Unfolding of population structure in Baltic sheep breeds using microsatellite analysis

Tapio, Ilma; Tapio, Miika; Grīslis, Z.; Holm, Lars‐Erik; Jeppsson, Sven; Kantanen, Juha; Miceikienė, Ilona Teodora; Olsaker, I.; Viinalass, H.; Eythórsdóttir, Emma

doi:10.1038/sj.hdy.6800640

Cited by 54 publications

(51 citation statements)

References 43 publications

(30 reference statements)

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“…According to the results of F-statistics [28], 11% of the total genetic variation in the Serbian Tsigai sheep can be explained by type differences. This significant differentiation exceeds that detected among six Spanish sheep breeds (6%, eight markers in common with our study) [2], among seven Baltic breeds (8.8%, 21 common markers) [24] and even that presented for 57 European and Middle Eastern sheep breeds (approximately on average 6%, eight common markers) [15]. Moreover, the Bayesian assignment of animals to the source populations supported the clear-cut differentiation between the two Tsigai types (Fig.…”

Section: Discussionsupporting

confidence: 54%

Genetic differentiation between the Old and New types ofSerbianTsigaisheep

Ćinkulov¹,

Tapio²,

Ozerov³

et al. 2008

Genet. Sel. Evol.

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-Two Tsigai sheep populations exist in Serbia: the Old type, calledČokan, and the New type. It is assumed that the New type results from upgrading Tsigai sheep with exotic genetic material. We investigated genetic diversity and differentiation of these types by analysing 23 autosomal microsatellites. Tests for Hardy-Weinberg proportions, linkage equilibrium between genotypes across loci and the calculation of inbreeding coefficients were performed and the deficiency in the number of alleles within the Tsigai types was examined using a Wilcoxon sign-rank test. The New type displayed a higher level of genetic variability than theČokan in terms of allele numbers, but the New Tsigai showed a pattern of heterozygosity deficiency. The positive f value for theČokan suggests the occurrence of inbreeding in this type. The proportion of linkage disequilibrium was below that expected by chance. Exclusion of two loci in HardyWeinberg disequilibrium did not alter our conclusions based on the entire data set i.e. the two Tsigai types are clearly differentiated and the New Tsigai type has been influenced by crossbreeding. Therefore, theČokan Tsigai should be considered as a distinct endangered breed in the FAO classification.

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Section: Discussionsupporting

confidence: 54%

Genetic differentiation between the Old and New types ofSerbianTsigaisheep

Ćinkulov¹,

Tapio²,

Ozerov³

et al. 2008

Genet. Sel. Evol.

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“…The difference of results regarding the distinctiveness between populations sampled in Bosnia and Herzegovina and in Croatia could be a result of systematic recording and selection programme implemented by the Croatian Agriculture Agency during several decades, complemented with clear geographical boundaries between the populations/breeds in Croatia. As reported for Baltic sheep (Tapio et al, 2005), four populations, sampled as traditional breeds in Bosnia and Herzegovina, do not equate to genetically distinct populations. According to the structure analysis and the N m and F st estimates reported here, the largest influence on other Bosnian populations comes from VLA, with status and distribution similar to DAL in Croatia.…”

Section: Discussionmentioning

confidence: 81%

Genetic diversity and differentiation of 12 eastern Adriatic and western Dinaric native sheep breeds using microsatellites

Šalamon

Gutiérrez-Gil

Arranz

et al. 2014

Animal

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Nuclear genetic diversity and differentiation of 341 sheep belonging to 12 sheep breeds from Croatia and Bosnia and Herzegovina were examined. The aim of the study was to provide the understanding of the genetic structure and variability of the analysed pramenka sheep populations, and to give indications for conservation strategies based on the population diversity and structure information. The genetic variation of the sheep populations, examined at the nuclear level using 27 microsatellite loci, revealed considerable levels of genetic diversity, similar to the diversity found in other European indigenous low-production sheep breeds. Population-specific alleles were detected at most loci and in breeds analysed. The observed heterozygosity ranged from 0.643 (in Lika pramenka) to 0.743 (in Vlasic pramenka), and the expected heterozygosity ranged from 0.646 (in Lika pramenka) to 0.756 (in Dalmatian pramenka). Significant inbreeding coefficients were found for half of the populations studied and ranged from 0.040 (Pag island sheep) to 0.091 (Kupres pramenka). Moderate genetic differentiation was found between the studied sheep populations. The total genetic variability observed between different populations was 5.29%, whereas 94.71% of the variation was found within populations. Cres island sheep, Lika pramenka and Istrian sheep were identified as the most distinct populations, which was confirmed by the factorial analysis of correspondence and supported through a bootstrapping adjustment to correct for the difference in the sample sizes. The population structure analysis distinguished 12 clusters for the 12 sheep breeds analysed. However, the cluster differentiation was low for Dalmatian, Vlasic, Stolac and Krk pramenka. This systematic study identified Lika pramenka and Rab island sheep as those with the lowest diversity, whereas Istrian sheep and Pag island sheep had the highest. Conservation actions are proposed for Istrian, Rab and Cres island sheep, Lika and Kupres pramenka because of high estimated coefficients of inbreeding.

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“…From these considerations and given the importance of the local genetic resources, it is easily understandable the considerable interest given nowadays to genetic diversity studies in domestic animals in general and, recently, in small ruminants (Baumung et al, 2004). Genetic diversity studies in domestic animals aim at evaluating genetic variation within and across breeds, since the breed is the management unit for which factors such as inbreeding are controlled (Tapio et al, 2005). However, the definition of a breed, as applied by FAO, frequently does not reflect the underlying genetic population structure.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers

Tolone

Mastrangelo

Rosa

et al. 2012

Small Ruminant Research

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a b s t r a c tGenetic diversity studies in domestic animals aim at evaluating genetic variation within and across breeds mainly for conservation purposes. In Sicily, dairy sheep production represents an important resource for hilly and mountain areas economy. Their milk is used for the production of traditional raw milk cheeses, sometimes protected designation of origin (PDO) cheeses. In some cases, the quality of these products is linked to a specific breed, i.e. mono-breed labelled cheeses and it is therefore important to be able to distinguish the milk of a breed from that of others, in order to guarantee both the consumer and the breed itself. In order to investigate the genetic structure and to perform an assignment test, a total of 331 individuals (Barbaresca, BAR n = 57, Comisana, COM n = 65, Pinzirita, PIN n = 75, Sarda, SAR n = 64, and Valle del Belice, VDB n = 70) were analysed using a panel of 20 microsatellite markers. A total of 259 alleles were observed with average polymorphic information content equal to 0.76, showing that the microsatellites panel used was highly informative. Estimates of observed heterozygosity ranged from 0.65 in the BAR breed to 0.75 in the COM breed. The low value of genetic differentiation among breeds (F st = 0.049) may indicate that these breeds are little differentiated probably due to common history and breeding practices. The low F is and F it values indicated low level of inbreeding within and among breeds. The unrooted neighbor-joining dendrogram obtained from the Reynold's genetic distances, and factorial correspondence analysis revealed a separation between BAR and the other sheep breeds. Recent migration rates were estimated, showing that four out of the five breeds have not received a significant proportion of migrants. Only for the PIN breed a recent introgression rate from the VDB breed (7.2%) was observed. The Bayesian assignment test showed that BAR and SAR breeds had a more definite genetic structure (proportion of assignment of 92% and 86.6%, respectively), whereas the lowest assignment value was found in the PIN breed (67.1%). Our results indicated high genetic variability, low inbreeding and low genetic differentiation, except for BAR breed, and were in accordance with geographical location, history, and breeding practices. The low robustness of the assignment test makes it unfeasible for traceability purposes, due to the high level of admixture, in particular for COM, PIN and VDB.

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Unfolding of population structure in Baltic sheep breeds using microsatellite analysis

Cited by 54 publications

References 43 publications

Genetic differentiation between the Old and New types ofSerbianTsigaisheep

Genetic differentiation between the Old and New types ofSerbianTsigaisheep

Genetic diversity and differentiation of 12 eastern Adriatic and western Dinaric native sheep breeds using microsatellites

Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers

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