In our recent studies, commercial yacon leaf tea, and moringa leaf and stem herbal teas exhibited stronger O 2 _ radical scavenging capacity than trolox (Sugahara et al, 2015;. Several representative polyphenols can demonstrate SOD-like O 2 _ radical scavenging capacity, e.g., phenolic acids including caffeic, chlorogenic and gallic acids, and flavonoids such as epicatechin, kaempferol, myricetin, quercetin and rutin (Sugahara et al, 2018). Ferric reducing capability is a target in determining the antioxidant activity of test samples as well as biological fluids such as plasma (Benzie and Strain, 1996;1999).…”

Anti-Inflammatory and Hypouricemic Effect of Bioactive Compounds: Molecular Evidence and Potential Application in the Management of Gout

“…The decrease of uric acid levels occurs mainly through the inhibition of xanthine oxidase (XOD) and adenosine deaminase (ADA), enzymes that regulate the metabolic pathway leading to uric acid synthesis ( Figure 2 ). In particular, the ability to inhibit XOD has been investigated for a large number of molecules including caffeic acid [ 12 ], apigenin, quercetin, luteolin [ 13 , 14 ], kaempferol [ 15 ], rutin [ 16 ], chlorogenic acid [ 17 ], sinapic acid [ 18 ], phloroglucinol derivatives [ 19 ], and polyphenol-rich plant extracts [ 20 , 21 ]. Among the latter, extracts from tea leaves, Tartary buckwheat, Chrysanthemum morifolium, Smilax china L., Flourensia fiebrigii, and Keladi Candik, that contain a mix of many different bioactive compounds, have shown a high XOD inhibitory activity [ 22 , 23 , 24 , 25 , 26 ].…”

A Review of the Health Protective Effects of Phenolic Acids against a Range of Severe Pathologic Conditions (Including Coronavirus-Based Infections)

“…Caffeic acid (CA) is found at high levels in various herbs worldwide, including the South American herb yerba mate (1.5 g/kg) [ 16 ], the Japanese herbal leaf tea, the tea stem from Moringa oleifera L. [ 17 ], and thyme (1.7 mg/kg) [ 18 ].…”