Uniparental, fusant, and chimeric plants regenerated from protoplasts after streak plating in agarose gels

Binding, Horst; B�nning, D; G�rschen, E.; J�rgensen, J.; Kollmann, R.; Krumbiegel-Schroeren, G.; Ling, H. O.; Monzer, J.; Mordhorst, G.; Rudnick, Joachim; Sauer, A.; Witt, D.; Zuba, M.

doi:10.1007/bf00040074

Cited by 9 publications

(2 citation statements)

References 3 publications

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“…Alternatively, the protoplasts were embedded in medium 8pmC.1 or 8pmC.2 mixed with 3% LM agarose (50°C) (Biozym Scientific, Hessisch Oldendorf, Germany) in a 2:1 ratio. This mixture was pipetted in lenses in 5.5-cm-diameter petri dishes (Binding et al 1988) and, after a 1-h solidification, these cultures were flooded with 3.5 ml culture medium 8pmC.1 or 8pmC.2. All protoplast cultures were incubated at 24 ± 1°C in the dark.…”

Section: Protoplast Isolation and Culturementioning

confidence: 99%

Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis

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Bartsch

et al. 2010

Plant Cell Tiss Organ Cult

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Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 9 10 5 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.

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Section: Protoplast Isolation and Culturementioning

confidence: 99%

Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis

Prange

Serek

Bartsch

et al. 2010

Plant Cell Tiss Organ Cult

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show abstract

“…St C--->S--->P Binding et al (1988) phyteuma ? Vardi et al (1975) Microcitrus australis x C sS C--->E--->P Vardi et al (1986) Gosch et al (1975) Browallia viscosa Schieder (1975Schieder ( ,1977 metel Schieder (1977) Hyoscyamus muticus et al (1979) Lycopersicon chi/ense (1983), L SoS C--->S--->P Haberlach et al (1985), Binding et al ( ,1981 isolation, the method of protoplast culture, the culture medium, and the physical environment.…”

Section: Protoplast Regenerationmentioning

confidence: 99%