Uniform Susceptibility of Various Strains of
            <i>Coccidioides immitis</i>
            to Amphotericin B

Collins, Michael; Pappagianis, Demosthenes

doi:10.1128/aac.11.6.1049

Cited by 18 publications

(4 citation statements)

References 23 publications

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“…However, it is unknown at this time whether both species occur together in southern California, as no molecular genetic analyses of samples collected from soil surveys have been done south of the Tehachapi Mountains, where Coccidioides is known to occur 13 . Although characterization of variation among isolates with respect to drug therapy 32 and vaccine development 33,34 has been addressed, interspecific variation should be considered in the development of treatment strategies, as shown in Johannesson et al 35 …”

Section: Variation Among Strainsmentioning

confidence: 99%

The Population Biology of Coccidioides

Barker

Jewell

Kroken

et al. 2007

Annals of the New York Academy of Sciences

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Studies of field- and patient-derived isolates conducted over the past 75 years have provided a general picture of the population structure of Coccidioides, the cause of coccidioidomycosis. Premolecular studies provided a general outline of the geographical range, epidemiology and distribution of the fungus. Recent studies based on molecular markers have demonstrated that the genus is comprised of two genetically diverse, and genetically isolated, species: Coccidioides immitis and C. posadasii. Both species are composed of biogeographically distinct populations. Structure for two of these populations (C. immitis from central California, and C. posadasii from southern Arizona) indicates that frequent genetic recombination occurs within the entire geographic range of each population, even though sex has never been observed in the genus. Outbreaks of coccidioidomycosis are not the result of the spread of a single clonal isolate, but are caused by a diversity of genotypes. Although it is now possible to match patient isolates to populations, the lack of apparent structure within each population and the current paucity of environmental isolates limit map-based epidemiological approaches to understanding outbreaks. Therefore, a comprehensive database comprised of soil-derived isolates from across the biogeographic range of Coccidioides will improve the utility of this approach. Appropriate collection of environmental isolates will assist the investigation of remaining questions regarding the population biology of Coccidioides. The comparative genomics of representative genotypes from both species and all populations of Coccidioides will provide a thorough set of genetic markers in order to resolve the population genetics of this pathogenic fungus.

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Section: Variation Among Strainsmentioning

confidence: 99%

The Population Biology of Coccidioides

Barker

Jewell

Kroken

et al. 2007

Annals of the New York Academy of Sciences

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“…Although the importance of testing the parasitic versus the saprophytic phase of dimorphic fungi has yet to be established, using the former has an inherent appeal. In tests of the susceptibility of C. immitis to amphotericin B, the MICs for the SE phase were approximately 8to 10-fold lower than those for the mycelial phase (2,11). Similarly, although the SE phase of this fungus was found to be very sensitive to the chitin synthase inhibitor polyoxin D, the mycelial phase appeared to be refractory (9).…”

mentioning

confidence: 94%

“…Under the conditions established for in vitro growth (3)(4)(5)(6)13), liquid-phase MIC testing would not be practicable without nephelometric measurement because the culture is typically seeded to a density already detectable with the unaided eye. At least three previous reports made use of the SE phase in determinations of MICs (2,11,17). Other reports of susceptibility testing with C. immitis have been concerned either with the saprophytic phase (7,10,17) or with an endospore inoculum which was allowed to convert to the filamentous stage during the incubation period (12,14).…”

mentioning

confidence: 99%

Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis

Hector¹,

Zimmer²,

Pappagianis³

1988

J Clin Microbiol

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A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37°C with shaking for 96 h, after which the control wells had visible turbidity and endpoints were discernible. Microscopic examination revealed that both control and treatment wells maintained cells predominantly in the spherule-endospore phase of growth.

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“…1:8 (4, 10, 12, 18). Amphotericin B (5,7,14) and ketoconazole (2,14,18,19) were the drugs of choice, but at present they are no longer FIG. 3.…”

mentioning

confidence: 99%

Traveller's coccidioidomycosis: case report of pulmonary infection diagnosed in Israel

et al. 1992

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A 60-year-old temporary Israeli resident travelled to Arizona, developed an influenzalike infection, and returned with a space-occupying lesion in the lung. Since the patient was a heavy smoker, lung cancer was suspected and he was operated on. A granuloma was reported on frozen sections, and Coccidioides immitis was revealed on stained preparations and by microbiological investigation. Coccidioidomycosis is unusual in Israel; therefore, it is important to be aware of this mycosis in patients who have a history of recent visits to areas of endemicity in North America, Central America, and South America.

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Uniform Susceptibility of Various Strains of Coccidioides immitis to Amphotericin B

Cited by 18 publications

References 23 publications

The Population Biology of Coccidioides

The Population Biology of Coccidioides

Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis

Traveller's coccidioidomycosis: case report of pulmonary infection diagnosed in Israel

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