Uniform-over-size approximation of the internal fields for scatterers with low refractive-index contrast

Inzhevatkin, Konstantin G.; Yurkin, Maxim A.

doi:10.1016/j.jqsrt.2021.107965

Cited by 7 publications

(4 citation statements)

References 47 publications

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“…Biological cells usually have the relative refractive index (RI) close to 1 (e.g., 1.01-1.05) and lower scattering intensity than polystyrene beads with comparable size. Side scattering (which is measured by our prototype) is several orders of magnitude lower than forward scattering, especially for large optically soft particles with homogeneous internal structure (10,11).…”

Section: Cell Culture Testingmentioning

confidence: 85%

Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype

Litunenko,

Moskalensky

2023

Preprint

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Cell cultures are widely used in scientific research, biomedicine and industry. When culturing, it is important to maintain certain conditions, including the concentration of cells. Monitoring of the culture growth and cell counting is an urgent task for the optimization of technological processes. Most existing methods require sampling from a culture flask. This procedure is time-consuming and associated with the risks of contamination. We present a device able to monitor the growth of cells number in a suspension non-invasively. The device uses a laser beam that pass through the culture flask and measures the intensity of scattered light as a function of coordinate along the beam. This optical scheme allows one to obtain accurate results for both high- and low-scattering samples. We constructed the wireless portable prototype for monitoring of cell culture growth directly in the incubator and demonstrated the applicability of the device for Jurkat cells and e.coli bacteria.

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Section: Cell Culture Testingmentioning

confidence: 85%

Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype

Litunenko,

Moskalensky

2023

Preprint

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“…In the future, the DDA should be improved, in particular, it will therefore be necessary to focus on its shortcomings, particularly on the resolution of the system of linear equations, for example with a preconditioner to accelerate the iterative method [91,92] or by improving the initial estimate of the iterative method [93,94]. This would make it possible to deal with larger objects.…”

Section: Discussionmentioning

confidence: 99%

The Discrete Dipole Approximation: A Review

Chaumet

2022

Mathematics

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There are many methods for rigorously calculating electromagnetic diffraction by objects of arbitrary shape and permittivity. In this article, we will detail the discrete dipole approximation (DDA) which belongs to the class of volume integral methods. Starting from Maxwell’s equations, we will first present the principle of DDA as well as its theoretical and numerical aspects. Then, we will discuss the many developments that this method has undergone over time and the numerous applications that have been developed to transform DDA in a very versatile method. We conclude with a discussion of the strengths and weaknesses of the DDA and a description of the freely available DDA-based electromagnetic diffraction codes.

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Section: Cell Culture Testingmentioning

confidence: 99%

Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype

Litunenko,

Moskalensky

2023

Journal of Biophotonics

View full text Add to dashboard Cite

Cell cultures are widely used in scientific research, biomedicine and industry. When culturing, it is important to maintain certain conditions, including the concentration of cells. Monitoring of the culture growth and cell counting is an urgent task for the optimization of technological processes. Most existing methods require sampling from a culture flask. This procedure is time‐consuming and associated with the risks of contamination. We present a device able to monitor the growth of cells number in a suspension non‐invasively. The device uses a laser beam that pass through the culture flask and measures the intensity of scattered light as a function of coordinate along the beam. This optical scheme allows one to obtain accurate results for both high‐ and low‐scattering samples. We constructed the wireless portable prototype for monitoring of cell culture growth directly in the incubator and demonstrated the applicability of the device for Jurkat cells and e.coli bacteria.This article is protected by copyright. All rights reserved.

show abstract

Uniform-over-size approximation of the internal fields for scatterers with low refractive-index contrast

Cited by 7 publications

References 47 publications

Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype

Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype

The Discrete Dipole Approximation: A Review

Wireless monitoring of cell cultures based on light scattering: A novel optical scheme and portable prototype

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