Undesired fertility restoration in msm1 barley associates with two mTERF genes

Bernhard, Timm; Koch, Michael; Snowdon, Rod J.; Friedt, Wolfgang; Wittkop, Benjamin

doi:10.1007/s00122-019-03281-9

Cited by 19 publications

(20 citation statements)

References 63 publications

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“…Known Rf genes belong to a distinct clade within the family of pentatricopeptide repeat (PPR) RNA-binding factors, whose encoded proteins are referred to as Rf -like (RFL) 49,50 . Members of the mitochondrial transcription TERmination Factor (mTERF) family are likely also involved in fertility restoration in cereals 51 . The repertoire of restorer genes is predicted to expand in outcrossing species 35,52 .…”

Section: Resultsmentioning

confidence: 99%

Chromosome-scale genome assembly provides insights into rye biology, evolution, and agronomic potential

Rabanus‐Wallace

Hackauf

Mascher

et al. 2019

Preprint

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We present a chromosome-scale annotated assembly of the rye (Secale cereale L. inbred line ‘Lo7’) genome, which we use to explore Triticeae genomic evolution, and rye’s superior disease and stress tolerance. The rye genome shares chromosome-level organization with other Triticeae cereals, but exhibits unique retrotransposon dynamics and structural features. Crop improvement in rye, as well as in wheat and triticale, will profit from investigations of rye gene families implicated in pathogen resistance, low temperature tolerance, and fertility control systems for hybrid breeding. We show that rye introgressions in wheat breeding panels can be characterised in high-throughput to predict the yield effects and trade-offs of rye chromatin.

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Section: Resultsmentioning

confidence: 99%

Chromosome-scale genome assembly provides insights into rye biology, evolution, and agronomic potential

Rabanus‐Wallace

Hackauf

Mascher

et al. 2019

Preprint

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“…Pan et al (2019) identified the candidate gene Zea mays small kernel 3 (Zmsmk3), which contained two mTERF motifs and was required for the intron splicing of mitochondrial nad4 and nad1 genes and kernel development. Genome-wide association studies in a multiparental mapping population in hybrid barley detected two mTERF proteins linked to the restorer locus Rfm3 on the short arm of chromosome 6H (Bernhard et al, 2019). A group of candidate genes belonging to the PPR family was also identified in the physical region associated with QRf.lfl-6AS.1.…”

Section: Discussionmentioning

confidence: 99%

Identification of Rf9, a Gene Contributing to the Genetic Complexity of Fertility Restoration in Hybrid Wheat

et al. 2020

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Wheat (Triticum aestivum L.) is a self-pollinating crop whose hybrids offer the potential to provide a major boost in yield. Male sterility induced by the cytoplasm of Triticum timopheevii is a powerful method for hybrid seed production. Hybrids produced by this method are often partially sterile, and full fertility restoration is crucial for wheat production using hybrid cultivars. To identify the genetic loci controlling fertility restoration in wheat, we produced two cytoplasmic male-sterile (CMS) backcross (BC1) mapping populations. The restorer lines Gerek 79 and 71R1203 were used to pollinate the male-sterile winter wheat line CMS-Sperber. Seed set and numbers of sterile spikelets per spike were evaluated in 340 and 206 individuals of the populations derived from Gerek 79 and 71R1203, respectively. Genetic maps were constructed using 930 and 994 single nucleotide polymorphism (SNP) markers, spanning 2,160 and 2,328 cM over 21 linkage groups in the two populations, respectively. Twelve quantitative trait loci (QTL) controlled fertility restoration in both BC1 populations, including a novel restorer-of-fertility (Rf) locus flanked by the SNP markers IWB72413 and IWB1550 on chromosome 6AS. The locus was mapped as a qualitative trait in the BC1 Gerek 79 population and was designated Rf9. One hundred-nineteen putative candidate genes were predicted within the QTL region on chromosome 6AS. Among them were genes encoding mitochondrial transcription termination factor and pentatricopeptide repeat-containing proteins that are known to be associated with fertility restoration. This finding is a promising step to better understand the functions of genes for improving fertility restoration in hybrid wheat.

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“…Barley Rfm3 is genetically associated with two linked genes encoding mitochondrial transcription termination factor family (mTERF) proteins [39]. Interestingly, rye Rfp1 shows tight linkage with an mTERF protein gene [40].…”

Section: What Do Rf S Encode Other Than Ppr Proteins?mentioning

confidence: 99%

“…The organizational diversity of non-PPR Rf s in genetic resources is less investigated. Several haplotypes based on single nucleotide polymorphisms and small insertion/deletions were identified in two linked mTERF protein genes at the barley Rfm3 locus, and one haplotype is exclusively found from a non-restoring genotype [39].…”

Section: Evolution Of Non-ppr Rfsmentioning

confidence: 99%

“…Some non-PPR Rf s are also associated with post-transcriptional mechanisms such as in rice Rf2 [38]. It is possible that barley Rfm3 is associated with this mechanism by analogy with mTERF protein function (this might also be the case for rye Rfp1) [39,40,54], but identifying the mitochondrial gene responsible for CMS and studying the effect of Rf at the molecular level is necessary.…”

Section: Group 1: Association With a Post-transcriptional Mechanism Fmentioning

confidence: 99%

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What Does the Molecular Genetics of Different Types of Restorer-of-Fertility Genes Imply?

Kubo

Arakawa

Honma

et al. 2020

Plants

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Cytoplasmic male sterility (CMS) is a widely used trait for hybrid seed production. Although male sterility is caused by S cytoplasm (male-sterility inducing mitochondria), the action of S cytoplasm is suppressed by restorer-of-fertility (Rf), a nuclear gene. Hence, the genetics of Rf has attained particular interest among plant breeders. The genetic model posits Rf diversity in which an Rf specifically suppresses the cognate S cytoplasm. Molecular analysis of Rf loci in plants has identified various genes; however, pentatricopeptide repeat (PPR) protein (a specific type of RNA-binding protein) is so prominent as the Rf-gene product that Rfs have been categorized into two classes, PPR and non-PPR. In contrast, several shared features between PPR- and some non-PPR Rfs are apparent, suggesting the possibility of another grouping. Our present focus is to group Rfs by molecular genetic classes other than the presence of PPRs. We propose three categories that define partially overlapping groups of Rfs: association with post-transcriptional regulation of mitochondrial gene expression, resistance gene-like copy number variation at the locus, and lack of a direct link to S-orf (a mitochondrial ORF associated with CMS). These groups appear to reflect their own evolutionary background and their mechanism of conferring S cytoplasm specificity.

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Undesired fertility restoration in msm1 barley associates with two mTERF genes

Cited by 19 publications

References 63 publications

Chromosome-scale genome assembly provides insights into rye biology, evolution, and agronomic potential

Chromosome-scale genome assembly provides insights into rye biology, evolution, and agronomic potential

Identification of Rf9, a Gene Contributing to the Genetic Complexity of Fertility Restoration in Hybrid Wheat

What Does the Molecular Genetics of Different Types of Restorer-of-Fertility Genes Imply?

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