Unequivocal Cardiac Phase Sorting From Alternating Ramp-And Pulse-Illuminated Microscopy Image Sequences

Mariani, Olivia; Marelli, François; Jaques, Christian; Ernst, Alexander; Liebling, Michael

doi:10.1109/isbi48211.2021.9433858

Cited by 2 publications

(10 citation statements)

References 10 publications

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“…Previous works used simplified simulation models of the heart for performance characterization, consisting of a circle (or cylinder) textured with a sinusoidal pattern, with a radius varying over time to represent beating (22,(25)(26)(27) . These models lack properties such as irregular texture, asymmetric contraction or small variations in the periodicity that we find in experimental cardiac images.…”

Section: Methods: Fluorescence Cardiac Imaging Simulationmentioning

confidence: 99%

“…In previous work (27) , we have introduced a technique that uses active illumination to acquire pairs of images in quick succession by alternating between illumination patterns. The first image in a pair encodes movement information for unequivocally sorting the sequence in order of increasing phase, while the second uses a short light flash that produces a sharp image used for analysis and display of the reconstructed sequence.…”

Section: Implementing Paaq With Active Illuminationmentioning

confidence: 99%

“…Therefore, we shape 𝑟 (𝑡) to capture most information at the end of the exposure period, minimizing the delay between images in a pair. Instead of the continuous ramp used in the original method (27) we use a series of pulses of increasing amplitude for 𝑟 (𝑡), as this pattern is easier to generate while retaining the same motion-encoding properties as the ramp.…”

Section: Implementing Paaq With Active Illuminationmentioning

confidence: 99%

“…The frequency information, which was missing in previous sorting-based virtual high frame rate methods (25,27,46) , allows to study the heart rate of the sample. It also allows converting phases into time units, which is better suited for analysing the dynamics of the heartbeat.…”

Section: Reconstructing Signal Sequencesmentioning

confidence: 99%

“…In a previous work, we introduced a method to disambiguate images that appeared similar despite being captured in different phases of the heartbeat. We paired sharp images with blurred images that encode the motion at the time of acquisition (27) . In this paper, we extend this technique into a general approach centred around Paired Alternating AcQuisitions (PAAQ), which result in image sequences whose frames alternate between a common reference modality and other channels (e.g., fluorescence channels).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

PAAQ: Paired Alternating AcQuisitions for Virtual High Frame Rate Multichannel Cardiac Fluorescence Microscopy

Marelli¹,

Ernst²,

Mercader³

et al. 2023

Preprint

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In vivo fluorescence microscopy is a powerful tool to image the beating heart in its early development stages. A high acquisition frame rate is necessary to study its fast contractions, but the limited fluorescence intensity requires sensitive cameras that are often too slow. Moreover, the problem is even more complex when imaging distinct tissues in the same sample using different fluorophores. We present Paired Alternating AcQuisitions (PAAQ), a method to image cyclic processes in multiple channels, which requires only a single (possibly slow) camera.We generate variable temporal illumination patterns in each frame, alternating between channel-specific illuminations (fluorescence) in odd frames and a motion-encoding brightfield pattern as a common reference in even frames. Starting from the image pairs, we find the position of each reference frame in the cardiac cycle through a combination of image-based sorting and regularized curve fitting. Thanks to these estimated reference positions, we assemble multichannel videos whose frame rate is virtually increased.We characterize our method on synthetic and experimental images collected in zebrafish embryos, showing quantitative and visual improvements in the reconstructed videos over existing non-gated sorting-based alternatives. Using a 15 Hz camera, we showcase a reconstructed video containing 2 fluorescence channels at 100 fps.

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Section: Methods: Fluorescence Cardiac Imaging Simulationmentioning

confidence: 99%

Section: Implementing Paaq With Active Illuminationmentioning

confidence: 99%

Section: Implementing Paaq With Active Illuminationmentioning

confidence: 99%

Section: Reconstructing Signal Sequencesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

PAAQ: Paired Alternating AcQuisitions for Virtual High Frame Rate Multichannel Cardiac Fluorescence Microscopy

Marelli¹,

Ernst²,

Mercader³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

PAAQ: Paired Alternating AcQuisitions for virtual high frame rate multichannel cardiac fluorescence microscopy

Marelli,

Ernst,

Mercader

et al. 2023

Biol. Imaging

Self Cite

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In vivo fluorescence microscopy is a powerful tool to image the beating heart in its early development stages. A high acquisition frame rate is necessary to study its fast contractions, but the limited fluorescence intensity requires sensitive cameras that are often too slow. Moreover, the problem is even more complex when imaging distinct tissues in the same sample using different fluorophores. We present Paired Alternating AcQuisitions, a method to image cyclic processes in multiple channels, which requires only a single (possibly slow) camera. We generate variable temporal illumination patterns in each frame, alternating between channel-specific illuminations (fluorescence) in odd frames and a motion-encoding brightfield pattern as a common reference in even frames. Starting from the image pairs, we find the position of each reference frame in the cardiac cycle through a combination of image-based sorting and regularized curve fitting. Thanks to these estimated reference positions, we assemble multichannel videos whose frame rate is virtually increased. We characterize our method on synthetic and experimental images collected in zebrafish embryos, showing quantitative and visual improvements in the reconstructed videos over existing nongated sorting-based alternatives. Using a 15 Hz camera, we showcase a reconstructed video containing two fluorescence channels at 100 fps.

show abstract

Unequivocal Cardiac Phase Sorting From Alternating Ramp-And Pulse-Illuminated Microscopy Image Sequences

Cited by 2 publications

References 10 publications

PAAQ: Paired Alternating AcQuisitions for Virtual High Frame Rate Multichannel Cardiac Fluorescence Microscopy

PAAQ: Paired Alternating AcQuisitions for Virtual High Frame Rate Multichannel Cardiac Fluorescence Microscopy

PAAQ: Paired Alternating AcQuisitions for virtual high frame rate multichannel cardiac fluorescence microscopy

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