Unintended effects of genetic modifications and methods of their analysis in plants

Сорочинський, Б. В.; Burlaka, O. M.; Naumenko, V. D.; Секан, А. С.

doi:10.3103/s0095452711050124

Cited by 9 publications

(5 citation statements)

References 64 publications

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“…What's more, cross‐contamination is inevitable unless specific precautions are taken. Similar results were presented previously by Stram and others (2000), and they showed that GMO can be traced in turkey cutlet even though GMO products were not deliberately used in manufacturing. Therefore, the possibility of soy‐contaminated grain used in food producing process could not be easily ruled out.…”

Section: Resultssupporting

confidence: 89%

The Combination of Quantitative PCR and Western Blot Detecting CP4‐EPSPS Component in Roundup Ready Soy Plant Tissues and Commercial Soy‐Related Foodstuffs

Xiao

Zhou

et al. 2012

Journal of Food Science

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The combination of quantitative PCR (qPCR) and western blot was used to detect cp4-epsps gene and its protein product in different Roundup Ready soy (event 40-3-2) plant tissues and commercial soy-containing foodstuffs. The foods included those of plant origin produced by different processing procedures and also some products containing a combination of both meat and plant protein concentrates. This study indicated that the combination of DNA- and protein-based methods would supplement each other for genetically modified detection from nucleic acid and protein levels. Accordingly, qPCR and western blot could be used in CP4-EPSPS detection in a wide variety of soy-related foodstuffs.

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Section: Resultssupporting

confidence: 89%

The Combination of Quantitative PCR and Western Blot Detecting CP4‐EPSPS Component in Roundup Ready Soy Plant Tissues and Commercial Soy‐Related Foodstuffs

Xiao

Zhou

et al. 2012

Journal of Food Science

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“…Field studies are crucial to quantify phenotypic differences resulting from traditional plant breeding efforts. This is also true for genetically modified plants where there are many sources of genetic variation that may result in phenotypic differences between events, such as unstable gene expression related to placement and number of insertions in the genome, genetic rearrangement near the region where the transgene was inserted, silencing of the transgene by the host, and other unintended effects (Haslberger, 2003; Sorochinskii et al., 2011). In addition, the tissue culturing process frequently results in somaclonal variation, due to an increase in mutation rate and changes in ploidy (Larkin & Scowcroft, 1981; Swedlund & Vasil, 1985), and can account for many of the unintended effects seen in genetically modified plants (Filipecki & Malepszy, 2006).…”

Section: Introductionmentioning

confidence: 99%

Mowing frequency and performance of genetically modified St. Augustinegrass and standard commercial cultivars

Buhlman

Freshour²,

Harriman³

et al. 2022

Intl Turfgrass Soc Res J

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Mowing is a key input when maintaining a turfgrass lawn but has an environmental impact and represents a repetitive expense. In an effort to reduce canopy growth rate and to improve weed control, genetically modified St. Augustinegrasses [Stenotaphrum secundatum (Walter) Kuntze] derived from the cultivar 'Floratam' are now in production and characterized by reduced levels of t gibberellic acid by insertion of the spinach (Spinacia oleracea L.) gibberellic acid 2-oxidase gene and glyphosate tolerance due to the insertion of 5-enolpyruvylshikimate-3-phosphate synthase gene from Arabidopsis thaliana (L.) Heynh. The objective of this study was to determine if eight genetically modified St. Augustinegrass entries required less frequent mowing than two standard commercial cultivars, Floratam and '615866' (Seville™), while maintaining turfgrass quality (TQ) comparable to the standards. This study was planted in 2017 as a randomized complete block design at two Florida locations. Mowing frequency, TQ, density, and color were recorded from 2018 to 2019.Gray leaf spot severity was rated when present. Overall, the genetically modified St. Augustinegrass entries required less frequent mowing than standard cultivars, with PV-A and PV-B requiring less frequent mowing than Floratam across all seasons at both locations. Of these two, PV-A showed TQ comparable to Floratam at Fort Lauderdale Research and Education Center for the entirety of the trial, and at Plant Science Research and Education Unit for 7/10 seasons. For end-users, the availability of a high performing turfgrass with reduced mowing requirements can lead to a decreased maintenance cost and a reduced environmental impact.

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“…However, unintended effects can be resulted from the random insertion of exogenous DNA fragments into the plant genome because the insertion event may alter the expression of certain plant intrinsic genes by disruption, silencing, activation, or modification. Although these unintended effects may be deleterious, beneficial, or neutral with respect to plant health or food safety, the public has shown considerable concern regarding potential hazardous effects of GM plants to agro system and human consumer 2 , 3 . The large-scale commercial release of GM crops has sparked intense debates worldwide 3 , 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Although these unintended effects may be deleterious, beneficial, or neutral with respect to plant health or food safety, the public has shown considerable concern regarding potential hazardous effects of GM plants to agro system and human consumer 2 , 3 . The large-scale commercial release of GM crops has sparked intense debates worldwide 3 , 4 .…”

Section: Introductionmentioning

confidence: 99%

Differentially Expressed Genes Distributed Over Chromosomes and Implicated in Certain Biological Processes for Site Insertion Genetically Modified Rice Kemingdao

Zhi¹,

Li²,

Zhao³

et al. 2012

Int. J. Biol. Sci.

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Release of genetically modified (GM) plants has sparked off intensive debates worldwide partly because of concerns about potential adverse unintended effects of GM plants to the agro system and the safety of foods. In this study, with the aim of revealing the molecular basis for unintended effects of a single site insertion GM Kemingdao (KMD) rice transformed with a synthetic cry1Ab gene, and bridging unintended effects of KMD rice through clues of differentially expressed genes, comparative transcriptome analyses were performed for GM KMD rice and its parent rice of Xiushui11 (XS11). The results showed that 680 differentially expressed transcripts were identified from 30-day old seedlings of GM KMD rice. The absolute majority of these changed expression transcripts dispersed and located over all rice chromosomes, and existed physical distance on chromosome from the insertion site, while only two transcripts were found to be differentially expressed within the 21 genes located within 100 kb up and down-stream of the insertion site. Pathway and biology function analyses further revealed that differentially expressed transcripts of KMD rice were involved in certain biological processes, and mainly implicated in two types of pathways. One type was pathways implicated in plant stress/defense responses, which were considerably in coordination with the reported unintended effects of KMD rice, which were more susceptible to rice diseases compared to its parent rice XS11; the other type was pathways associated with amino acids metabolism. With this clue, new unintended effects for changes in amino acids synthesis of KMD rice leaves were successfully revealed. Such that an actual case was firstly provided for identification of unintended effects in GM plants by comparative transciptome analysis.

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Unintended effects of genetic modifications and methods of their analysis in plants

Cited by 9 publications

References 64 publications

The Combination of Quantitative PCR and Western Blot Detecting CP4‐EPSPS Component in Roundup Ready Soy Plant Tissues and Commercial Soy‐Related Foodstuffs

The Combination of Quantitative PCR and Western Blot Detecting CP4‐EPSPS Component in Roundup Ready Soy Plant Tissues and Commercial Soy‐Related Foodstuffs

Mowing frequency and performance of genetically modified St. Augustinegrass and standard commercial cultivars

Differentially Expressed Genes Distributed Over Chromosomes and Implicated in Certain Biological Processes for Site Insertion Genetically Modified Rice Kemingdao

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