Unexplored Brazilian oceanic island host high salt tolerant biosurfactant-producing bacterial strains

Silva, Fábio Sérgio Paulino da; Pylro, Victor Satler; Fernandes, Péricles Leonardo; Barcelos, Gisele Souza; Kalks, Karlos Henrique Martins; Schaefer, Carlos Ernesto Gonçalves Reynaud; Tótola, Marcos Rogério

doi:10.1007/s00792-015-0740-7

Cited by 18 publications

(11 citation statements)

References 37 publications

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“…Most of these isolates have been previously reported in marine or oceanic environments and other highly saline environments (Claverías et al 2015 ; da Silva et al 2015 ). Based on the conditions used for recovering these microorganisms, the majority of our isolates are heterotrophic, mesophilic, aerobic or facultative aerobes that are halophilic or halotolerant organisms that can cope with salt concentrations ranging from 0 to 8% NaCl (w/v).…”

Section: Discussionmentioning

confidence: 94%

“…Halophiles have attracted the interests of researchers because of their adaptability to a wide range of salinities as well as their potentially promising applications. They are sources of compatible solutes, stable enzymes (DNAses, lipases, amylases and proteases), bacteriorhodopsin, polymers, β-carotene and other organic substances of interest (Chen et al 2010 ; Oren 2010 ; Braña et al 2015 ; da Silva et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Microbial diversity of saline environments: searching for cytotoxic activities

et al. 2017

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In order to select halophilic microorganisms as a source of compounds with cytotoxic activities, a total of 135 bacterial strains were isolated from water and sediment samples collected from the Zipaquirá salt mine in the Colombian Andes. We determined the cytotoxic effects of 100 crude extracts from 54 selected organisms on the adherent murine mammary cell carcinoma 4T1 and human mammary adenocarcinoma MCF-7 cell lines. These extracts were obtained from strains of Isoptericola, Ornithinimicrobium, Janibacter, Nesterenkonia, Alkalibacterium, Bacillus, Halomonas, Chromohalobacter, Shewanella, Salipiger, Martellela, Oceanibaculum, Caenispirillum and Labrenzia. The extracts of 23 strains showed an IC50 of less than 100 μg mL−1. They were subsequently analyzed by LC/MS allowing dereplication of 20 compounds. The cytotoxic effect was related to a complex mixture of diketopiperazines present in many of the extracts analyzed. The greatest cytotoxic activity against both of the evaluated cell lines was obtained from the chloroform extract of Labrenzia aggregata USBA 371 which had an IC50 < 6 μg mL−1. Other extracts with high levels of cytotoxic activity were obtained from Bacillus sp. (IC50 < 50 μg mL−1) which contained several compounds such as macrolactin L and A, 7-O-succinoylmacrolactin F and iturin. Shewanella chilikensis USBA 344 also showed high levels of cytotoxic activity against both cell lines in the crude extract: an IC50 < 15 μg mL−1 against the 4T1 cell line and an IC50 < 68 μg mL−1 against the MCF-7 cell line. Nesterenkonia sandarakina CG 35, which has an IC50 of 118 µg mL−1 against 4T1, is a producer of diketopiperazines and 1-acetyl-β-carboline. Also, Ornithinimicrobium kibberense CG 24, which has IC50 < 50 μg mL−1, was a producer of diketopiperazines and lagunamycin. Our study demonstrates that these saline environments are habitats of halophilic and halotolerant bacteria that have previously unreported cytotoxic activity.Electronic supplementary materialThe online version of this article (10.1186/s13568-017-0527-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Microbial diversity of saline environments: searching for cytotoxic activities

et al. 2017

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“…Several studies have reported Actinobacteria as a good option for removing recalcitrant hydrocarbon, since they are known for the production of extracellular enzymes that degrade a wide range of complex hydrocarbons. Also, many species of Actinobacteria are able to produce biosurfactants that enhance hydrocarbons solubility and bioavailability ( Pizzul, Del Pilar Castillo & Stenström, 2007 ; Kim & Crowley, 2007 ; Balachandran et al, 2012 ; Da Silva et al, 2015 ). The Actinobacteria phylum is recognized as the main alkane degrader in polar soils ( Aislabie, Saul & Foght, 2006 ), besides producing multiple types of antifungals, antivirals, antibiotics, immunosuppressives, anti-hypertensives and antitumorals ( Benedict, 1953 ; Ōmura et al, 2001 ; Khan et al, 2011 ; De Lima Procópio et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Responses of microbial community from tropical pristine coastal soil to crude oil contamination

Morais

Pylro

Clark

et al. 2016

PeerJ

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Brazilian offshore crude oil exploration has increased after the discovery of new reservoirs in the region known as pré-sal, in a depth of 7.000 m under the water surface. Oceanic islands near these areas represent sensitive environments, where changes in microbial communities due oil contamination could stand for the loss of metabolic functions, with catastrophic effects to the soil services provided from these locations. This work aimed to evaluate the effect of petroleum contamination on microbial community shifts (Archaea, Bacteria and Fungi) from Trindade Island coastal soils. Microcosms were assembled and divided in two treatments, control and contaminated (weathered crude oil at the concentration of 30 g kg−1), in triplicate. Soils were incubated for 38 days, with CO2 measurements every four hours. After incubation, the total DNA was extracted, purified and submitted for target sequencing of 16S rDNA, for Bacteria and Archaea domains and Fungal ITS1 region, using the Illumina MiSeq platform. Three days after contamination, the CO2 emission rate peaked at more than 20 × the control and the emissions remained higher during the whole incubation period. Microbial alpha-diversity was reduced for contaminated-samples. Fungal relative abundance of contaminated samples was reduced to almost 40% of the total observed species. Taxonomy comparisons showed rise of the Actinobacteria phylum, shifts in several Proteobacteria classes and reduction of the Archaea class Nitrososphaerales. This is the first effort in acquiring knowledge concerning the effect of crude oil contamination in soils of a Brazilian oceanic island. This information is important to guide any future bioremediation strategy that can be required.

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“…As being previously observed by other authors, we could not observe a close link in these tests. For example, da Silva et al [ 34 ] isolated strains with hemolytic activity; however, only 33 % were confirmed as biosurfactant producers via the oil-spreading method.…”

Section: Discussionmentioning

confidence: 99%

Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production

et al. 2015

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BackgroundBiosurfactants are surface-active biomolecules with great applicability in the food, pharmaceutical and oil industries. Endospore-forming bacteria, which survive for long periods in harsh environments, are described as biosurfactant producers. Although the ubiquity of endospore-forming bacteria in saline and hypersaline environments is well known, studies on the diversity of the endospore-forming and biosurfactant-producing bacterial genera/species in these habitats are underrepresented.MethodsIn this study, the structure of endospore-forming bacterial communities in sediment/mud samples from Vermelha Lagoon, Massambaba, Dois Rios and Abraão Beaches (saline environments), as well as the Praia Seca salterns (hypersaline environments) was determined via denaturing gradient gel electrophoresis. Bacterial strains were isolated from these environmental samples and further identified using 16S rRNA gene sequencing. Strains presenting emulsification values higher than 30 % were grouped via BOX-PCR, and the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20 % NaCl to test their emulsifying activities in these extreme conditions. Mass spectrometry analysis was used to demonstrate the presence of surfactin.ResultsA diverse endospore-forming bacterial community was observed in all environments. The 110 bacterial strains isolated from these environmental samples were molecularly identified as belonging to the genera Bacillus, Thalassobacillus, Halobacillus, Paenibacillus, Fictibacillus and Paenisporosarcina. Fifty-two strains showed emulsification values of at least 30%, and they were grouped into18 BOX groups. The stability of the emulsification values varied when the culture supernatants of representative strains were subjected to high temperatures and to the presence of up to 20% NaCl. The presence of surfactin was demonstrated in one of the most promising strains.ConclusionThe environments studied can harbor endospore-forming bacteria capable of producing biosurfactants with biotechnological applications. Various endospore-forming bacterial genera/species are presented for the first time as biosurfactant producers.

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Unexplored Brazilian oceanic island host high salt tolerant biosurfactant-producing bacterial strains

Cited by 18 publications

References 37 publications

Microbial diversity of saline environments: searching for cytotoxic activities

Microbial diversity of saline environments: searching for cytotoxic activities

Responses of microbial community from tropical pristine coastal soil to crude oil contamination

Exploiting the aerobic endospore-forming bacterial diversity in saline and hypersaline environments for biosurfactant production

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