Unilateral ureteral obstruction evokes renal tubular apoptosis via the enhanced oxidative stress and endoplasmic reticulum stress in the rat

Yeh, Chung Hsin; Chiang, Han Sun; Lai, Ting Yu; Chien, Chiang Ting

doi:10.1002/nau.20855

Cited by 54 publications

(47 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was also consistent with other studies. 46,51,62,63 We might draw a stable conclusion that the increased expression of PAX2 was associated with cell apoptosis, especially with RTEC apoptosis, but the decreased expression of PAX2 did not take part in the progression of RIF. However, the exact mechanism is complicated and not well elucidated at the moment and more studies are needed in the future.…”

Section: Mrna Expression Of Pax2mentioning

confidence: 97%

“…We speculated that the mechanism was as follows: The generation of ROS was increased in the renal tissue of UUO rats. 51,52 The increased ROS might upregulate the gene expression of PAX2, 49,50 which regulated the expression of TGF-β1. 53,54 The disorder of TGF-β1 might induce the expressions of α-SMA, Col-IV, and FN, [21][22][23] and the increased TGF-β1 upregulated the expression of caspase-3.…”

Section: Mrna Expression Of Pax2mentioning

confidence: 99%

See 1 more Smart Citation

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

et al. 2012

View full text Add to dashboard Cite

Renal interstitial fibrosis (RIF) is the final common pathway for chronic kidney disease. Cell apoptosis is a critical detrimental event that leads to renal fibrosis. Paired box 2 (PAX2) plays a major role in the development of the kidney. This study was performed to investigate whether PAX2 was associated with cell apoptosis in the progression of RIF in unilateral ureteral obstruction (UUO) rats. Eighty Wistar male rats were divided into two groups randomly: sham operation group (SHO) and model group subjected to UUO (GU), n = 40, respectively. The model was established by left ureteral ligation. Renal tissues were collected 14 and 28 days after surgery. Protein expressions of PAX2, transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), collagen-IV (Col-IV), fibronectin (FN), and caspase-3 were detected using immunohistochemical analysis; mRNA expression of PAX2 in renal tissue was detected by real-time reverse transcription polymerase chain reaction; and RIF index and cell apoptosis index in renal interstitium were also calculated. When compared with those in the SHO group, expressions of PAX2 (protein and mRNA) were markedly increased in the GU group (each p < 0.01). Protein expressions of TGF-β1, α-SMA, Col-IV, FN, and caspase-3 and RIF index and cell apoptosis index in the GU group were remarkably increased when compared with those in the SHO group (each p < 0.01). The protein expression of PAX2 was positively correlated with the protein expressions of TGF-β1, α-SMA, Col-IV, FN, and caspase-3 and with RIF index and cell apoptosis index (all p < 0.01). The apoptotic cell in our observation was mainly derived from renal tubular epithelial cells. In conclusion, the increased expression of PAX2 is associated with cell apoptosis in the progression of RIF in UUO rats, suggesting that PAX2 is a potentially therapeutic target for prevention of RIF.

show abstract

Section: Mrna Expression Of Pax2mentioning

confidence: 97%

Section: Mrna Expression Of Pax2mentioning

confidence: 99%

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Increased hydrostatic pressure causes damage to the tubulointerstitial compartment of the kidney. 3 Apoptosis in tubular cells, capillary rarefaction, and interstitial cell inflammatory infiltration can be observed. The ensuing progressive fibrosis results in loss of parenchyma.…”

Section: Introductionmentioning

confidence: 99%

Alleviation of Kidney Damage Induced by Unilateral Ureter Obstruction in Rats byRhodiola rosea

Üyetürk

Terzi

Kemahlı

et al. 2013

Journal of Endourology

View full text Add to dashboard Cite

Purpose: To evaluate the efficacy of Rhodiola rosea extract in terms of alleviating the renal damage induced by unilateral ureter obstruction (UUO) in rats. Material and Methods: Thirty Wistar albino male rats were divided into five groups: (I) Control, (II) UUO 7 days, (III) UUO 7 days + extract,(IV) UUO 14 days, and (V) UUO 14 days + extract. Seven or 14 days after the initiation of the experimental procedure, the left kidneys of rats in all five groups were removed for histological examination, and their blood was drawn for biochemical measurements. Result: Median malondialdehyde (MDA) and glutathione peroxidase (GPx) levels were, respectively, 39.4 (5.04) nmol/mL and 25.8 (8.01) nmol/minute/mL in group I, 77.9 (12.38) nmol/mL and 5.8 (1.95) nmol/minute/mL in group II, 48.7 (12.1) nmol/mL and 9.1 (2.3) nmol/minute/mL in group III, 58.5 (23.83) nmol/mL and 8.4 (2.1) nmol/minute/mL in group IV, and 44.8 (4.97) nmol/mL and 13.8 (3.73) nmol/minute/mL in group V. There was a statistically significant difference among the groups in terms of MDA and GPx levels ( p < 0.05 for both). The median numbers of apoptotic cells were 1 (1), 8 (2.25), 3 (1.25), 23.5 (9), and 7 (I) in groups I, II, III, IV, and V, respectively. There was a statistically siginificant difference among the groups in terms of apoptotic cell number ( p < 0.05). Conclusion: R. rosea extract was shown to alleviate the renal damage induced by UUO through its antioxidant effects. The mechanism by which R. rosea extract causes these effects merits further investigation.

show abstract

“…4) and are regulated by NFE2L2, which is activated upon exposure to ROS to effect transcriptional changes in response to oxidative stress (21). Oxidative stress has been implicated in the pathogenesis of UPJO and animal models previously (22)(23)(24). A total of 24 statistically significant proteins were associated with oxidative stress, of which 13 are represented in the putative biomarker panel (Table III).…”

Section: Discussionmentioning

confidence: 99%

Urinary Proteomics Yield Pathological Insights for Ureteropelvic Junction Obstruction

Froehlich

Kostel

Cho

et al. 2016

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Prenatal hydronephrosis is a common condition that may spontaneously resolve after birth. However, this condition can result in renal damage and requires surgical correction in a number of cases. Preventing renal damage is paramount, but existing diagnostic technology is invasive, exposes infants to radiation, is costly, and is often indeterminate. A better understanding of the pathophysiology of renal obstruction as reflected in the urinary proteome may provide new insights into the disease that could potentially alter the clinical management of hydronephrosis. We performed a quantitative proteomics study of urine that was surgically obtained from eight clinically significant, unilaterally obstructed infants versus eight healthy controls, with the goal of identifying quantitatively varying proteins and the biological networks associated with them. Notably, urine was obtained from both the obstructed kidney and the bladder. Over 1100 proteins were identified, and a total of 76 quantitatively varying proteins were identified. Proteins involved in oxidative stress, inflammation, and renal disease pathways showed the most significant abundance differences. This study gives a deeper understanding of the critical proteomic changes associated with renal obstruction and represents the deepest proteomic profile of renal obstruction to date.

show abstract

Unilateral ureteral obstruction evokes renal tubular apoptosis via the enhanced oxidative stress and endoplasmic reticulum stress in the rat

Abstract: Our data suggest that renal tubular apoptosis induced by oxidative stress and ER stress occurred in the UUO kidney.

Cited by 54 publications

References 31 publications

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

Association of PAX2 with Cell Apoptosis in Unilateral Ureteral Obstruction Rats

Alleviation of Kidney Damage Induced by Unilateral Ureter Obstruction in Rats byRhodiola rosea

Urinary Proteomics Yield Pathological Insights for Ureteropelvic Junction Obstruction

Contact Info

Product

Resources

About