Unintended Exposure to Shrimp Allergen: Studies of Cooking Oil used to Deep Fry Breaded Shrimp

Lehrer, Samuel B.; Oberhoff, S.W.; Klemawesch, P.J.; Jenson, L.; Rice, Timothy; Wünschmann, Sabina

doi:10.1016/j.jaci.2009.12.883

Cited by 2 publications

(1 citation statement)

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“…Allergenic foods are often present in trace amounts resulting from cross‐contamination which occurs, for example, in food processing plants through the use of shared equipment. In the case of seafood such as fish, cross‐contamination through the use of shared frying oil appears to have caused allergic reactions in consumers . Protection of public health therefore requires highly sensitive and specific methods for accurate detection of seafood allergens in food products, including oils and foods subjected to high‐temperature frying.…”

Section: Introductionmentioning

confidence: 99%

A method to detect allergenic fish, specifically cod and pollock, using quantitative real‐time PCR and COI DNA barcoding sequences

Eischeid

2018

J Sci Food Agric

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BACKGROUND: Fish are one of eight major allergens defined in the US Food Allergen Labeling and Consumer Protection Act, and cod and pollock are two of the major fish allergens. This paper describes development and validation of a method to detect cod and pollock in complex food matrices using real-time polymerase chain reaction (PCR). Mitochondrial cytochrome oxidase I (COI) sequences obtained through DNA barcoding were used to design a single set of primers and probe which detected three species in the genus Gadus: Atlantic cod, Pacific cod, and walleye pollock. RESULTS: Cod spiked into three different food matrices (cooking oil, clam chowder, and hushpuppy mix) yielded high linearity, dynamic range spanning six orders of magnitude, and lower limits of detection at 1-10 ppm (ppm; mg kg −1 ). Frying had an adverse effect on the lower limit of detection, but not on linearity.CONCLUSIONS: This work shows that COI DNA barcoding sequences can be used to effectively design real-time PCR assays for detection of food allergens in complex matrices. While full-length DNA barcodes distinguish individual species, the PCR assay designed here detected three different species. This is likely because real-time PCR assays are tolerant to basepair mismatches and do not utilize the full length of the DNA barcode. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.

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Section: Introductionmentioning

confidence: 99%