Understanding trophic interactions of <i>Orius</i>
 spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods

Gomez-Polo, Priscila; Alomar, Òscar; Castañé, Cristina; Aznar-Fernández, T.; Lundgren, Jonathan G.; Piñol, Josep; Agustí, Núria

doi:10.1002/ps.3989

Cited by 35 publications

(27 citation statements)

References 52 publications

(107 reference statements)

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“…This technique builds upon conventional PCR by including a fluorescent dye that binds to double-stranded DNA, and thus the quantity of DNA produced in each PCR cycle is monitored using a spectrophotometer during the PCR process27. It requires a special thermocycler and specific reagents used for fluorescence, but does not require equipment associated with gel analysis used in conventional PCR, that could detect a higher frequency of insect food consumption than conventional PCR when both tests are run at optimal conditions2829. It has been used in pathology and vector insect research303132, to investigate the relationship between humans and mosquitoes33, as well as insect classification, insect nucleotide polymorphism, insect toxicology34, insect physiology35, and mostly focusing on insect molecular ecology28293637383940 et al .…”

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confidence: 99%

Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species

Huang

McNeill

et al. 2016

Sci Rep

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Studies on grasshopper diets have historically employed a range of methodologies, each with certain advantages and disadvantages. For example, some methodologies are qualitative instead of quantitative. Others require long experimental periods or examine population-level effects, only. In this study, we used real-time PCR to examine diets of individual grasshoppers. The method has the advantage of being both fast and quantitative. Using two grasshopper species, Oedaleus asiaticus and Dasyhippus barbipes, we designed ITS primer sequences for their three main host plants, Stipa krylovii, Leymus chinensis and Cleistogenes squarrosa and used real-time PCR method to test diet structure both qualitatively and quantitatively. The lowest detection efficiency of the three grass species was ~80% with a strong correlation between actual and PCR-measured food intake. We found that Oedaleus asiaticus maintained an unchanged diet structure across grasslands with different grass communities. By comparison, Dasyhippus barbipes changed its diet structure. These results revealed why O. asiaticus distribution is mainly confined to Stipa-dominated grassland, and D. barbipes is more widely distributed across Inner Mongolia. Overall, real-time PCR was shown to be a useful tool for investigating grasshopper diets, which in turn offers some insight into grasshopper distributions and improved pest management.

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confidence: 99%

Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species

Huang

McNeill

et al. 2016

Sci Rep

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“…Feeding trials to determine the detection period of H. zea DNA in the gutcontents of O. insidiosus revealed a half-life of only 2.32 h. This is a shorter detection window than those reported for Orius spp. screened against primer pairs specific to species of aphids, thrips, whitefly, and ladybeetle, which ranged from 2.7 to 21.8 h (Gomez-Polo et al, 2016;Harwood et al, 2007;Simmons et al, 2015). Predatory hemipterans are one of the taxa found to have a wide range of detectability half-lives (Greenstone et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The use of DNA-based techniques, such as polymerase chain reaction (PCR) with prey-specific primers can provide a better technique that allows for the determination of trophic relationships without interference in ecological interactions occurring in the field, and has been successfully used with Orius spp. (Gomez-Polo et al, 2016;Harwood et al, 2007;Simmons et al, 2015). Therefore, in this study, PCR molecular gut-content analysis was used to determine the frequency of predation of a critical pest, corn earworm H. zea, by a predator, the insidious flower bug O. insidiosus, in sweet corn agroecosystems in Minnesota.…”

Section: Introductionmentioning

confidence: 99%

Molecular gut-content analysis reveals high frequency of Helicoverpa zea (Lepidoptera: Noctuidae) consumption by Orius insidiosus (Hemiptera: Anthocoridae) in sweet corn

et al. 2018

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Management of corn earworm Helicoverpa zea in sweet corn grown for processing can be challenging due to the lack of effective transgenic and chemical control options. However, biological control by generalist predators can provide a significant impact on pests in this cropping system. One of the most ubiquitous predators of H. zea and other lepidopterans is the insidious flower bug, Orius insidiosus. This small hemipteran has been observed as an important mortality agent of H. zea in several cropping systems, but the strength of the trophic connection between these species has not been documented in sweet corn. Molecular gut-content analysis was conducted to test field-collected O. insidiosus for the presence of H. zea DNA using species-specific PCR primers developed and optimized for this project. Controlled feeding trials determined that the detectability half-life of this technique was 2.32 h. At peak predation in late August, 32% of O. insidiosus tested digitalcommons.unl.edu P e t e r s o n e t a l. i n B i o l o g i c a l C o n t r o l 1 2 1 (2 0 1 8) 2 positive for H. zea DNA. The date of peak predation also corresponded with peak silking of sweet corn plants, which is the most attractive crop growth stage to both H. zea and O. insidiosus. These results indicate that within a short window prior to collection from the field, on the peak date of predation, approximately one third of O. insidiosus in sweet corn had consumed one to two H. zea eggs and/or first instar larvae. The demonstration of this high frequency of predation allows for the assertion that O. insidiosus is a critical mortality agent of H. zea in sweet corn, and conservation biological control practices should be explored to protect and promote this key predator.

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“…A particular need, however, is to integrate the results from NGS with ecological methods ( Furlong, 2015 ). An example of such integration is Spanish work on lettuce pests in which NGS was used to study the diet to two major predators ( Gomez-Polo et al, 2015 ). The value of the NGS approach was that it enabled the detection of intra-guild predation, a phenomenon not evident in concurrent PCR assays.…”

Section: Oppourtunities For Use Of Molecular Technologiesmentioning

confidence: 99%

Conservation Biological Control of Pests in the Molecular Era: New Opportunities to Address Old Constraints

Gurr

You

2016

Front. Plant Sci.

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Biological control has long been considered a potential alternative to pesticidal strategies for pest management but its impact and level of use globally remain modest and inconsistent. A rapidly expanding range of molecular – particularly DNA-related – techniques is currently revolutionizing many life sciences. This review identifies a series of constraints on the development and uptake of conservation biological control and considers the contemporary and likely future influence of molecular methods on these constraints. Molecular approaches are now often used to complement morphological taxonomic methods for the identification and study of biological control agents including microbes. A succession of molecular techniques has been applied to ‘who eats whom’ questions in food-web ecology. Polymerase chain reaction (PCR) approaches have largely superseded immunological approaches such as enzyme-linked immunosorbent assay (ELISA) and now – in turn – are being overtaken by next generation sequencing (NGS)-based approaches that offer unparalleled power at a rapidly diminishing cost. There is scope also to use molecular techniques to manipulate biological control agents, which will be accelerated with the advent of gene editing tools, the CRISPR/Cas9 system in particular. Gene editing tools also offer unparalleled power to both elucidate and manipulate plant defense mechanisms including those that involve natural enemy attraction to attacked plants. Rapid advances in technology will allow the development of still more novel pest management options for which uptake is likely to be limited chiefly by regulatory hurdles.

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Understanding trophic interactions of Orius spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods

Cited by 35 publications

References 52 publications

Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species

Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species

Molecular gut-content analysis reveals high frequency of Helicoverpa zea (Lepidoptera: Noctuidae) consumption by Orius insidiosus (Hemiptera: Anthocoridae) in sweet corn

Conservation Biological Control of Pests in the Molecular Era: New Opportunities to Address Old Constraints

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