Unified quantitative model of AMPA receptor trafficking at synapses

Abstract

Trafficking of AMPA receptors (AMPARs) plays a key role in synaptic transmission. However, a general framework integrating the two major mechanisms regulating AMPAR delivery at postsynapses (i.e., surface diffusion and internal recycling) is lacking. To this aim, we built a model based on numerical trajectories of individual AMPARs, including free diffusion in the extrasynaptic space, confinement in the synapse, and trapping at the postsynaptic density (PSD) through reversible interactions with scaffold proteins. The AMPAR/scaffold kinetic rates were adjusted by comparing computer simulations to single-particle tracking and fluorescence recovery after photobleaching experiments in primary neurons, in different conditions of synapse density and maturation. The model predicts that the steady-state AMPAR number at synapses is bidirectionally controlled by AMPAR/scaffold binding affinity and PSD size. To reveal the impact of recycling processes in basal conditions and upon synaptic potentiation or depression, spatially and temporally defined exocytic and endocytic events were introduced. The model predicts that local recycling of AMPARs close to the PSD, coupled to short-range surface diffusion, provides rapid control of AMPAR number at synapses. In contrast, because of long-range diffusion limitations, extrasynaptic recycling is intrinsically slower and less synapse-specific. Thus, by discriminating the relative contributions of AMPAR diffusion, trapping, and recycling events on spatial and temporal bases, this model provides unique insights on the dynamic regulation of synaptic strength.C ontrolling the number of AMPA-type glutamate receptors (AMPARs) at excitatory synapses is of fundamental importance in synaptic transmission (1). AMPARs are anchored at the postsynaptic density (PSD) via specific interactions with scaffold molecules, but can dynamically exchange between intracellular and extrasynaptic membrane compartments. This turnover involves two major mechanisms: endo/exocytic recycling and surface diffusion (2).A number of studies have demonstrated the importance of AMPAR recycling in synaptic plasticity. Synaptic potentiation induces AMPAR exocytosis, whereas disrupting basal exocytosis leads to a run-down of AMPAR-dependent synaptic transmission and reduces long-term potentiation (LTP) (3-8). Inversely, inhibition of basal endocytosis gradually increases AMPAR excitatory postsynaptic currents (EPSCs), and occludes long-term depression (LTD) (1, 9). Furthermore, an endocytic zone (EZ) located near the PSD and responsible for local AMPAR recycling is essential for regulating synaptic transmission (10-12). Despite these advances, the exact locations and kinetics of AMPAR exocytosis and endocytosis, both in basal conditions and in response to LTP and LTD stimuli, respectively, are still unclear (13).The other mechanism controlling AMPAR trafficking at synapses is surface diffusion (14). Fluorescence recovery after photobleaching (FRAP) and single-particle tracking (SPT) experiments have shown that AMPARs diff...

ERK regulation of phosphodiesterase 4 enhances dopamine-stimulated AMPA receptor membrane insertion

“…Several computational models have explored the underlying signaling that controls AMPAR trafficking (16)(17)(18)(19)(20). Nakano et al specifically explored the contribution of dopamine signaling to AMPAR trafficking in striatal neurons (18).…”

Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors

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Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation

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