Understanding the regulation of overwintering diapause molecular mechanisms in Culex pipiens pallens through comparative proteomics

Zhang, Chongxing; Wei, Dongdong; Guo-qing, Shi; Huang, Xiaoli; Peng, Cheng; Liu, Gongzhen; Guo, Xia; Liu, Lijuan; Wang, Huaiwei; Miao, Feng; Gong, Maoqing

doi:10.1038/s41598-019-42961-w

Cited by 41 publications

(21 citation statements)

References 78 publications

(61 reference statements)

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“…At low temperatures, certain proteins are selectively degraded to meet the needs of the cell (Guy et al, 2008;Zhang et al, 2019). In the present study, 20 PRs were identified as being downregulated.…”

Section: Rpsmentioning

confidence: 58%

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

et al. 2020

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Adaptations to low temperature play a critical role in restricting the geographical distribution of insects. Decreasing day lengths and temperatures trigger seasonal cold adaptations in insects. These adaptions include changes in expression at the miRNA, mRNA and protein levels. The rice water weevil (RWW), Lissorhoptrus oryzophilus, introduced from the Mississippi River, is a globally invasive pest of wetland rice that can survive at the northern border of China. To investigate the changes in expression at the protein level in overwintering female RWW adults, 6-plex tandem mass tags (TMTs) were used in overwintering and summer adults. By using a proteome database available for Curculionidae, 1077 proteins were quantified, 183 of which differed significantly between the overwintering and summer samples. To further understand these differentially expressed proteins (DEPs), bioinformatics analyses such as gene ontology (GO) enrichment analyses were performed. DEPs associated with the terms binding, structural molecule activity, catalytic activity, multicellular organismal process, extracellular region, chitin binding, metabolic process, intracellular part and organic cyclic compound binding were altered by selection during winter. The changes in the expression of these proteins suggest that the proteins are important for RWW survival in winter.

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Section: Rpsmentioning

confidence: 58%

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

et al. 2020

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“…Shao et al (2018) documented that the lipid content of the diapausing larvae of D. tabulaeformis gradually decreased during the diapause initiation, remained stable in the diapause maintenance, and reached amounts comparable to those observed in September at the diapause termination. Zhang et al (2019) showed that the overwintering females of Culex pipiens pallens (L.) (Dip: Culicidae) accumulated more lipids during the diapause initiation and maintenance than during the non-diapausing phase. Employing lipids as a source of energy during diapause was also found in some other insects (Han et al, 2008;Behroozi et al, 2012;Bemani et al, 2012;Heydari and Izadi, 2014).…”

Section: Total Body Sugar and Glycogen And Lipid Contentsmentioning

confidence: 99%

Overwintering Physiology and Cold Tolerance of the Sunn Pest, Eurygaster integriceps, an Emphasis on the Role of Cryoprotectants

2020

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As a serious pest of wheat, the Sunn pest, Eurygaster integriceps Puton (Hem.: Scutelleridae), is prevalent in Iran. This pest belongs to univoltine species and tends to estivate and overwinter in high altitudes of nearby mountains as diapausing adults. The economic importance of the crop was attacked by this pest, i.e., wheat led the authors to study the physiological adaptations of these diapausing adults, that is, changes in the supercooling point (SCP), in the accumulation of cryoprotectants, and in the activities of the related enzymes in relation to diapause development. The mean SCP of the diapausing adults was found to be −8 • C. The lowest SCP, i.e., approximately −11 • C, was observed in the middle of diapause, October, when the highest cold hardiness was also interestingly recorded. This finding proposed that SCP depression could be a feasible cold-tolerance strategy for diapausing adults. The sugar content was high in the initiation and at the termination of diapause and was low during diapause maintenance. These sugar reserves were most likely utilized to be converted to glycogen and lipid during diapause maintenance as a survival strategy. The changes in the glycogen and lipid contents were inversely proportional to the changes in the total sugar content. The authors also found that the changes in the glycogen content were directly proportional to those in the low-molecular-weight carbohydrates (e.g., glycerol and trehalose) and in the diapause development. This finding underlined the role of the low-molecularweight carbohydrates, such as the cryoprotectants, in enhancing the cold tolerance of the given insect. In this study, the diapause-associated changes in the activities of α-amylases and proteases were also investigated. The results showed that the enzyme activities were related to diapause development and cold-tolerance enhancement. The highest enzyme activity was observed in September. Since the overwintering adults of the Sunn pest could not tolerate temperatures below their SCPs, they were grouped in the freeze-intolerant species.

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“…However, it is nearly unknown whether Ca 2+ signaling is involved in regulating diapause in animals. More recent research indicated that lots of CaBPs were differentially expressed during diapause in insects and mites, such as H. armigera [56,57], Tetranychus urticae [58], Bombyx mori [59], and Culex pipiens pallens [60], suggesting that Ca 2+ signaling might play a critical role in the diapause regulation of insects and mites. In this study, the 51 putative target unigenes involved in Ca 2+ signaling for 41 DEMs were obtained (Table S10).…”

Section: Discussionmentioning

confidence: 99%

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

Duan¹,

Li²,

Tan³

et al. 2020

Preprint

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Background: MicroRNAs (miRNAs) are a class of small noncoding RNAs of approximately 22 nt in length, which regulate gene expression at the post-transcriptional level. Although the regulatory roles of miRNAs in various physiological processes throughout insect development have been investigated, it is almost unknown about the roles of miRNAs involved in the regulation of diapause in insects.Results: We constructed 12 small RNA libraries from Galeruca daurica adults at different diapause stages: pre-diapause (PD), diapause (D), post-diapause 1 (TD1), and post-diapause 2 (TD2). Using Illumina sequencing, a total of 95.06 million valid reads was obtained, and 230 miRNAs, including 143 conserved and 87 novel miRNAs, were identified from G. daurica. The expression profiles of these miRNAs were assessed across different diapause stages and miRNAs that were highly expressed at different diapause stages were identified. Comparative analysis of read counts indicated that both conserved and novel miRNAs were differently expressed among the four different diapause stages, and the differential expression was validated via qRT-PCR. The 25, 11, 15, 14, 26, and one miRNAs were differentially expressed in D/PD, D/TD1, D/TD2, TD1/PD, TD2/PD, and TD2/TD1, respectively. The KEGG and GO analysis of the predicted target genes suggested the essential roles of miRNAs in the regulation of summer diapause in G. daurica, especially via the juvenile hormone, ribosome, MAPK signaling, mTOR signaling, Ca2+ signaling, and G-protein coupled receptor signaling pathways.Conclusion: Our research results indicate that miRNAs may be involved in the regulation of summer diapause in G. daurica, and these results also provide an important new small RNA genomics resource for further studies on insect diapause.

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Understanding the regulation of overwintering diapause molecular mechanisms in Culex pipiens pallens through comparative proteomics

Cited by 41 publications

References 78 publications

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

Overwintering Physiology and Cold Tolerance of the Sunn Pest, Eurygaster integriceps, an Emphasis on the Role of Cryoprotectants

Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults

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Understanding the regulation of overwintering diapause molecular mechanisms in Culex pipiens pallens through comparative proteomics

Cited by 41 publications

References 78 publications

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

Overwintering Physiology and Cold Tolerance of the Sunn Pest, Eurygaster integriceps, an Emphasis on the Role of Cryoprotectants

Identification and Comparative Profiling of microRNAs&nbsp;at Different Diapause Stages of Galeruca Daurica Adults

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Identification and Comparative Profiling of microRNAs at Different Diapause Stages of Galeruca Daurica Adults