Understanding the switchable solvent extraction of phospholipids from dairy byproducts

Rathnakumar, Kaavya; Ortega‐Anaya, Joana; Jiménez-Flores, Rafael; Martínez‐Monteagudo, Sergio I.

doi:10.1016/j.fbp.2021.01.003

Cited by 11 publications

(15 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This MFGM and protein-rich by-product is underutilized due to the potential development of a stale, oxidized off-flavor, and brown discoloration by lipid oxidation and Maillard browning reactions (Carunchia Whetstine et al, 2005). Nevertheless, the abundance of PLs and bioactive proteins associated with MFGM still brings a strong interest in further utilization of beta stream (Huang et al, 2020;Rathnakumar et al, 2021a;Rathnakumar et al, 2021b). After calcium acetate treatment, the precipitated MFGM had a lipid content of 27.4% (w/w) and salt and protein content of 31.7% and 30.6% (w/w), respectively.…”

Section: Resultsmentioning

confidence: 99%

Utilization of dairy beta stream to produce phospholipids products by salt precipitation and solvent fractionation

Wan

Tao

Clark

et al. 2022

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Dairy phospholipids (PLs) have unique functional and nutraceutical properties over PLs obtained from other sources that typically lack phosphatidylserine (PS) and sphingomyelin (SM). In this study, an underutilized dairy by‐product, beta stream, was used for the production of enriched dairy PLs products and membrane proteins by solvent extractions. After calcium salt precipitation of the membrane components, various green (i.e., safer or bioderived) solvent systems were evaluated for their capabilities for PLs extraction from the PLs‐protein‐salt precipitate in comparison to the standard Folch extraction (chloroform: methanol, 2:1, v/v). Among the five solvent systems (Folch alternatives) evaluated, the combination of hexane and isopropanol (H:IP, 3:2, v/v) was identified as the best for total lipid and PLs extraction, resulting in a recovery of 74.4% and 65.7% (w/w), respectively. EDTA chelating of the calcium ions was applied to produce a salt‐free membrane protein product. A treatment with 0.2% EDTA was able to significantly reduce the calcium content to 0.20 mg/g protein compared to 58.81 mg/g in the initial calcium‐precipitated MFGM. EDTA chelating of cations also facilitated the extraction of lipids during H:IP (3:2) treatment, which increased the total recovery of total lipid and PLs to 90.7% and 82.5% (w/w), respectively. Overall, this study demonstrates a great potential for the utilization of beta stream and other low‐solids content aqueous dairy by‐products for PLs recovery and further fractionation. Value‐added dairy products (PLs and membrane proteins) can be obtained using green solvents identified in this study, and the process is industrially scalable.

show abstract

Section: Resultsmentioning

confidence: 99%

Utilization of dairy beta stream to produce phospholipids products by salt precipitation and solvent fractionation

Wan

Tao

Clark

et al. 2022

J Americ Oil Chem Soc

View full text Add to dashboard Cite

show abstract

“…The MPLs profile was determined with a UHPLC system (Dionex Ultimate 3000, Thermo Scientific) coupled to a charged aerosol detector (CAD, Dionex Corona Veo RS, Thermo Scientific). The methodology reported by Rathnakumar et al (2021aRathnakumar et al ( , 2021b was followed with modifications. Firstly, the MPLs fraction was dissolved in 1 mL of chloroform: methanol solution (9:1, v/v).…”

Section: Determination Of Individual Phospholipidsmentioning

confidence: 99%

“…Interestingly, some extraction conditions (e.g., a ratio of 1/10, 40 C, and 10 h) resulted in extraction yield of lipids higher than 100% (discontinues line in Figure 1). Interestingly, an incomplete conversion of CyNMe2 into its respective salt has been cited as the responsible factor for overestimated values during the extraction of lipids (Rathnakumar et al, 2021b;Rathnakumar, Osorio-Arias, et al, 2021). An investigation of lipid extraction from microalgae showed up to 24% of residual CyNMe2, possibly due to an incomplete conversion (Boyd et al, 2012) In the presence of CO 2 and water, CyNMe2 switches its hydrophilicity due to an acid-base reaction that converts the amine into bicarbonate salt S C H E M E 1 Conversion of N,N dimethylcyclohexylamine (CyNMe2) into bicarbonate salt in the presence of CO 2 and water.…”

Section: Extraction Of Total Lipidsmentioning

confidence: 99%

“…Relatively low extraction yields of MPLs ($2.5%-14.4%) were obtained after 3 h of extraction and a sample-to-solvent ratio of 1/3 independent of the temperature. Extracting MPLs from βS with CyNMe2 is a solid-liquid transfer process, where simultaneous and countercurrent flows of MPLs and CyNMe2 are accompanied by significant shrinkage and deformation of the protein matrix (Rathnakumar et al, 2021b). Mass transfer within solid-liquid interphase (MPLs leaving and CyNMe2 entering) and the shrinkage of the matrix occur simultaneously, and it is thought to be more predominant at the beginning of the extraction and low values of sample-to-solvent ratio.…”

Section: Extraction Of Milk Phospholipidsmentioning

confidence: 99%

See 1 more Smart Citation

Extraction of phospholipids from beta‐serum using switchable solvents and solid‐phase extraction

Rathnakumar

Ortega‐Anaya

Jiménez-Flores

et al. 2023

J Americ Oil Chem Soc

Self Cite

View full text Add to dashboard Cite

We report the extraction of milk phospholipids (MPLs) from beta‐serum with a tertiary amine (N‐N dimethylcyclohexylamine, CyNMe2). The extraction time (3, 10, and 18 h), temperature (25, 40, and 60°C), and sample‐to‐solvent ratio (1/3, 1/10, and 1/18 v/v) were evaluated according to a factorial design. The extracted lipids were fractionated with solid‐phase extraction and quantified by HPLC‐CAD. Extraction conducted at 60°C yielded values of 14.86 ± 2.95%, 69.79 ± 3.19%, 79.39 ± 5.55% after 3, 10, and 18 h and a ratio of 1/18 (w/w). Microstructure images showed significant damage to the solid matrix that released the inner MPLs into the aqueous medium. The highest extraction yields of MPLs (79.39 ± 5.55%) were obtained after 18 h of extraction at 60°C and a sample‐to‐solvent ratio of 18 (w/w). The recovered fraction was made of phosphatidylinositol (~41%), phosphatidylserine (~6%), phosphatidylethanolamine (~34%), phosphatidylcholine (~9%), and sphingomyelin (~8%). This work outlines different extraction conditions for recovering MPLs from beta‐serum, a dairy byproduct.

show abstract

“…According to this investigation, the most studied switchable hydrophilicity solvents are mainly tertiary amine organic compounds, 61,62 including N , N ‐dimethylcyclohexylamine, N , N ‐dimethylbutylamine, and triethylamine 63 . On the one hand, the switchable hydrophilicity solvents have been used by many scholars in areas of oil or asphaltene extraction, 64 enhanced extraction, and recovery of materials, 65 heavy oil separation and utilization, 66 carbon dioxide treatment, 67 etc. On the other hand, some researchers have used it to prepare convertible gels 68,69 .…”

Section: Introductionmentioning

confidence: 99%

Preparation of ethyl cellulose–glycerol tribenzoate microcapsules in CO₂/N₂‐switchable hydrophilicity solvent and solvent recycling

Zheng

Liu

et al. 2022

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this article, a novel method of microcapsule preparation was provided, that is, ethyl cellulose-glycerol tribenzoate microcapsule (EGM) was prepared using CO 2 /N 2 switchable hydrophilicity solvent(N, N-dimethylcyclohexylamine) combined with an emulsion method. Microcapsules were prepared and solvents were recovered by changing the properties of the solvent. The optimum preparation conditions of EGM were the materials ratio of 20%, the oil-water ratio of 4.5:5.5, emulsifier content of 2%, the Span80-Tween80 ratio of 1:3, and the core-shell ratio of 1. The morphology of EGM is spherical, and the particle size can be adjusted from 0.01 μm to 90 μm by controlling the agitation speed in the synthesis process. Surprisingly, the average yield(Y[%]) reached 95.36%, the effective content(W[%]) reached 41%, and the encapsulation rate(E[%]) reached 90.66% of EGM. The solvent recovery rate(SR[%]) of the first recovered is 96.37%. Significantly, this method not only provides a new idea for the preparation of microcapsules but also expands the application field of switchable hydrophilicity solvents, which makes it has great potential application value in the field of microcapsule preparation in the future.

show abstract

Understanding the switchable solvent extraction of phospholipids from dairy byproducts

Cited by 11 publications

References 36 publications

Utilization of dairy beta stream to produce phospholipids products by salt precipitation and solvent fractionation

Utilization of dairy beta stream to produce phospholipids products by salt precipitation and solvent fractionation

Extraction of phospholipids from beta‐serum using switchable solvents and solid‐phase extraction

Preparation of ethyl cellulose–glycerol tribenzoate microcapsules in CO₂/N₂‐switchable hydrophilicity solvent and solvent recycling

Contact Info

Product

Resources

About

Understanding the switchable solvent extraction of phospholipids from dairy byproducts

Cited by 11 publications

References 36 publications

Utilization of dairy beta stream to produce phospholipids products by salt precipitation and solvent fractionation

Utilization of dairy beta stream to produce phospholipids products by salt precipitation and solvent fractionation

Extraction of phospholipids from beta‐serum using switchable solvents and solid‐phase extraction

Preparation of ethyl cellulose–glycerol tribenzoate microcapsules in CO2/N2‐switchable hydrophilicity solvent and solvent recycling

Contact Info

Product

Resources

About

Preparation of ethyl cellulose–glycerol tribenzoate microcapsules in CO₂/N₂‐switchable hydrophilicity solvent and solvent recycling