Unique animal friendly 3D culturing of human cancer and normal cells

Permlid, Atena Malakpour; Roci, Plaurent; Fredlund, Elina; Fält, Felicia; Önell, Emil; Johansson, Fredrik; Oredsson, Stina

doi:10.1016/j.tiv.2019.04.022

Cited by 17 publications

(26 citation statements)

References 34 publications

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“…The cells were successfully grown over a 14-day span and the results indicated that the hybrid PLLCL/Col scaffold promotes cellular adhesion and proliferation even during long-term studies much more effectively than standard 2D cell culture models (Türker et al, 2019). Permlid et al (2019) demonstrated a novel animal-friendly 3D electrospun polycaprolaceton (PCL) synthetic scaffold that effectively mimicked the collagen network of tissue. Human breast cancer cell lines JIMT-1 and MCF-7, the normal-like breast epithelial MCF-10A cell line, and mouse L929 fibroblasts were seeded in the 3D PCL scaffold and incubated for 7 days (Permlid et al, 2019).…”

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

“…Permlid et al (2019) demonstrated a novel animal-friendly 3D electrospun polycaprolaceton (PCL) synthetic scaffold that effectively mimicked the collagen network of tissue. Human breast cancer cell lines JIMT-1 and MCF-7, the normal-like breast epithelial MCF-10A cell line, and mouse L929 fibroblasts were seeded in the 3D PCL scaffold and incubated for 7 days (Permlid et al, 2019). After 7 days, the 3D cultures were analyzed using scanning electron microscopy, confocal laser scanning microscopy, and cryosectioning (Permlid et al, 2019).…”

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

“…Human breast cancer cell lines JIMT-1 and MCF-7, the normal-like breast epithelial MCF-10A cell line, and mouse L929 fibroblasts were seeded in the 3D PCL scaffold and incubated for 7 days (Permlid et al, 2019). After 7 days, the 3D cultures were analyzed using scanning electron microscopy, confocal laser scanning microscopy, and cryosectioning (Permlid et al, 2019). The results showed that both the malignant as well as normal cell lines flourished in the 3D PCL scaffold indicating the potential to create a tumor ex vivo platform to screen therapeutic compounds (Permlid et al, 2019).…”

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

“…After 7 days, the 3D cultures were analyzed using scanning electron microscopy, confocal laser scanning microscopy, and cryosectioning (Permlid et al, 2019). The results showed that both the malignant as well as normal cell lines flourished in the 3D PCL scaffold indicating the potential to create a tumor ex vivo platform to screen therapeutic compounds (Permlid et al, 2019). Sankar et al (2019) used ES in conjunction with photolithography to fabricate both nano-and micro-patterned PLGA/Collagen/nHAp fiber mats.…”

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

See 3 more Smart Citations

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Jensen

Teng

2020

Front. Mol. Biosci.

1,029

802

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Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

Section: Dp Scaffolds For 3d Cell Culture Via Electrospinningmentioning

confidence: 99%

See 2 more Smart Citations

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Jensen

Teng

2020

Front. Mol. Biosci.

1,029

802

View full text Add to dashboard Cite

“…Among the fabrication technologies applied to process PCL into 3D scaffolds, electrospinning is a cost-effective and simple process that allows the formation of polymer fibers with sub-micron diameter, mimicking the native extracellular matrix (ECM) microstructures, especially the high-ordered collagen microfibrils [ 11 ]. Thus, thanks to their porosity, electrospun fiber meshes provide a natural 3D tissue-like structure enabling oxygen and nutrient gradients [ 11 , 12 ]. Various electrospun PCL fibrous scaffolds have been already evaluated to obtain 3D in vitro breast cancer models useful for drug screening and reducing the number of animals in cancer research [ 3 , 7 , 11 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Breast Cancer Cell Cultures on Electrospun Poly(ε-Caprolactone) as a Potential Tool for Preclinical Studies on Anticancer Treatments

et al. 2020

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During anticancer drug development, most compounds selected by in vitro screening are ineffective in in vivo studies and clinical trials due to the unreliability of two-dimensional (2D) in vitro cultures that are unable to mimic the cancer microenvironment. Herein, HCC1954 cell cultures on electrospun polycaprolactone (PCL) were characterized by morphological analysis, cell viability assays, histochemical staining, immunofluorescence, and RT-PCR. Our data showed that electrospun PCL allows the in vitro formation of cultures characterized by mucopolysaccharide production and increased cancer stem cell population. Moreover, PCL-based cultures were less sensitive to doxorubicin and electroporation/bleomycin than those grown on polystyrene plates. Collectively, our data indicate that PCL-based cultures may be promising tools for preclinical studies.

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Recent advances in scaffold based electrospun for breast cancer research

Ebrahimbaygi,

Khazaei,

Valadbeigi

et al. 2024

Polymers for Advanced Techs

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One of the biggest challenges facing public health in the modern era is the management of cancer, a global health issue. Breast cancer (BC) is the most common malignancy among women worldwide. Among the most popular cancer treatment modalities are radiation, chemotherapy, and surgery. Chemotherapy, however, is regarded as the primary treatment option for cancer that has progressed to the final and metastatic stages. However, because of things like toxicity to healthy cells, poor drug absorption, trouble getting drugs to target tumor sites, and low therapeutic efficacy, traditional chemotherapy approaches are frequently insufficient. Nanotechnology offers the potential to overcome some of these limitations by creating new materials with unique properties through electrospinning. A straightforward and reasonably priced technique for creating biomaterials that can replicate the topography and structure of the cellular matrix is electrospinning. These materials have a large surface area, can be mechanically controlled, and have a level of fibers that can be adjusted from micrometers to nanometers. This review article emphasizes the potential of electrospun scaffolds for the treatment of BC while also offering a basic understanding of the procedure and nanostructured fibrous materials. This article reviews the most recent emerging electrospinning techniques in BC therapy. First, it briefly introduces the progress made in electrospinning in BC research over the past few years. Next, it investigates electrospinning by summarizing the techniques and materials used in the process. In addition, it demonstrates how crucial electrospinning three‐dimensional (3D) models are for the diagnosis and treatment of BC. This article also clarifies numerous electrospinning uses in several fields, such as cell culture, drug delivery, drug loading, and gene therapy. The final section discusses the advantages, limitations, and challenges electrospinning is willing to encounter in BC research.

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Unique animal friendly 3D culturing of human cancer and normal cells

Cited by 17 publications

References 34 publications

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Breast Cancer Cell Cultures on Electrospun Poly(ε-Caprolactone) as a Potential Tool for Preclinical Studies on Anticancer Treatments

Recent advances in scaffold based electrospun for breast cancer research

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