Unexplained variations in life span of the Oregon-R strain of Drosophila melanogaster over a four-year period

Lints, F.A.; Lints, C.V.; Bullens, Pol; Bourgois, M.; Delincé, J.

doi:10.1016/0531-5565(89)90017-x

Cited by 39 publications

(23 citation statements)

References 9 publications

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“…In this study, mean and maximum lifespan of males (N60) at 21kPa P O2 were 26days and 39days, respectively (Table4). Although these lifespans were low relative to some other studies (Shen et al, 2009), they are similar to previously reported lifespan data on the Oregon-R strain (Lints et al, 1989). The oxygen level experienced by the larvae probably affected survival (Frazier et al, 2001) and density, which could affect lifespan.…”

Section: Effect Of Adult Ambient Oxygen On Lifespansupporting

confidence: 66%

Lifespan and oxidative stress show a non-linear response to atmospheric oxygen inDrosophila

Rascón

Harrison

2010

Journal of Experimental Biology

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SUMMARYOxygen provides the substrate for most ATP production, but also serves as a source of reactive oxygen species (ROS), which can induce cumulative macromolecular oxidative damage and cause aging. Pure oxygen atmospheres (100kPa) are known to strongly reduce invertebrate lifespan and induce aging-related physiological changes. However, the nature of the relationship between atmospheric oxygen, oxidative stress, and lifespan across a range of oxygen levels is poorly known. Developmental responses are likely to play a strong role, as prior research has shown strong effects of rearing oxygen level on growth, size and respiratory system morphology. In this study, we examined (1) the effect of oxygen on adult longevity and (2) the effect of the oxygen concentration experienced by larvae on adult lifespan by rearing Drosophila melanogaster in three oxygen atmospheres throughout larval development (10, 21 and 40kPa), then measuring the lifespan of adults in five oxygen tensions (2, 10, 21, 40, 100kPa). We also assessed the rate of protein carbonyl production for flies kept at 2, 10, 21, 40 and 100kPa as adults (all larvae reared in normoxia). The rearing of juveniles in varying oxygen treatments affected lifespan in a complex manner, and the effect of different oxygen tensions on adult lifespan was non-linear, with reduced longevity and heightened oxidative stress at extreme high and low atmospheric oxygen levels. Moderate hypoxia (10kPa) extended maximum, but not mean lifespan.

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Section: Effect Of Adult Ambient Oxygen On Lifespansupporting

confidence: 66%

Lifespan and oxidative stress show a non-linear response to atmospheric oxygen inDrosophila

Rascón

Harrison

2010

Journal of Experimental Biology

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“…This last point agrees well with the obser vations of Lints et al [13] reported in a paper entitled 'Unexplained variations in life-span of the Oregon-R strain of Drosophila melanogaster over a four-year period'. During a Clare [5] at high larval density year period, they observed large variations of life-span, which could reach 46% in a matter of a few generations, in the Oregon-R strain kept in classical conditions.…”

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confidence: 82%

Selection for Increased Longevity in <i>Drosophila melanogaste</i><i>r</i>: A New Interpretation

Baret

Lints²

1993

Gerontology

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The study of the genetic determinism of longevity in Drosophila melanogaster has made use of the technique of late-age reproduction. At low larval density, that indirect selection showed no effect. At high larval density, however, increased mean life-span in lines reproduced at late age was observed. When these last data are examined as a function of the number of days after the beginning of the experiment, instead of as a function of generations, the difference in life-span between early and late lines at high larval density disappears. The erratic evolutions of mean longevities in all the experiments here described may be attributed to unexplained variations in life-span previously observed in a 4-year experiment. Considering the time lag between the measurement of a given generation in early and late lines, the experiments of reproduction at late age cannot demonstrate the genetic determinism of longevity.

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“…Comparison with previous studies An Oregon-R population of Drosophila has been reported to show large and presumably non-random variations in their mean life span values when they are continuously assayed over long periods of time (Lints & Hoste, 1979;Lints et al, 1989). The experiments described here took approximately 2 years to complete; they could clearly have been subjected to these same undefined and inexplicable variations.…”

Section: Discussionmentioning

confidence: 95%

Chromosomal localization and regulation of the longevity determinant genes in a selected strain of Drosophila melanogaster

et al. 1993

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A controlled chromosome substitution experiment was performed on a strain (NDC-L) selected for long life to determine if the genes responsible for the extended-longevity phenotype could be localized to any particular chromosome(s). All 27 different possible combinations of the three major chromosomes of Drosophila melanogaster were constructed and longevities were determined on 3875 individual animals of both sexes and analysed. The results are statistically significant and demonstrate that mean longevity is specified primarily by recessive genes on the third chromosome (c3). The extended longevity phenotype (ELP) is only expressed in those lines which are homozygous for the NDC-L type c3. Loci on the first (ci) and second (c2) chromosomes interact, both positively (ci) and negatively (c2), respectively, such that ci represses c2 which in turn represses c3. The ELP is fully expressed in the mutual presence and mutual absence of ci and c2. The significance of these results is discussed in the context of broader categories of molecular genetic mechanisms suggested previously to be involved in the modulation of longevity in Drosophila.

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Unexplained variations in life span of the Oregon-R strain of Drosophila melanogaster over a four-year period

Cited by 39 publications

References 9 publications

Lifespan and oxidative stress show a non-linear response to atmospheric oxygen inDrosophila

Lifespan and oxidative stress show a non-linear response to atmospheric oxygen inDrosophila

Selection for Increased Longevity in <i>Drosophila melanogaste</i><i>r</i>: A New Interpretation

Chromosomal localization and regulation of the longevity determinant genes in a selected strain of Drosophila melanogaster

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