Uniaxially crumpled graphene as a platform for guided myotube formation

Kim, Jung-Hoon; Leem, Juyoung; Kim, Hong Nam; Kang, Pilgyu; Choi, Jonghyun; Haque, Farhadul; Kang, Daeshik; Nam, SungWoo

doi:10.1038/s41378-019-0098-6

Cited by 30 publications

(26 citation statements)

References 54 publications

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“…For example, the introduction of GO in hydrogel is used to increase the surface roughness (Zhou et al, 2018). Further, compressive strain-induced deformation of graphene substrates has been employed to form crumpled folds and cause the alignment and elongation of myoblasts (Kim et al, 2019). Nanotopographies can be added to surfaces of GBMcontaining scaffolds also through laser printing by exploiting light absorption properties of graphene (Papi et al, 2016;Palmieri et al, 2017).…”

Section: Gbm Topographies and Electrospun Gbm Fibersmentioning

confidence: 99%

3D Graphene Scaffolds for Skeletal Muscle Regeneration: Future Perspectives

Palmieri

Sciandra²,

Bozzi

et al. 2020

Front. Bioeng. Biotechnol.

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Although skeletal muscle can regenerate after injury, in chronic damages or in traumatic injuries its endogenous self-regeneration is impaired. Consequently, tissue engineering approaches are promising tools for improving skeletal muscle cells proliferation and engraftment. In the last decade, graphene and its derivates are being explored as novel biomaterials for scaffolds production for skeletal muscle repair. This review describes 3D graphene-based materials that are currently used to generate complex structures able not only to guide cell alignment and fusion but also to stimulate muscle contraction thanks to their electrical conductivity. Graphene is an allotrope of carbon that has indeed unique mechanical, electrical and surface properties and has been functionalized to interact with a wide range of synthetic and natural polymers resembling native musculoskeletal tissue. More importantly, graphene can stimulate stem cell differentiation and has been studied for cardiac, neuronal, bone, skin, adipose, and cartilage tissue regeneration. Here we recapitulate recent findings on 3D scaffolds for skeletal muscle repairing and give some hints for future research in multifunctional graphene implants.

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Section: Gbm Topographies and Electrospun Gbm Fibersmentioning

confidence: 99%

3D Graphene Scaffolds for Skeletal Muscle Regeneration: Future Perspectives

Palmieri

Sciandra²,

Bozzi

et al. 2020

Front. Bioeng. Biotechnol.

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“…The possibility of forming graphene surfaces with specific morphology has been used in cell culture. Mouse myoblast cells (C2C12) inoculated on uniaxially crumpled graphene, became aligned and elongated at the single-cell level, as well the observation of the differentiation and maturation of myotubes compared to that on flat graphene [38]. In our research, we used the nGO surface, which was formed in the process of self-organisation (during drying), so it did not have an ordered surface, imitating the shape of developing fibres.…”

Section: Discussionmentioning

confidence: 99%

Graphene oxide nanofilm and the addition of l-glutamine can promote development of embryonic muscle cells

et al. 2020

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Background: Formation of muscular pseudo-tissue depends on muscle precursor cells, the extracellular matrix (ECM)-mimicking structure and factors stimulating cell differentiation. These three things cooperate and can create a tissue-like structure, however, their interrelationships are relatively unknown. The objective was to study the interaction between surface properties, culture medium composition and heterogeneous cell culture. We would like to demonstrate that changing the surface properties by coating with graphene oxide nanofilm (nGO) can affect cell behaviour and especially their need for the key amino acid l-glutamine (L-Glu). Results: Chicken embryo muscle cells and their precursors, cultured in vitro, were used as the experimental model. The mesenchymal stem cell, collected from the hind limb of the chicken embryo at day 8 were divided into 4 groups; the control group and groups treated with nGO, L-Glu and nGO supplied with L-Glu (nGOxL-Glu). The roughness of the surface of the plastic plate covered with nGO was much lower than a standard plate. The test of nGO biocompatibility demonstrated that the cells were willing to settle on the nGO without any toxic effects. Moreover, nGO by increasing hydrophilicity and reducing roughness and presumably through chemical bonds available on the GO surface stimulated the colonisation of primary stromal cells that promote embryonic satellite cells. The viability significantly increased in cells cultured on nGOxL-Glu. Observations of cell morphology showed that the most mature state of myogenesis was characteristic for the group nGOxL-Glu. This result was confirmed by increasing the expression of MYF5 genes at mRNA and protein levels. nGO also increased the expression of MYF5 and also very strongly the expression of PAX7 at mRNA and protein levels. However, when analysing the expression of PAX7, a positive link was observed between the nGO surface and the addition of L-Glu. Conclusions: The use of nGO and L-Glu supplement may improve myogenesis and also the myogenic potential of myocytes and their precursors by promoting the formation of satellite cells. Studies have, for the first time, demonstrated positive cooperation between surface properties nGO and L-Glu supplementation to the culture medium regarding the myogenic potential of cells involved in muscle formation.

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“…The possibility of forming graphene surfaces with specific morphology has been used in cell culture. Mouse myoblast cells (C2C12) inoculated on uniaxially crumpled graphene, became aligned and elongated at the single-cell level, as well the observation of the differentiation and maturation of myotubes compared to that on flat graphene [38]. In our research, we used the nGO surface, which was formed in the process of selforganisation (during drying), so it did not have an ordered surface, imitating the shape of developing fibres.…”

Section: Discussionmentioning

confidence: 99%

Graphene oxide nanofilm and the addition of L-glutamine can promote development of embryonic muscle cells

Zielińska‐Górska

Hotowy

Wierzbicki

et al. 2020

Preprint

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Background: Formation of muscular pseudo-tissue depends on muscle precursor cells, the extracellular matrix (ECM)-mimicking structure and factors stimulating cell differentiation. These three things cooperate and can create a tissue-like structure, however, their interrelationships are relatively unknown. The objective was to study the interaction between surface properties, culture medium composition and heterogeneous cell culture. We would like to demonstrate that changing the surface properties by coating with graphene oxide nanofilm (nGO) can affect cell behaviour and especially their need for the key amino acid L-glutamine (L-Glu).Results: Chicken embryo muscle cells and their precursors, cultured in vitro, were used as the experimental model. The mesenchymal stem cell, collected from the hind limb of the chicken embryo at day 8 were divided into 4 groups; the control group and groups treated with nGO, L-Glu and nGO supplied with L-Glu (nGOxL-Glu). The roughness of the surface of the plastic plate covered with nGO was much lower than a standard plate. The test of nGO biocompatibility demonstrated that the cells were willing to settle on the nGO without any toxic effects. Moreover, nGO by increasing hydrophilicity and reducing roughness and presumably through chemical bonds available on the GO surface stimulated the colonisation of primary stromal cells that promote embryonic satellite cells. The viability significantly increased in cells cultured on nGOxL-Glu. Observations of cell morphology showed that the most mature state of myogenesis was characteristic for the group nGOxL-Glu. This result was confirmed by increasing the expression of MYF5 genes at mRNA and protein levels. nGO also increased the expression of MYF5 and also very strongly the expression of PAX7 at mRNA and protein levels. However, when analysing the expression of PAX7, a positive link was observed between the nGO surface and the addition of L-Glu.Conclusions: The use of nGO and L-Glu supplement may improve myogenesis and also the myogenic potential of myocytes and their precursors by promoting the formation of satellite cells. Studies have, for the first time, demonstrated positive cooperation between surface properties nGO and L-Glu supplementation to the culture medium regarding the myogenic potential of cells involved in muscle formation.

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Uniaxially crumpled graphene as a platform for guided myotube formation

Cited by 30 publications

References 54 publications

3D Graphene Scaffolds for Skeletal Muscle Regeneration: Future Perspectives

3D Graphene Scaffolds for Skeletal Muscle Regeneration: Future Perspectives

Graphene oxide nanofilm and the addition of l-glutamine can promote development of embryonic muscle cells

Graphene oxide nanofilm and the addition of L-glutamine can promote development of embryonic muscle cells

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