Unique Allergic Asthma Phenotypes in Offspring of House Dust Mite–exposed Mice

Lebold, Katie M.; Drake, Matthew G.; Pincus, Alexandra; Pierce, A.; Fryer, A.D.; Jacoby, David B.

doi:10.1165/rcmb.2021-0535oc

Cited by 9 publications

(7 citation statements)

References 41 publications

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“…Behavioural outcomes included locomotion [ 50 , 73 , 74 ], social activity [ 50 , 73 , 74 ], anxiety-like behaviours [ 50 , 73 , 74 ] and repetitive behaviours [ 73 , 74 ]. Nonlung organ outcomes include neuronal morphology [ 32 , 72 ] and adrenal medulla histology and protein expression [ 31 , 75 ]. Genetic and epigenetic outcomes included whole-genome DNA methylation changes [ 62 ], genotype profiling of splenic dendritic cells [ 62 ], gene expression in airways [ 76 ] and the microglial transcriptome [ 77 ].…”

Section: Resultsmentioning

confidence: 99%

“…Fewer studies (15%) sensitised and challenged mothers with HDM, which is the most prevalent allergen in human asthma [ 80 ]. Allergic asthma can be further divided into a mild–moderate phenotype driven by T-helper type 2 cells (Th2), or a moderate–severe phenotype driven by T-helper type 1 or 17 cells (Th1/Th17) [ 72 , 81 ]. Importantly, the specific cells involved in the allergic immune response can alter progeny outcomes.…”

Section: Discussionmentioning

confidence: 99%

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Preclinical models of maternal asthma and progeny outcomes: a scoping review

Robinson,

Gatford,

Bailey

et al. 2024

Eur Respir Rev

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There is an increased risk of adverse perinatal outcomes in the ∼17% of women with asthma during pregnancy. The mechanisms linking maternal asthma and adverse outcomes are largely unknown, but reflect joint effects of genetics and prenatal exposure to maternal asthma. Animal models are essential to understand the underlying mechanisms independent of genetics and comorbidities, and enable safe testing of interventions. This scoping review aimed to explore the methodology, phenotype, characteristics, outcomes and quality of published studies using preclinical maternal asthma models. MEDLINE (PubMed), Embase (Elsevier) and Web of Science were systematically searched using previously validated search strings for maternal asthma and for animal models. Two reviewers independently screened titles and abstracts, full texts, and then extracted and assessed the quality of each study using the Animal Research: Reporting ofIn VivoExperiments (ARRIVE) 2.0 guidelines. Out of 3618 studies identified, 39 were eligible for extraction. Most studies were in rodents (86%) and all were models of allergic asthma. Maternal and progeny outcomes included airway hyperresponsiveness, airway resistance, inflammation, lung immune cells, lung structure and serum immunoglobulins and cytokines. Experimental design (100%), procedural details (97%) and rationale (100%) were most often reported. Conversely, data exclusion (21%), blinding (18%) and adverse events (8%) were reported in a minority of studies. Species differences in physiology and timing of development, the use of allergens not relevant to humans and a lack of comparable outcome measures may impede clinical translation. Future studies exploring models of maternal asthma should adhere to the minimum core outcomes set presented in this review.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Preclinical models of maternal asthma and progeny outcomes: a scoping review

Robinson,

Gatford,

Bailey

et al. 2024

Eur Respir Rev

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“…19 Further evidence of immune programming of T cells comes from another murine model of maternal asthma where asthmatic offspring exhibited a moderate to severe T H 1/T H 17 inflammatory signature in response to allergen challenge, compared with the milder T H 2 signature of their mothers. 20 In sheep, near-term fetuses from asthmatic mothers had lower lung surfactant expression, lower type II alveolar epithelial cell density, and higher proportions of CD44 positive lymphocytes in the thymus, 21,22 which could lead to poorer neonatal lung function and greater inflammatory response, respectively. This evidence from preclinical models suggests that the in utero environment of an asthmatic mother alters lung and immune development of progeny.…”

Section: Introductionmentioning

confidence: 99%

“…In this model, reduced maternal ovalbumin exposure pre-mating or blocking interleukin 4 (IL-4) by anti-mouse IL-4 antibody prevented adverse respiratory outcomes for the offspring of asthmatic mothers 19 . Further evidence of immune programming of T cells comes from another murine model of maternal asthma where asthmatic offspring exhibited a moderate to severe T H 1/T H 17 inflammatory signature in response to allergen challenge, compared with the milder T H 2 signature of their mothers 20 . In sheep, near-term fetuses from asthmatic mothers had lower lung surfactant expression, lower type II alveolar epithelial cell density, and higher proportions of CD44 positive lymphocytes in the thymus, 21,22 which could lead to poorer neonatal lung function and greater inflammatory response, respectively.…”

Section: Introductionmentioning

confidence: 99%

Preclinical models of maternal asthma and progeny outcomes: a scoping review protocol

Robinson

Gatford

Clifton

et al. 2023

JBI Evidence Synthesis

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Objective: This scoping review will describe the methodology, phenotype, and characteristics of maternal asthma models used in preclinical studies and the outcomes that have been measured in the mother and progeny. The review This will identify gaps in knowledge of maternal and progeny outcomes following maternal asthma in pregnancy. Introduction: Maternal asthma affects up to 17% of pregnancies worldwide and is associated with adverse perinatal outcomes in mothers and babies, including pre-eclampsia, gestational diabetes, cesarean section, preterm birth, small for gestational age, nursery admission, and neonatal death. While the associations are well established, the mechanisms linking maternal asthma and adverse perinatal outcomes are largely unknown due to the difficulties of human mechanistic studies. The appropriate selection of animal models is vital to understanding the mechanisms underlying associations between human maternal asthma and adverse perinatal outcomes. Inclusion criteria: This review will include primary studies published in English where outcomes have been studied in vivo in non-human mammalian species. Methods: This review will follow the JBI methodology for scoping reviews. We will search MEDLINE (PubMed), Embase, and Web of Science to identify papers published before the end of 2022. Initial keywords will include pregnancy, gestation, asthma, and wheeze, as well as validated search strings to identify papers that describe animal models. Extracted data will include information on methods used to induce maternal asthma; asthmatic phenotypes and characteristics; and maternal, pregnancy, placental, and progeny outcomes. The characteristics of each study will be presented in summary tables and a core outcome list to assist researchers in developing, reporting, and comparing future animal studies of maternal asthma. Review registration: Open Science Framework osf.io/trwk5

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“…In this issue of the Journal , Lebold and colleagues (pp. 89–98 ) report on their investigations of allergic airway inflammation and airway hyperresponsiveness (AHR) in mouse pups born from allergen-challenged dams ( 7 ). In the model, female mice were chronically challenged with PBS or a common household allergen, house dust mite (HDM), before and during pregnancy.…”

mentioning

confidence: 99%