Unidirectional migration of single smooth muscle cells under the synergetic effects of gradient swelling cue and parallel groove patterns

Han, Lulu; Mao, Zhengwei; Wu, Jindan; Guo, Yang; Ren, Tanchen; Gao, Changyou

doi:10.1016/j.colsurfb.2013.05.011

Cited by 25 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell migration can occur two-dimensionally (2D) and three-dimensionally (3D). The investigation of cell migration in vitro is predominantly carried out on a planar surface, revealing the influence of stiffness [ 8 ], topology [ 9 ] and chemical structure [ 10 ] of the matrixes. Although the 2D migration results are instructive to guide the design of biomaterials for some practical applications, the 3D migration can better mimic the cell behaviors during the process of tissue regeneration in vivo .…”

Section: Introductionmentioning

confidence: 99%

Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ

Dai

Shen

et al. 2017

Regenerative Biomaterials

Self Cite

View full text Add to dashboard Cite

Cell migration in scaffolds plays a crucial role in tissue regeneration, which can better mimic cell behaviors in vivo. In this study, a novel model has been proposed on controlling 3D cell migration in porous collagen-chitosan scaffolds with various pore structures under the stimulation of inflammatory cells to mimic the angiogenesis process. Endothelial cells (ECs) cultured atop the scaffolds in the Transwell molds which were placed into a well of a 24-well culture plate were promoted to migrate into the scaffolds by chemoattractants such as vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-α) secreted by the pro-inflammatory macrophages incubated in the well culture plate. The phenotype of macrophages was mediated by 50 ng/ml interferon-gamma (IFN-γ) and different concentrations of lipopolysaccharide (LPS, 150–300 ng/ml). The cell migration depth had a positive correlation with LPS concentration, and thereby the TNF-α concentration. The ECs migrated easier to a deeper zone of the scaffolds prepared at − 10ºC (187 μm in pore diameter) than that at − 20ºC (108 μm in pore diameter) as well. The method provides a useful strategy to study the 3D cell migration, and is helpful to reveal the vascularization process during wound healing in the long run.

show abstract

Section: Introductionmentioning

confidence: 99%

Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ

Dai

Shen

et al. 2017

Regenerative Biomaterials

Self Cite

View full text Add to dashboard Cite

show abstract

“…Besides surface topography, stimulations such as chemical gradient and fluid flow also play roles in cell migration. Han et al [45] assembled poly(sodium 4-styrenesulfonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers on PDMS membrane with linear grooves and ridges and treated the surface with a gradient solution of 3-5 M NaCl. The synergetic effects of the surface topography and swelling gradient can effectively guide the unidirectional migration of single smooth muscle cells.…”

Section: Manipulation Of Cell Migration On Surfacesmentioning

confidence: 99%

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

Zheng

Jiang

2014

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

“…6) [230–231]. In different studies, both SMCs and ECs, were observed to migrate directed along the groove direction [12–13 204,214,232–233]. It has been reported for some cell types that their migration velocity on microstructured surfaces increased compared to flat surfaces [212,234–237].…”

Section: Reviewmentioning

confidence: 99%

“…It has been reported for some cell types that their migration velocity on microstructured surfaces increased compared to flat surfaces [212,234–237]. Nevertheless, there is still no clear consensus about the effect of surface topography on SMCs and ECs migration velocity [32,214,232–233 238]. A possible explanation to the differences in migration velocity on topographies would be the dynamicity of FAs.…”

Section: Reviewmentioning

confidence: 99%

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

Greiner¹,

Sales²,

Chen³

et al. 2016

Beilstein J. Nanotechnol.

View full text Add to dashboard Cite

The extracellular environment of vascular cells in vivo is complex in its chemical composition, physical properties, and architecture. Consequently, it has been a great challenge to study vascular cell responses in vitro, either to understand their interaction with their native environment or to investigate their interaction with artificial structures such as implant surfaces. New procedures and techniques from materials science to fabricate bio-scaffolds and surfaces have enabled novel studies of vascular cell responses under well-defined, controllable culture conditions. These advancements are paving the way for a deeper understanding of vascular cell biology and materials–cell interaction. Here, we review previous work focusing on the interaction of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) with materials having micro- and nanostructured surfaces. We summarize fabrication techniques for surface topographies, materials, geometries, biochemical functionalization, and mechanical properties of such materials. Furthermore, various studies on vascular cell behavior and their biological responses to micro- and nanostructured surfaces are reviewed. Emphasis is given to studies of cell morphology and motility, cell proliferation, the cytoskeleton and cell-matrix adhesions, and signal transduction pathways of vascular cells. We finalize with a short outlook on potential interesting future studies.

show abstract

Unidirectional migration of single smooth muscle cells under the synergetic effects of gradient swelling cue and parallel groove patterns

Cited by 25 publications

References 34 publications

Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ

Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

Contact Info

Product

Resources

About