Uniparental Mitochondrial Transmission in Sexual Crosses in Cryptococcus neoformans

Xu, Jianping; Ali, Rabia Y.; Gregory, Devon A.; Amick, Denise; Lambert, Sarah E.; Yoell, Heather J.; Vilgalys, Rytas; Mitchell, Thomas G.

doi:10.1007/s002849910053

Cited by 65 publications

(75 citation statements)

References 18 publications

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“…The second is that these introns appeared in these locations after separation of these species. Analysis of the mt large rRNA gene and NADH dehydrogenase subunit 2 of C. neoformans revealed that serotype AD strains had either the serotype A or serotype D mtDNA genotype (29). In the present study, we obtained almost similar results for the distribution of cyt b gene types in serotype AD strains.…”

Section: Discussionsupporting

confidence: 80%

“…Restriction fragment length polymorphism (RFLP) analysis of mtDNA has been shown to be useful for estimating the relations between fungi (8, 10, 24). Xu et al (29) showed that the origin of mitochondria in C. neoformans is uniparental. RFLP analysis of the mt large rRNA gene and NADH dehydrogenase subunit 2 gene allowed efficient screening of the mtDNA of C. neoformans (28).…”

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confidence: 99%

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Molecular Analysis of Cryptococcus neoformans Mitochondrial Cytochrome b Gene Sequences

et al. 2003

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Section: Discussionsupporting

confidence: 80%

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confidence: 99%

Molecular Analysis of Cryptococcus neoformans Mitochondrial Cytochrome b Gene Sequences

et al. 2003

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“…Unilateral production of conjugation tubes by MATa, unidirectional transfer of nuclei from MATa to MATa (McClelland et al, 2004), uniparental (MATa specific) inheritance of mitochondria (Xu et al, 2000), presence of mating type-specific pheromone response MAP kinase cascade genes, and the phenotypic differences revealed between tup1 strains of the two mating types point to the dioecism in C. neoformans. Dioecism by definition denotes the separation of sexes: presence of male and female sex organs in two different individuals and sexual reproduction requiring nuclear transfer from male to female (Alexopoulos et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Dikaryotic hyphae are subsequently produced normally by the MATa cells and meiotic products (basidiospores) are borne on the resulting basidia (KwonChung, 1980;McClelland et al ., 2004) which mimics the situation in dioecious plants and animals. In support of this concept, mitochondrial inheritance in C. neoformans has been shown to be uniparental: meiotic products carry only MATa type mitochondria (Xu et al ., 2000).…”

Section: Introductionmentioning

confidence: 96%

TUP1 disruption reveals biological differences between MATa and MATα strains of Cryptococcus neoformans

Lee

Chang

Kwon‐Chung

2005

Molecular Microbiology

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SummaryCryptococcus neoformans exists in two mating types MATa and MAT a a a a . Although the morphology, growth characteristics and genetic segregation patterns among MATa and MAT a a a a strains are indistinguishable in the laboratory, the predominance of MAT a a a a strains in nature suggests that MAT a a a a strains are better suited for survival in nature. We disrupted the TUP1 gene, a global repressor, to find the possible biological differences in congenic MAT a a a a and MATa cells of C. neoformans. Disruption of TUP1 affected neither the yeast nor the hyphal cell morphology but resulted in a similar reduction of mating frequencies in both MAT a a a a and MATa cells. Disruption of TUP1 , however, functionally manifested itself in several mating type-dependent phenotypes: (i) MAT a a a a cells became more sensitive to 0.8 M KCl while MATa cells showed no change in sensitivity, (ii) a temperature-dependent growth reduction was exhibited at both 30 ∞ ∞ ∞ ∞ C and 25 ∞ ∞ ∞ ∞ C in MATa but a similar growth reduction was not observed in MAT a a a a cells until the temperature was lowered to 25 ∞ ∞ ∞ ∞ C and (iii) the transcriptional level of genes in several different biological pathways was markedly altered in a mating type-dependent manner. This work is the first case in which non-mating-related biological differences are observed between two congenic mating partners in yeast.

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“…First, strains of serotype A isolated from either nature or patients can mate with serotype D strains (e.g. Xu et al, 2000a). Second, clinical isolates of serotype AD typically exhibit heterozygosity at several enzymic loci, sharing alleles from both serotypes A and D strains (Brandt et al, 1993(Brandt et al, , 1995.…”

Section: Introductionmentioning

confidence: 99%

Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD

Luo

Vilgalys

et al. 2002

Microbiology

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Cryptococcus neoformans is a major pathogen of humans throughout the world. Using commercial mAbs to capsular epitopes, strains of C. neoformans manifest five distinct serotypes -A, B, C, D and AD. Previous studies demonstrated significant divergence among serotypes A, B, C and D, which are thought to be haploid. In this study the origins and evolution of strains of serotype AD were investigated. A portion (537 bp) of the laccase gene was cloned and sequenced from 14 strains of serotype AD. Each strain contained two different alleles and sequences for both alleles were obtained. These sequences were compared to those from serotypes A, B, C and D. This analysis indicated that each of the 14 serotype AD strains contained two phylogenetically distinct haplotypes : one haplotype was highly similar to the serotype A group and the other to the serotype D group. To explain the origins of these serotype AD strains, genealogical analysis is consistent with at least three recent and independent hybridization events. The results demonstrate that the evolution of C. neoformans is continuing and dynamic.

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Uniparental Mitochondrial Transmission in Sexual Crosses in Cryptococcus neoformans

Cited by 65 publications

References 18 publications

Molecular Analysis of Cryptococcus neoformans Mitochondrial Cytochrome b Gene Sequences

Molecular Analysis of Cryptococcus neoformans Mitochondrial Cytochrome b Gene Sequences

TUP1 disruption reveals biological differences between MATa and MATα strains of Cryptococcus neoformans

Multiple origins of hybrid strains of Cryptococcus neoformans with serotype AD

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