“…However, different plant protection products present in spray solutions can increase or reduce their adhesion on targets (BARBOSA et al, 2013) and hence change the degree of difficulty of marker removal. Another uncertain factor is the water volume used to wash targets: 20 mL for coffee leaves (SCUDELER et al, 2004), 40 mL for citrus leaves (PALLADINI et al, 2005), 20 and 30 mL in soybean leaves (PINTO et al, 2007 andPRADO et al, 2015, respectively), 50 mL for artificial target (SASAKI et al, 2013), 100 mL for tomato leaves (ZAMBIANCO, 2013), and 100 and 150 mL for peanut and Brachiaria plantaginea leaves (RODRIGUES-COSTA et al, 2011). In addition to this removal volume variability, there are no studies in the literature demonstrating the efficiency of a single washing for a complete removal of a marker (dye).…”