Understanding the influence of heavy water stress on the physiology of Salmonella Typhimurium

Pant, Indu; Shashidhar, Ravindranath

doi:10.1016/j.apradiso.2019.108990

Cited by 1 publication

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“…The effect of H/D substitution on the kinetics of chemical reactions is well-known, and has been extensively studied and applied, for instance to study reaction mechanisms and to monitor protein folding . Interestingly, recent work shows that the kinetic effects induced by substituting D 2 O for H 2 O might also be useful for biomedical purposes: epithelial cells grown in a medium containing 45% D 2 O show significantly reduced migration and proliferation rates (Figure ), and a similar slowdown in dynamics was observed in other cells, − an effect that might find use for the storage of biological materials such as organs, or for anticancer treatment …”

Section: Introductionmentioning

confidence: 99%

D₂O as an Imperfect Replacement for H₂O: Problem or Opportunity for Protein Research?

Giubertoni,

Bonn,

Woutersen

2023

J. Phys. Chem. B

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D 2 O is commonly used as a solvent instead of H 2 O in spectroscopic studies of proteins, in particular, in infrared and nuclear-magnetic-resonance spectroscopy. D 2 O is chemically equivalent to H 2 O, and the differences, particularly in hydrogen-bond strength, are often ignored. However, replacing solvent water with D 2 O can affect not only the kinetics but also the structure and stability of biomolecules. Recent experiments have shown that even the mesoscopic structures and the elastic properties of biomolecular assemblies, such as amyloids and protein networks, can be very different in D 2 O and H 2 O. We discuss these findings, which probably are just the tip of the iceberg, and which seem to call for obtaining a better understanding of the H 2 O/D 2 O-isotope effect on water−water and water−protein interactions. Such improved understanding may change the differences between H 2 O and D 2 O as biomolecular solvents from an elephant in the room to an opportunity for protein research.

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Section: Introductionmentioning

confidence: 99%

D₂O as an Imperfect Replacement for H₂O: Problem or Opportunity for Protein Research?

Giubertoni,

Bonn,

Woutersen

2023

J. Phys. Chem. B

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show abstract

Understanding the influence of heavy water stress on the physiology of Salmonella Typhimurium

Cited by 1 publication

References 35 publications

D₂O as an Imperfect Replacement for H₂O: Problem or Opportunity for Protein Research?

D₂O as an Imperfect Replacement for H₂O: Problem or Opportunity for Protein Research?

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Understanding the influence of heavy water stress on the physiology of Salmonella Typhimurium

Cited by 1 publication

References 35 publications

D2O as an Imperfect Replacement for H2O: Problem or Opportunity for Protein Research?

D2O as an Imperfect Replacement for H2O: Problem or Opportunity for Protein Research?

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D₂O as an Imperfect Replacement for H₂O: Problem or Opportunity for Protein Research?

D₂O as an Imperfect Replacement for H₂O: Problem or Opportunity for Protein Research?