Understanding the functional relevance of oral neutrophils, phenotype and properties in OSCC

Lenka, Sudhansubala; Bhola, Rajesh Kumar; Varanasi, Pavankumar R; Bhuyan, Sanat Kumar; Bhuyan, Ruchi

doi:10.1007/s12032-023-02010-z

Cited by 4 publications

(2 citation statements)

References 62 publications

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“…Macrophages activation plays a key role in the pathogenesis of several diseases (9). The polarization of macrophages into M1 (proinflammatory) and M2 (anti-inflammatory) is influenced by interferon (IFN)-g or lipopolysaccharides (M1) or by Th2 cytokines such as IL-10 (M2) (9). M2 macrophages contribute to wound healing and tissue repair, but are also tumor promoting (9).…”

Section: Introductionmentioning

confidence: 99%

“…The polarization of macrophages into M1 (proinflammatory) and M2 (anti-inflammatory) is influenced by interferon (IFN)-g or lipopolysaccharides (M1) or by Th2 cytokines such as IL-10 (M2) (9). M2 macrophages contribute to wound healing and tissue repair, but are also tumor promoting (9). M2 shifting is associated with IL-8 production, and high IL-8 expression is associated with downregulated antigen presentation (10,11).…”

Section: Introductionmentioning

confidence: 99%

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Does surgery affect systemic immune response? a perioperative analysis of TGF-β, IL-8 and CD45RO

Trumet,

Ries,

Ivenz

et al. 2023

Front. Oncol.

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BackgroundThe options of (neo-)adjuvant immunotherapy in addition to surgery in the treatment of oral squamous cell carcinoma (OSCC) are steadily increasing, but patients do not always respond to therapy as intended. The objectives of this study were to investigate the systemic perioperative course of the biomarkers CD45RO, TGF-β, and IL-8 in non-tumor-related minor and tumor-related major maxillofacial surgery and to perform association analyses with demographic and histomorphologic parameters. A deeper understanding of surgery-related changes in various of different immune biomarkers could help to better understand the immunologic consequences of surgery which could influence immunotherapeutic protocols.MethodsPeripheral whole blood from 38 patients was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) at five different timepoints before and after maxillofacial surgery to detect changes in mRNA expression of the biomarkers TGF-β, IL-8 and CD45RO. All patients underwent general anesthesia to undergo either resection and free flap reconstruction for OSCC or minor maxillofacial surgery (controls). Statistical analysis was done using Mann-Whitney-U test, Wilcoxon test, and Spearman’s correlation.ResultsCompared to the preoperative expression, there was a significant postoperative downregulation of CD45RO, TGF-β and IL-8 until the 4th postoperative day (p ≤ 0.003) in OSCC patients. For TGF-β and IL-8, the reduction in expression was significant (p ≤ 0.004) compared to controls. By postoperative day 10, all analyzed parameters converged to baseline levels. Only CD45RO still showed a significant downregulation (p=0.024). Spearman analysis revealed a significant correlation between increased duration of surgery and perioperative reduction in peripheral blood expression of CD45RO, TGF-β and IL-8 (p ≤ 0.004). Perioperative changes in TGF-β and PD-L1 expression were shown to be not correlated. Preoperative TGF-β expression was significantly lower in patients with lymph node metastases (p=0.014).ConclusionWith regard to the analyzed parameters, major oncologic head-and-neck surgery does not seem to have long-lasting systemic immunologic effects. Reduced CD45RO might be an expression of transient systemic immunosuppression in response to major surgery. The association of duration of surgery with expression changes of immunologic markers supports efforts to keep the duration of surgery as short as possible. As perioperative TGF-β and PD-L1 expression changes are not associated, these results support further investigation of a combined perioperative anti-PD-1 and anti-TGF-β immunotherapy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Does surgery affect systemic immune response? a perioperative analysis of TGF-β, IL-8 and CD45RO

Trumet,

Ries,

Ivenz

et al. 2023

Front. Oncol.

View full text Add to dashboard Cite

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Deregulation of cytokine affecting oral neutrophil subsets in oral cancer

Lenka,

Bhuyan,

Bhuyan

2023

Med Oncol

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Inter- and intra-tumor heterogeneous immune presentation in primary uterine carcinosarcoma determined by digital spatial profiling

Li,

Lin,

Chen

et al. 2024

Preprint

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Uterine carcinosarcoma (UCS) is a rare gynecologic cancer with unfavorable prognosis. Its biphasic histologic components are known to associated with outcomes. Dissecting the tumor heterogeneity within UCS is crucial to discover molecular biomarkers for patient stratification. The aim of this study was to utilize spatial profiling technology to analyze immune presentation in different histological components within UCS tumors. The study included 23 UCS patients whose tumor sections were subjected to nanoString GeoMx Digital Spatial Profiling (DSP) to profile 30 immune-related proteins in 491 segments. We identified distinct cluster-labeled subtypes showing varied immune marker expressions. The immune presentations within the carcinoma and sarcoma components showed significant differences. Markers indicating M2 macrophages (CD45+CD163+) and epithelial-mesenchymal transition (EMT) (SMA, fibronectin) were highly expressed in the sarcoma components. The immune presentation subtypes within each histological component also differed between patients with recurrence in 5 years and without recurrence over 5 years. The cluster-labeled subtype with high Neutrophil/PMN-MDSC marker expression (CD66b) was associated with longer survival, whereas the subtype with high NK cell marker expressions (CD56) was linked to poor prognosis. Our result suggested that immune features within the carcinoma component of UCS tumors would determine patient outcomes and should be considered as a strategy for patient stratification.

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Understanding the functional relevance of oral neutrophils, phenotype and properties in OSCC

Cited by 4 publications

References 62 publications

Does surgery affect systemic immune response? a perioperative analysis of TGF-β, IL-8 and CD45RO

Does surgery affect systemic immune response? a perioperative analysis of TGF-β, IL-8 and CD45RO

Deregulation of cytokine affecting oral neutrophil subsets in oral cancer

Inter- and intra-tumor heterogeneous immune presentation in primary uterine carcinosarcoma determined by digital spatial profiling

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