Understanding the roles of functional peptides in designing apatite and silica nanomaterials biomimetically using NMR techniques

Iline-Vul, Taly; Adiram-Filiba, Nurit; Matlahov, Irina; Geiger, Yasmin; Abayev, Meital; Keinan‐Adamsky, Keren; Akbey, Ümit; Oschkinat, Hartmut; Goobes, Gil

doi:10.1016/j.cocis.2018.01.012

Cited by 14 publications

(32 citation statements)

References 56 publications

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“…Our In-vitro experiments support such entrapment, with significant shifts in the NMR and Raman peaks, indicating close proximity between the peptide and the mineral. The periodic short-range order, as seen in HRTEM, suggests a very intimate interaction of the forming mineral and the peptide, similarly to a peptide derived from Silaffin3, a silica precipitating protein from diatom (Iline-Vul et al ., 2018). The mineral that we produced in vitro contains many surface Si atoms, forming an open mesoporous structure.…”

Section: Discussionmentioning

confidence: 94%

Siliplant1 (Slp1) protein precipitates silica in sorghum silica cells

Kumar

Adiram-Filiba²,

Blum

et al. 2019

Preprint

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28• Silicon is absorbed by plant roots as silicic acid. The acid moves with the transpiration 29 stream to the shoot, and mineralizes as silica. In grasses, leaf epidermal cells called 30 silica cells deposit silica in most of their volume by unknown mechanism. 31• Using bioinformatics tools, we identified a previously uncharacterized protein in 32 sorghum (Sorghum bicolor), which we named Siliplant1 (Slp1). Silica precipitation 33 activity in vitro, expression profile, and activity in precipitating biosilica in vivo were 34 characterized. 35• Slp1 is a basic protein with seven repeat units rich in proline, lysine, and glutamic acid. 36 A short peptide, repeating five times in the protein precipitated silica in vitro at a 37 biologically relevant silicic acid concentration. Raman and NMR spectroscopies 38 showed that the peptide attached the silica through lysine amine groups, forming a 39 mineral-peptide open structure. We found Slp1 expression in immature leaf and 40 inflorescence tissues. In the immature leaf active silicification zone, Slp1 was localized 41 to the cytoplasm or near cell boundaries of silica cells. It was packed in vesicles and 42 secreted to the paramural space. Transient overexpression of Slp1 in sorghum resulted 43 in ectopic silica deposition in all leaf epidermal cell types. 44 • Our results show that Slp1 precipitates silica in sorghum silica cells. 45 46 47

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Section: Discussionmentioning

confidence: 94%

Siliplant1 (Slp1) protein precipitates silica in sorghum silica cells

Kumar

Adiram-Filiba²,

Blum

et al. 2019

Preprint

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“…It should be noted in this context that synthetic silica/biomolecule composites in general find increasing interest for various reasons. − Solid-state NMR spectroscopy with and without DNP is a favorable method for their characterization. Interestingly, close contact between 1 H nuclei of the ε-carbons of lysine residues in the silaffin-derived so-called PL12 peptide (KAAKLFKPKASK) and 29 Si in silica was detected by 1 H– 29 Si heteronuclear correlation (HETCOR) spectroscopy .…”

Section: Introductionmentioning

confidence: 99%

Interactions of Long-Chain Polyamines with Silica Studied by Molecular Dynamics Simulations and Solid-State NMR Spectroscopy

et al. 2020

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The investigation of molecular interactions between silica phases and organic components is crucial for elucidating the main steps involved in the biosilica mineralization process. In this respect, the structural characterization of the organic/inorganic interface is particularly useful for a deeper understanding of the dominant mechanisms of biomineralization. In this work, we have investigated the interaction of selectively 13C- and 15N-labeled atoms of organic long-chain polyamines (LCPAs) with 29Si-labeled atoms of a silica layer at the molecular level. In particular, silica/LCPA nanocomposites were analyzed by solid-state NMR spectroscopy in combination with all-atom molecular dynamics simulations. Solid-state NMR experiments allow the determination of 29Si–15N and 29Si–13C internuclear distances, providing the parameters for direct verification of atomistic simulations. Our results elucidate the relevant molecular conformations as well as the nature of the interaction between the LCPA and a silica substrate. Specifically, distances and second moments suggest a picture compatible with (i) LCPA completely embedded in the silica phase and (ii) the charged amino groups located in close vicinity of silanol groups.

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“…show that the mineral crystallites formed in HAP•qOPN, like in HAP•umOC 26 and HAP•ON50 29 , have morphology and dimensions similar to that found in apatite platelets in bone, contrarily to HAP•ON29 27,28 , where needle-shaped crystallites are formed. The powder XRD data further corroborate the mild effect of qOPN on apatite crystallite properties compared to either ON29 or umOC.…”

Section: Discussion Influence Of Qopn and Other Non-collagenous Protmentioning

confidence: 54%

“…The existence of such intermediate mineral phase alleviates the stringent transition from a completely disordered hydrated phase to a highly ordered core crystalline phase. ON29 and umOC co-precipitated with the mineral were capable of changing the content of HPO 4 2− ions and water molecules in the hydrated layer as well 26 – 29 . These findings open new questions regarding the capabilities possessed by non-collagenous proteins, for regulating and modifying the mineral phases via organic–inorganic interactions.…”

Section: Biological Activity Of Non-colagenous Proteinsmentioning

confidence: 99%

Osteopontin regulates biomimetic calcium phosphate crystallization from disordered mineral layers covering apatite crystallites

Iline-Vul

Nanda

Mateos

et al. 2020

Sci Rep

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Details of apatite formation and development in bone below the nanometer scale remain enigmatic. Regulation of mineralization was shown to be governed by the activity of non-collagenous proteins with many bone diseases stemming from improper activity of these proteins. Apatite crystal growth inhibition or enhancement is thought to involve direct interaction of these proteins with exposed faces of apatite crystals. However, experimental evidence of the molecular binding events that occur and that allow these proteins to exert their functions are lacking. Moreover, recent high-resolution measurements of apatite crystallites in bone have shown that individual crystallites are covered by a persistent layer of amorphous calcium phosphate. It is therefore unclear whether non-collagenous proteins can interact with the faces of the mineral crystallites directly and what are the consequences of the presence of a disordered mineral layer to their functionality. In this work, the regulatory effect of recombinant osteopontin on biomimetic apatite is shown to produce platelet-shaped apatite crystallites with disordered layers coating them. The protein is also shown to regulate the content and properties of the disordered mineral phase (and sublayers within it). Through solid-state NMR atomic carbon-phosphorous distance measurements, the protein is shown to be located in the disordered phases, reaching out to interact with the surfaces of the crystals only through very few sidechains. These observations suggest that non-phosphorylated osteopontin acts as regulator of the coating mineral layers and exerts its effect on apatite crystal growth processes mostly from afar with a limited number of contact points with the crystal.

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Understanding the roles of functional peptides in designing apatite and silica nanomaterials biomimetically using NMR techniques

Cited by 14 publications

References 56 publications

Siliplant1 (Slp1) protein precipitates silica in sorghum silica cells

Siliplant1 (Slp1) protein precipitates silica in sorghum silica cells

Interactions of Long-Chain Polyamines with Silica Studied by Molecular Dynamics Simulations and Solid-State NMR Spectroscopy

Osteopontin regulates biomimetic calcium phosphate crystallization from disordered mineral layers covering apatite crystallites

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