Unifying concepts in methanogenic, aerobic, and anammox sludge granulation

Mills, Simon; Trego, Anna Christine; Prevedello, Marco; Vrieze, Jo De; O’Flaherty, Vincent; Lens, P.N.L.; Collins, Gavin

doi:10.1016/j.ese.2023.100310

Cited by 9 publications

(1 citation statement)

References 169 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, granular biofilms are widely applied in complex microbial community-catalyzed environmental biotechnology, including in AD of wastes, nutrient removal from wastewater, and waste bioproduction technologies (Beun et al., 1999 ; Carvajal-Arroyo et al., 2019 ; Imajo et al., 2004 ; Mills et al., 2024 ). Critically, the ease of retaining these millimeter-scale, fast-settling, spherical biofilms allows for achieving higher biomass densities, and consequently improves process rates up to an order-of-magnitude compared to planktonic systems (Carvajal-Arroyo et al., 2019 ; Mills et al., 2024 ). Developing synthetic granular biofilm consortia could therefore potentially improve both process rates and ecological stability, key properties for real-world applications.…”

Section: Bottom-up Design Approach To Develop Synthetic Microbial Con...mentioning

confidence: 99%

Top-down and bottom-up microbiome engineering approaches to enable biomanufacturing from waste biomass

Lyu,

Nuhu,

Candry

et al. 2024

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Growing environmental concerns and the need to adopt a circular economy have highlighted the importance of waste valorization for resource recovery. Microbial consortia-enabled biotechnologies have made significant developments in the biomanufacturing of valuable resources from waste biomass that serve as suitable alternatives to petrochemical-derived products. These microbial consortia are designed following a top-down or bottom-up engineering approach. The top-down approach is a classical method that uses environmental variables to selectively steer an existing microbial consortium to achieve a target function. While high-throughput sequencing has enabled microbial community characterization, the major challenge is to disentangle complex microbial interactions and manipulate the structure and function accordingly. Microbial consortia design through a bottom-up approach uses prior knowledge of the metabolic pathway and possible interactions among consortium partners to design and engineer synthetic microbial consortia. This strategy offers some control over the composition and function of the consortium for targeted bioprocesses, but challenges remain in optimal assembly methods and long-term stability. In this review, we present the recent advancements, challenges, and opportunities for further improvement using top-down and bottom-up approaches for microbiome engineering. As the bottom-up approach is relatively a new concept for waste valorization, this review explores the assembly and design of synthetic microbial consortia, ecological engineering principles to optimize microbial consortia, and metabolic engineering approaches for efficient conversion. Integration of top-down and bottom-up approaches along with developments in metabolic modeling to predict and optimize consortia function are also highlighted.

show abstract

Section: Bottom-up Design Approach To Develop Synthetic Microbial Con...mentioning

confidence: 99%

Top-down and bottom-up microbiome engineering approaches to enable biomanufacturing from waste biomass

Lyu,

Nuhu,

Candry

et al. 2024

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

show abstract

Mechanisms of microbial co-aggregation in mixed anaerobic cultures

Doloman,

Sousa

2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Co-aggregation of anaerobic microorganisms into suspended microbial biofilms (aggregates) serves ecological and biotechnological functions. Tightly packed aggregates of metabolically interdependent bacteria and archaea play key roles in cycling of carbon and nitrogen. Additionally, in biotechnological applications, such as wastewater treatment, microbial aggregates provide a complete metabolic network to convert complex organic material. Currently, experimental data explaining the mechanisms behind microbial co-aggregation in anoxic environments is scarce and scattered across the literature. To what extent does this process resemble co-aggregation in aerobic environments? Does the limited availability of terminal electron acceptors drive mutualistic microbial relationships, contrary to the commensal relationships observed in oxygen-rich environments? And do co-aggregating bacteria and archaea, which depend on each other to harvest the bare minimum Gibbs energy from energy-poor substrates, use similar cellular mechanisms as those used by pathogenic bacteria that form biofilms? Here, we provide an overview of the current understanding of why and how mixed anaerobic microbial communities co-aggregate and discuss potential future scientific advancements that could improve the study of anaerobic suspended aggregates. Key points • Metabolic dependency promotes aggregation of anaerobic bacteria and archaea • Flagella, pili, and adhesins play a role in the formation of anaerobic aggregates • Cyclic di-GMP/AMP signaling may trigger the polysaccharides production in anaerobes

show abstract