Unexpected distribution of hepatitis C virus genotypes in patients on hemodialysis and kidney transplant recipients

Perez, Renata M.; Ferraz, Maria Lúcia Gomes; Figueiredo, Margarida Garcia de; Contado, Denilson; Koide, Sandra; Ferreira, Adalgisa P.; Neto, Miguel Cendoroglo; Medina-Pestana, J.O.; Silva, Antonio Eduardo B.

doi:10.1002/jmv.10336

Cited by 28 publications

(21 citation statements)

References 38 publications

(44 reference statements)

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“…However, the predominance of genotype 1, subtype 1a was also found in other Brazilian studies carried out among hemodialysis patients 1,8,10,15,16,19,23 . These data suggest that the high frequency of subtype 1a is probably a consequence of HCV nosocomial transmission in hemodialysis units.…”

Section: Resultssupporting

confidence: 71%

“…Studies carried out in São Paulo 15,16,19 , Rio de Janeiro 3 , Belo Horizonte 7 , Recife 1 , Tocantins 23 , Goiás 8 and Campo Grande 10 have shown that genotype 1, subtype 1a, was prevalent in those patients. However, the genotyping methods employed in the majority of those studies (except in Goiás State), which were based on 5' NC region analyses, did not permit the correct identification of the HCV subtypes.…”

Section: Introductionmentioning

confidence: 99%

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Hepatitis C virus genotypes in hemodialysis patients in the Federal District, Brazil

Amorim

Raiol

Trevizoli

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYHepatitis C virus (HCV) genotypes and subtypes were determined in hemodialysis patients in the Federal District, Brazil, by sequencing of the 5' noncoding (NC) and nonstructural 5B (NS5B) regions. From 761 patients, 66 anti-HCV-positive samples were tested for HCV RNA. All 51 HCV RNA-positive samples by PCR of the 5' NC region were genotyped as genotypes 1 (90.2%) and 3 (9.8%). Subtype 1a (82.3%) was the most prevalent, followed by subtypes 3a (9.8%), 1b (5.9%) and 1a/1b (2.0%). Forty-two samples could be amplified and genotyped in the NS5B region: 38 (90.5%) as genotype 1, subtypes 1a, and 8 (9.5%) as genotype 3, subtype 3a. For the 42 samples sequenced in both regions, the genotypes and subtypes determined were concordant in 100% and 95.2% of cases, respectively. Two samples presented discrepant results, with the 5' NC region not distinguishing correctly the subtypes 1a and 1b. These findings indicate that the HCV genotype 1, subtype 1a, is the most prevalent among hemodialysis patients in the Federal District, Brazil.

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Section: Resultssupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Hepatitis C virus genotypes in hemodialysis patients in the Federal District, Brazil

Amorim

Raiol

Trevizoli

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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show abstract

“…The distribution of HCV genotypes and subtypes found in the present study was similar to those previously reported in Brazilian hemodialysis patients (6)(7)(8)(9), who showed a predominance of HCV subtype 1a. These data suggest that subtype 1a is more likely to disseminate in the hemodialysis environment or could be more adapted to the immunosuppression of these patients (7).…”

supporting

confidence: 90%

“…These data suggest that subtype 1a is more likely to disseminate in the hemodialysis environment or could be more adapted to the immunosuppression of these patients (7).…”

mentioning

confidence: 88%

Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis

Espírito-Santo

Carneiro

Reis

et al. 2007

Braz J Med Biol Res

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The present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1095 sera were tested for HCV RNA by RTnested PCR of the 5' noncoding region. The LiPA assay was able to genotype all 131 HCV RNA-positive samples. Genotypes 1 (92.4%) and 3 (7.6%) were found. Subtype 1a (65.7%) was the most prevalent, followed by subtypes 1b (26.7%) and 3a (7.6%). Direct nucleotide sequencing of 340 bp from the NS5B region was performed in 106 samples. The phylogenetic tree showed that 98 sequences (92.4%) were classified as genotype 1, subtypes 1a (72.6%) and 1b (19.8%), and 8 sequences (7.6%) as subtype 3a. The two genotyping methods gave concordant results within HCV genotypes and subtypes in 100 and 96.2% of cases, respectively. Only four samples presented discrepant results, with LiPA not distinguishing subtypes 1a and 1b. Therefore, HCV genotype 1 (subtype 1a) is predominant in hemodialysis patients in Central Brazil. By using sequence analysis of the NS5B region as a reference standard method for HCV genotyping, we found that LiPA was efficient at the genotype level, although some discrepant results were observed at the subtype level (sensitivity of 96.1% for subtype 1a and 95.2% for subtype 1b). Thus, analysis of the NS5B region permitted better discrimination between HCV subtypes, as required in epidemiological investigations.

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“…HCV RNA was detected by qualitative PCR using Amplicor kits (Cobas Amplicor HCV Test, Roche Diagnostic System, USA; detection limit: 50 IU/mL) and HCV genotyping was performed by sequencing the NS5B region of HCV 30 .…”

Section: Methodsmentioning

confidence: 99%

Chronic hepatitis C: hepatic iron content does not correlate with response to antiviral therapy

Pereira

Silva

Uehara

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe complex interaction between hepatitis C virus infection, iron homeostasis and the response to antiviral treatment remains controversial. The aim of this study was to evaluate the influence of hepatic iron concentration (HIC) on the sustained virological response (SVR) to antiviral therapy in patients with chronic hepatitis C. A total of 50 patients who underwent pretreatment liver biopsy with assessment of HIC by graphite furnace atomic absorption spectroscopy and were subsequently submitted to antiviral treatment with interferon/peginterferon and ribavirin were included in the study. Patients with alcoholism, history of multiple blood transfusion, chronic kidney disease, hemolytic anemia and parenteral iron therapy were excluded. The iron related markers and HIC were compared between those who achieved an SVR and non-responders (NR) patients. The mean age was 45.7 years and the proportion of patients' gender was not different between SVR and NR patients. The median serum iron was 138 and 134 µg/dL (p = 0.9), the median serum ferritin was 152.5 and 179.5 ng/mL (p = 0.87) and the median HIC was 9.9 and 8.2 µmol/g dry tissue (p = 0.51), for SVR and NR patients, respectively. Thus, hepatic iron concentration, determined by a reliable quantitative method, was not a negative predictive factor of SVR in patients with chronic hepatitis C presenting mild to moderate hepatic iron accumulation.

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Unexpected distribution of hepatitis C virus genotypes in patients on hemodialysis and kidney transplant recipients

Cited by 28 publications

References 38 publications

Hepatitis C virus genotypes in hemodialysis patients in the Federal District, Brazil

Hepatitis C virus genotypes in hemodialysis patients in the Federal District, Brazil

Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis

Chronic hepatitis C: hepatic iron content does not correlate with response to antiviral therapy

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