Understanding the genetic relationships between Indonesian bambara groundnut landraces and investigating their origins

Abstract

A total of 170 bambara groundnut (Vigna subterranea) accessions were evaluated using both simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers generated using genotyping-by-sequencing (GbS), of which 56 accessions were collected from West and East Java. Principal coordinate analysis (PCoA), population structure, and cluster analysis suggest that the East Java accessions could be a result of the introduction of selected West Java accessions. In addition, the current Indonesian accessions were likely introduced from Southern Africa, which would have produced a very marked founding effect such that these accessions present only a fraction of the genetic variability that exists within this species.

Root Foraging Capacity in Bambara Groundnut (Vigna Subterranea (L.) Verdc.) Core Parental Lines Depends on the Root System Architecture during the Pre-Flowering Stage

“…Differences in root architecture among genotypes from hot-dry and humid-wet regions suggest an adaptive response of bambara groundnut for soil resource capture by means of an improved foraging capacity of the root system in the hot-dry region sourced genotypes [53,54]. Interestingly, Gresik was derived from an introduction into Indonesia from Africa not so long ago and most likely from Southern and East Africa [55]. Practically, it would make sense that Gresik would need to have adapted quickly into a costlier highly branching root system in the shallow topsoil layer, given the conditions in Indonesia.…”

Genetics and breeding for climate change in Orphan crops

“…More recently, DArT has been combined with NGS in a procedure called DArT-sequencing (DArT-seq) (Sansaloni et al 2011) that enables high throughput genotyping for rapid SNP discovery in many orphan crops. DArT sequencing is now being used in the characterization of many climate-resilient orphan crops including Bambara groundnut (Redjeki et al 2020), finger millet (Dida et al 2020 Aside from DArT-sequencing, other restriction-associated DNA sequencing (RADseq) (Davey et al 2011) genotyping methods including genotyping-by-sequencing (GBS) (Elshire et al 2011) have also been exploited in the characterization and linkage mapping of orphan crops, especially where a reference genome is available as was done in white Guinea yam (Dioscorea rotundata) (Tamiru et al 2017). These sequence-based genotyping platforms are the future of genotyping in all crops including orphan crops with no reference genomes.…”

“…More recently, DArT has been combined with NGS in a procedure called DArT-sequencing (DArT-seq) (Sansaloni et al 2011 ) that enables high throughput genotyping for rapid SNP discovery in many orphan crops. DArT sequencing is now being used in the characterization of many climate-resilient orphan crops including Bambara groundnut (Redjeki et al 2020 ), finger millet (Dida et al 2020 ), Kersting’s groundnut ( Kerstingiella geocarpa ) (Kafoutchoni et al 2020 ), lupin ( Lupinus albus ) (Raman et al 2014) and grass pea (Almeida et al 2016 ).…”

Potential of genomics for the improvement of underutilized legumes in sub‐Saharan Africa

“…The majority of wild relatives were grouped with West African accessions (Paliwal et al, 2019), confirming that it is an indigenous West African crop. A genetic relationship study was carried out for 56 Indonesian Bambara Groundnut with 114 diverse accessions from Africa to investigate their origin with SSR and SNP markers by Redjeki et al (2020). They suggested East Java Bambara groundnut lines could be introduced from West Java materials.…”