Understanding the origins of UV-induced recombination through manipulation of sister chromatid cohesion

Covo, Shay; Ma, Wenjian; Westmoreland, James W.; Gordenin, Dmitry A.; Resnick, Michael A.

doi:10.4161/cc.21945

Cited by 15 publications

(24 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found previously ( Yin and Petes, 2013 ) that more than half of the recombination events detected in G 1 -synchronized diploids treated with high doses of UV had the pattern of recombination indicative of the repair of a G 1 -associated DSB ( Figure 3B ). In addition, DSBs induced by UV were detected by gel electrophoresis ( Covo et al, 2012 ). Such DSBs could be formed as a consequence of nucleotide-excision-repair (NER) enzymes acting on two very closely-spaced lesions on opposite strands or as the result of expansion of short single-stranded NER-generated gaps on opposite strands by the action of Exo1p.…”

Section: Resultsmentioning

confidence: 99%

High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast

Yin

Dominska

Yim

et al. 2017

eLife

View full text Add to dashboard Cite

In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination.

show abstract

Section: Resultsmentioning

confidence: 99%

High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast

Yin

Dominska

Yim

et al. 2017

eLife

View full text Add to dashboard Cite

show abstract

“…In the Argueso et al study, the gamma ray dose (800 Gray) produces about 250 DSBs/cell. Based on the estimate that 40 J/m 2 of UV results in 5–10 DSBs in G2-synchronized cells [42], we expect only about two DSBs/cell as the result of irradiating G1-synchronized cells with 15 J/m 2 . Since Ty elements, the main target for chromosome rearrangements, represents only a small fraction of the genome (2%), the likelihood of a UV-induced DSB within Ty elements is small, although DSBs located near Ty elements can also contribute to Ty-Ty exchanges [55].…”

Section: Discussionmentioning

confidence: 98%

“…Although UV damage is generally regarded as an agent that produces DNA nicks rather than DSBs, a gel-based detection of the conversion of a circular chromosome to a linear chromosome indicated that a dose of 40 J/m 2 produces 5 to 10 DSBs in G2-synchronized cells [42]. There are several related mechanisms by which NER could produce a DSB in G1 cells.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide High-Resolution Mapping of UV-Induced Mitotic Recombination Events in Saccharomyces cerevisiae

2013

View full text Add to dashboard Cite

In the yeast Saccharomyces cerevisiae and most other eukaryotes, mitotic recombination is important for the repair of double-stranded DNA breaks (DSBs). Mitotic recombination between homologous chromosomes can result in loss of heterozygosity (LOH). In this study, LOH events induced by ultraviolet (UV) light are mapped throughout the genome to a resolution of about 1 kb using single-nucleotide polymorphism (SNP) microarrays. UV doses that have little effect on the viability of diploid cells stimulate crossovers more than 1000-fold in wild-type cells. In addition, UV stimulates recombination in G1-synchronized cells about 10-fold more efficiently than in G2-synchronized cells. Importantly, at high doses of UV, most conversion events reflect the repair of two sister chromatids that are broken at approximately the same position whereas at low doses, most conversion events reflect the repair of a single broken chromatid. Genome-wide mapping of about 380 unselected crossovers, break-induced replication (BIR) events, and gene conversions shows that UV-induced recombination events occur throughout the genome without pronounced hotspots, although the ribosomal RNA gene cluster has a significantly lower frequency of crossovers.

show abstract

“…Nearly all (over 99%) of the copper resistant colonies were able to grow again on copper plates following replica-plating. Details about inter chromosome recombination assay are found in previous work [14] – [16] .…”

Section: Methodsmentioning

confidence: 99%

“…We have shown that several hypomorphic mutations in cohesin (the sister chromatid cohesion complex) increase the rate of allelic recombination and chromosome gain [14] – [16] . However, the functional role of Chk1-mediated phosphorylation of cohesin remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

Suppression of Allelic Recombination and Aneuploidy by Cohesin Is Independent of Chk1 in Saccharomyces cerevisiae

et al. 2014

Self Cite

View full text Add to dashboard Cite

Sister chromatid cohesion (SCC), which is established during DNA replication, ensures genome stability. Establishment of SCC is inhibited in G2. However, this inhibition is relived and SCC is established as a response to DNA damage, a process known as Damage Induced Cohesion (DIC). In yeast, Chk1, which is a kinase that functions in DNA damage signal transduction, is considered an activator of SCC through DIC. Nonetheless, here we show that, unlike SCC mutations, loss of CHK1 did not increase spontaneous or damage-induced allelic recombination or aneuploidy. We suggest that Chk1 has a redundant role in the control of DIC or that DIC is redundant for maintaining genome stability.

show abstract

Understanding the origins of UV-induced recombination through manipulation of sister chromatid cohesion

Cited by 15 publications

References 49 publications

High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast

High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast

Genome-Wide High-Resolution Mapping of UV-Induced Mitotic Recombination Events in Saccharomyces cerevisiae

Suppression of Allelic Recombination and Aneuploidy by Cohesin Is Independent of Chk1 in Saccharomyces cerevisiae

Contact Info

Product

Resources

About