Understanding the genetic relationships and breeding patterns of Sri Lankan tea cultivars with genomic and EST-SSR markers

Karunarathna, K. H. T.; Mewan, K.M.; Weerasena, O.V.D.S.J.; Perera, S. A. C. N.; Edirisinghe, E.N.U.; Jayasoma, AA

doi:10.1016/j.scienta.2018.05.051

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…In this study, the mean Nei's gene diversity (GD, 0.792) across 21 SSR markers was higher than other studies; 0.652 in 280 tea accessions using 23 SSR markers [7], 0.640 in 450 tea accessions using 96 EST-SSR marker [22], 0.543 in 185 Chinese tea cultivars using 48 SSR markers [13], and 0.680 in 64 Sri Lankan tea cultivars using 33 EST-or genomic-SSR markers [3]. The gene diversity of a locus, also known expected heterozygosity, is a fundamental measure of genetic variation in a population, and describes the proportion of heterozygosis expected under Hardy-Weinberg equilibrium [36].…”

Section: Discussioncontrasting

confidence: 69%

“…Previous studies performed the analysis of genetic diversity of different tea accessions using molecular markers like RFLP, RAPD, and SSR [3,5,21,22]. In addition, the STRUCTURE software was used to analyze the population structure of tea germplasm [2,3,13,22]. To analyze the genetic diversity and population structure of Korean tea accessions, 21 SSR markers and DAPC analysis were used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Tea (Camellia sinensis (L.) O. Kuntze, 2n = 2x = 30) is one of the most popular non-alcoholic beverages worldwide, and is consumed by approximately 70% of the world's population for its refreshing taste, attractive aroma, therapeutic uses, and mildly stimulating properties [1]. It is an economically important tree crop, grown in over 52 countries in Asia, Africa, and South America [2,3]. The tea is a woody ever-green perennial plant and recorded to be native to Yunnan and Sichuan provinces in China and the northern part of Myanmar [4].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, they offer a possibility to observe the genome directly and thus eliminate the shortcomings inherent in a phenotype observation [15]. In previous studies, genetic diversity, discrimination and differentiation of tea germplasms have been assessed using different DNA markers such as restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), and simple sequence repeat (SSR) [3,5,7,[11][12][13][14][15][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Diversity of Tea Germplasm for Its Management and Sustainable Use in Korea Genebank

Lee

Raveendar

et al. 2019

Forests

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Tea (Camellia sinensis (L.) O. Kuntze) is cultivated in many developing Asian, African, and South American countries, and is the most widely consumed beverage in the world. It is of critical importance to understand the genetic diversity and population structure of tea germplasm for effective collection, conservation, and utilization. In this study, 410 tea accessions collected from South Korea were analyzed using 21 simple sequence repeat (SSR) markers. Among 410 tea accessions, 85.4% (350 accessions) were collected from Jeollanam-do. A total of 286 alleles were observed, and the genetic diversity and evenness were estimated to be on average 0.79 and 0.61, respectively, across all the tested samples. Using discriminant analysis of principal components, four clusters were detected in 410 tea accessions. Among them, cluster 1 showed a higher frequency of rare alleles (less than 1%). Using the calculation of the index of association and rbaD value, each cluster showed a clonal mode of reproduction. The result of analysis of molecular variance (AMOVA) showed that most of the variation observed was within populations (99%) rather than among populations (1%). The present study revealed the presence of lower diversity and simpler population structure in Korean tea germplasms. Consequently, more attention should be focused on collecting and conserving the new tea individuals to broaden genetic variation of new cultivars in future breeding of the tea plant.

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Section: Discussioncontrasting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Genetic Diversity of Tea Germplasm for Its Management and Sustainable Use in Korea Genebank

Lee

Raveendar

et al. 2019

Forests

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“…An understanding of morphological (Piyasundara et al, 2009), biochemical (Kottawa-Arachchi et al, 2013;2014) and molecular (Mevan et al, 2005;Karunarathna et al, 2018) diversity among the Sri Lankan tea germplasm accessions is important if the best use is to be extracted in plant improvement programs. Meanwhile, the generated information of recent studies on floral diversity and metabolite profiling of tea germplasm revealed that results could be effectively used in choosing accessions of desired traits (Punyasiri et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Recent progress of intra-specific hybridization of tea (<em>Camellia sinensis,</em> (L.) O. Kuntze) in Sri Lanka

2019

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Tea germplasm accessions are the most valuable material for tea breeding. However its utilization in the past was limited due to lack of available information. Recently generated information on genetic structure and metabolite profiles of the germplasm were used in selecting parents for hybridization programmes from 2012 to 2016. The main focus of the programme was to make crosses between three taxa of tea viz. Camellia. sinensis var. assamica (Assam type), C. sinensis var. sinensis (China type) and C. sinensis spp. lasiocalyx (Cambod type). Pollinated buds were closelymonitored and the number of crosses, number of fruits, number of seeds and reproductive parameters were recorded. A total of 3978 crosses (2405 direct crosses and 1573 reciprocal crosses) were made and 974 seeds were obtained from 595 fruits. Percentages of fruit set and seed set varied significantly among different families. Irrespective of the intersubspecies groups, the percentage of seed germination was above 50% and was not significantly-different. Cross compatibility between taxa in terms of percentage of fruit set ranged from 4.6% to 25.6% and varied significantly. Among the inter-subspecies crosses the Assam varieties were found to be the best as female parent. The generated information would be useful for selection of parents and tea improvement programmes in the future.

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Genomic Variation and Adaptative Evolution of Tea Plants

Tong,

Wu,

Wang

et al. 2024

Concepts and Strategies in Plant Sciences

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Understanding the genetic relationships and breeding patterns of Sri Lankan tea cultivars with genomic and EST-SSR markers

Cited by 14 publications

References 17 publications

Assessment of Genetic Diversity of Tea Germplasm for Its Management and Sustainable Use in Korea Genebank

Assessment of Genetic Diversity of Tea Germplasm for Its Management and Sustainable Use in Korea Genebank

Recent progress of intra-specific hybridization of tea (<em>Camellia sinensis,</em> (L.) O. Kuntze) in Sri Lanka

Genomic Variation and Adaptative Evolution of Tea Plants

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