Undesirable consequences of the metallic nanoparticles action on the properties and functioning of Escherichia coli, Bacillus cereus and Staphylococcus epidermidis membranes

Metryka, Oliwia; Wasilkowski, Daniel; Adamczyk-Habrajska, Małgorzata; Mrozik, Agnieszka

doi:10.1016/j.jhazmat.2023.130728

Cited by 10 publications

(10 citation statements)

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“…The potential formulation of copper complexes and Cu-GO composites as novel antimicrobial materials holds promise, considering their demonstrated efficacy and the added advantage of excellent optical properties. Nanoparticles with antimicrobial activities have been extensively explored in various studies, with the reported accumulation in the Staphylococcus membrane supporting the observed antimicrobial effects [49]. This aligns with the growing body of research on nanomaterials combating microbial threats, emphasizing the importance of novel approaches in developing effective antimicrobial agents.…”

Section: Discussionmentioning

confidence: 63%

Fine-Tuned Graphene Oxide Nanocomposite: Harnessing Copper(II)–Imidazole Complex for Enhanced Biological Responses and Balanced Photocatalytic Functionality

Sundaram,

Kumaravelu,

Tseng

et al. 2024

Materials

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In this study, the synthesis of biologically active copper(II) complex [Cu(im)2]Cl2 was achieved using a reported method. Subsequently, this copper(II) complex was strategically grafted onto graphene oxide, resulting in the formation of a nanocomposite denoted as copper(II)-complex-grafted graphene oxide (Cu-GO). The comprehensive characterization of Cu-GO was conducted through various techniques, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), UV–visible spectroscopy, emission spectra analysis, X-ray photoelectron spectroscopy (XPS), and Copper K-edge X-ray Absorption Near Edge Structure (XANES) spectroscopy. The antibacterial efficacy of Cu-GO compounds was assessed using disk diffusion and microbroth dilution methods. Notably, the copper complex exhibited the highest effectiveness, showcasing a Minimal Inhibitory Concentration (MIC) value of 500 µL against Klebsiella bacteria. The antibacterial activities of all compounds were systematically screened, revealing the superior performance of the copper complex compared to standalone copper compounds. Expanding the scope of the investigation, we explored the antioxidant and anti-obesity activities of the copper complexes against Klebsiella organisms. The results underscore promising directions for the further exploration of the diverse health-related applications of these compounds. Moreover, the photocatalytic performance of the Cu-GO nanocomposite was evaluated under sunlight irradiation. Notably, the antioxidant and anti-obesity activities of Cu-GO, assessed in terms of percentage inhibition at a concentration of 200 mg/mL, exhibited values of 41% and 45%, respectively. Additionally, the Cu-GO composite exhibited exceptional efficacy, achieving a degradation efficiency of 74% for RhB under sunlight irradiation, surpassing both graphite and GO. These findings not only demonstrate enhanced biological activity, but also highlight a notable level of moderate photocatalytic performance. Such dual functionality underscores the potential versatility of Cu-GO nanocomposites across various applications, blending heightened biological efficacy with controlled photocatalysis. Our study offers valuable insights into the multifunctional attributes of copper(II)-complex-grafted graphene oxide nanocomposites, thereby paving the way for their broader utilization in diverse fields.

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Section: Discussionmentioning

confidence: 63%

Fine-Tuned Graphene Oxide Nanocomposite: Harnessing Copper(II)–Imidazole Complex for Enhanced Biological Responses and Balanced Photocatalytic Functionality

Sundaram,

Kumaravelu,

Tseng

et al. 2024

Materials

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“…US1019F, US Research, 20 nm) on the structure and properties of Gram-positive Bacillus cereus (ATCC ® 11778 TM ) and Staphylococcus epidermidis (ATCC ® 12228 TM ) outer layers. The performed analyses concerned the assessment of cell membrane permeability, determination of cytoplasmic leakage, cellular ATP levels and ATPase activity, changes in the fatty acid profiling and the distribution and specific interactions of NPs with the surface of bacterial cells [4].The results confirmed the differentiated effects of inorganic NPs on the metabolic processes and structure of tested bacteria, depending on their concentration and type of NPs. Undeniably, NPs caused significant changes in the permeability of the bacterial cell membrane, which were correlated with alternations in the leakage of intracellular contents together with the cellular ATP concentrations.…”

mentioning

confidence: 59%

“…Furthermore, it was established that B. cereus was more resistant to the NPs toxic activity than S. epidermidis. The multi-directional analysis indisputably contributed to a thorough comprehension of NPs biological activity toward microorganisms and provided insight into their distinguished interactions with bacterial outer layers [4]. The collected dataset fills a gap in the literature regarding NPs toxicity and complex molecular action on microbial cells and constitutes a valuable contribution to future research.…”

mentioning

confidence: 98%

“…In particular, little is known about the interaction of NPs with the bacterial cell wall and cytoplasmic membrane. These mechanisms require further and in-depth elucidation because direct and indirect interactions of NPs with the bacterial outer layers can lead to permanent damage to protective barriers, destabilisation of membrane potential, structural changes, increased membrane permeability and lipid peroxidation, disruption of transport activity, uncoupling of oxidative phosphorylation, leakage of intracellular contents and disturbances in the respiratory metabolism [2][3][4].This research aimed to explore and understand the action of metal-based NPs including Ag-NPs (cat. no.…”

mentioning

confidence: 99%

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confidence: 99%

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Targeted Mechanisms of Action of Metal-Based Nanoparticles on Gram-Positive Bacteria Cell Envelopes

Metryka,

Wasilkowski,

Mrozik

2024

Proceedings of the 9th World Congress on Recent Advances in Nanotechnology

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Over the last few decades, nanotechnology has become one of the leading and most promising scientific discoveries, offering extraordinary progress in the research, production, and practical application of nanomaterials. The global nanotechnology is undeniably dominated by metal-based nanoparticles (NPs). The exponential growth in the production and applications of NPs is related to their distinctive and valuable properties, distinguishing them from their larger-scale counterparts. A unique feature of inorganic NPs is their antimicrobial activity, consisting of a multi-faceted, pleiotropic, and non-specific mode of action on various biomolecules and metabolic processes. Due to the rise in the production of NPs, large amounts of NPs are released uncontrollably and accumulated in the natural environment, posing an inevitable threat to microorganisms and other living organisms. Although the number of studies on the synthesis and use of NPs is growing dynamically, reports on the impact of NPs on the functionality of microorganisms (especially non-target microorganisms) are still insufficient [1,2]. In particular, little is known about the interaction of NPs with the bacterial cell wall and cytoplasmic membrane. These mechanisms require further and in-depth elucidation because direct and indirect interactions of NPs with the bacterial outer layers can lead to permanent damage to protective barriers, destabilisation of membrane potential, structural changes, increased membrane permeability and lipid peroxidation, disruption of transport activity, uncoupling of oxidative phosphorylation, leakage of intracellular contents and disturbances in the respiratory metabolism [2][3][4].This research aimed to explore and understand the action of metal-based NPs including Ag-NPs (cat. no. 576832, Sigma-Aldrich, <100 nm), Cu-NPs (cat. no. 774081, Sigma-Aldrich, 25 nm), ZnO-NPs (cat. no. 677450, Sigma-Aldrich, <50 nm) and TiO 2 -NPs (cat. no. US1019F, US Research, 20 nm) on the structure and properties of Gram-positive Bacillus cereus (ATCC ® 11778 TM ) and Staphylococcus epidermidis (ATCC ® 12228 TM ) outer layers. The performed analyses concerned the assessment of cell membrane permeability, determination of cytoplasmic leakage, cellular ATP levels and ATPase activity, changes in the fatty acid profiling and the distribution and specific interactions of NPs with the surface of bacterial cells [4].The results confirmed the differentiated effects of inorganic NPs on the metabolic processes and structure of tested bacteria, depending on their concentration and type of NPs. Undeniably, NPs caused significant changes in the permeability of the bacterial cell membrane, which were correlated with alternations in the leakage of intracellular contents together with the cellular ATP concentrations. Consequently, NPs induced considerable modifications in the composition and percentages of the analysed fatty acids, especially within the cyclopropane fatty acid abundances. Furthermore, it was established that B. cereus was more resistant to the...

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Metallic nanoparticle actions on the outer layer structure and properties of Bacillus cereus and Staphylococcus epidermidis

Metryka,

Wasilkowski,

Dulski

et al. 2024

Chemosphere

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Undesirable consequences of the metallic nanoparticles action on the properties and functioning of Escherichia coli, Bacillus cereus and Staphylococcus epidermidis membranes

Cited by 10 publications

References 54 publications

Fine-Tuned Graphene Oxide Nanocomposite: Harnessing Copper(II)–Imidazole Complex for Enhanced Biological Responses and Balanced Photocatalytic Functionality

Fine-Tuned Graphene Oxide Nanocomposite: Harnessing Copper(II)–Imidazole Complex for Enhanced Biological Responses and Balanced Photocatalytic Functionality

Targeted Mechanisms of Action of Metal-Based Nanoparticles on Gram-Positive Bacteria Cell Envelopes

Metallic nanoparticle actions on the outer layer structure and properties of Bacillus cereus and Staphylococcus epidermidis

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