volume 32, issue 40, P10527-10531 1993
DOI: 10.1021/bi00091a001
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David W. Brown, Louis J. Libertini, Christine Suquet, Enoch W. Small, Michael J. Smerdon

Abstract: Nucleosome core particles undergo a conformational change at ionic strengths below 0.2 mM; the fluorescence anisotropy decay of bound ethidium indicates that under these conditions the particle adopts a highly extended structure. We have measured the distribution of UV-induced DNA damage (primarily cyclobutane-pyrimidine dimers) through a process termed photofootprinting. As the core particle is exposed to ionic strengths below 0.2 mM, the photofootprint pattern changes from that observed for native cores, wit…

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