Understanding the diversity of sex steroid action

Poutanen, Matti

doi:10.1530/joe-11-0414

Cited by 3 publications

(2 citation statements)

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“…We thus tested the effect of E2 and T on lipid secretion by Caco-2/TC7 cells and found that, while E2 had no effect, T led to an increase of TAG secretion. In humans, sex steroids are known to exert profound and complex effects including on lipid metabolism [64], [65], [66]. However, data from literature on the effect of T on serum lipid levels are contradictory [67], [68].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Lipid Droplets from Caco-2/TC7 Enterocytes Identifies Novel Modulators of Lipid Secretion

et al. 2013

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In enterocytes, the dynamic accumulation and depletion of triacylglycerol (TAG) in lipid droplets (LD) during fat absorption suggests that cytosolic LD-associated TAG contribute to TAG-rich lipoprotein (TRL) production. To get insight into the mechanisms controlling the storage/secretion balance of TAG, we used as a tool hepatitis C virus core protein, which localizes onto LDs, and thus may modify their protein coat and decrease TRL secretion. We compared the proteome of LD fractions isolated from Caco-2/TC7 enterocytes expressing or not hepatitis C virus core protein by a differential proteomic approach (isobaric tag for relative and absolute quantitation (iTRAQ) labeling coupled with liquid chromatography and tandem mass spectrometry). We identified 42 proteins, 21 being involved in lipid metabolism. Perilipin-2/ADRP, which is suggested to stabilize long term-stored TAG, was enriched in LD fractions isolated from Caco-2/TC7 expressing core protein while perilipin-3/TIP47, which is involved in LD synthesis from newly synthesized TAG, was decreased. Endoplasmic reticulum-associated proteins were strongly decreased, suggesting reduced interactions between LD and endoplasmic reticulum, where TRL assembly occurs. For the first time, we show that 17β-hydroxysteroid dehydrogenase 2 (DHB2), which catalyzes the conversion of 17-keto to 17 β-hydroxysteroids and which was the most highly enriched protein in core expressing cells, is localized to LD and interferes with TAG secretion, probably through its capacity to inactivate testosterone. Overall, we identified potential new players of lipid droplet dynamics, which may be involved in the balance between lipid storage and secretion, and may be altered in enterocytes in pathological conditions such as insulin resistance, type II diabetes and obesity.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Lipid Droplets from Caco-2/TC7 Enterocytes Identifies Novel Modulators of Lipid Secretion

et al. 2013

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“…Neuroprotekcyjne działanie estrogenów polega na zmniejszaniu stresu oksydacyjnego, promowaniu formowania nowych neuronów i synaps oraz zdolności remodelowania i naprawy struktur mózgu oraz jego uszkodzeń [2,3].…”

Section: Is It Possible To Prevent Female Brain Aging and Alzheimer'sunclassified

Is it possible to prevent female brain aging and Alzheimer’s disease?

Krzyczkowska-Sendrakowska¹

2013

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StreszczenieWszystkie struktury i funkcje mózgu są estrogenowrażliwe. Estrogeny mają wpływ na neuroprzekaźniki i neurony oraz działają neuroprotekcyjnie. Regulują system bioenergetyczny mózgu, metabolizm glukozy, proces fosforylacji oksydacyjnej oraz mają wpływ na substraty transporterów glukozy. Niedobór estrogenów prowadzi do stanu hipometabolicznego mózgu, bowiem zmniejsza się metabolizm glukozy. Estrogeny podtrzymując w mózgu proces wykorzystania energii z glukozy, chronią go przed wykorzystywaniem innych źródeł energii jak lipidy, co sprzyja rozwojowi choroby Alzheimera. Estrogeny hamują syntezę amyloidu i białka tau oraz zwiększają aktywność dopaminy, zmniejszając ryzyko choroby Alzheimera. Istnieje krytyczny okres rozpoczęcia terapii hormonalnej, który zapewnia redukcję ryzyka choroby Alzheimera -rozpoczęcie terapii do 5 lat po menopauzie zmniejsza ryzyko o 30% i utrzymuje na obniżonym poziomie, jeśli leczenie hormonalne jest stosowane 10 lat lub dłużej.Słowa kluczowe: mózg, starzenie się, choroba Alzheimera. SummaryAll brain's structures and functions are estrogen sensitive. Estrogens do influence neurotransmitters and neurons. They are also neuroprotective. Estrogens regulate the brain's bio-energetic system by regulating glucose metabolism, and the process of oxidative phosphorylation. They influence the transporter's substrates. Shortage of estrogens, by lowering glucose metabolism, leads to the brain's hypometabolic state. Estrogens support the process of brain drawing energy from glucose, which prevents brain from drawing energy from other sources such as lipids. Such metabolic status favors the development of Alzheimer's disease. Estrogens limit synthesis of the amyloid and protein tau and enhance activity of the dopamine, what reduces the risk of Alzheimer's disease. There is a critical time when the hormonal therapy should be started in order to reduce the risk of Alzheimer's disease. Starting the therapy within 5 years after menopause reduces the risk of Alzheimer's disease by 30%. The risk will be limited if the hormonal therapy is used for ten years or longer.Key words: brain, aging, Alzheimer's disease.Czy mo¿na przeciwdzia³aae starzeniu siê kobiecego mózgu i rozwojowi choroby Alzheimera? Is it possible to prevent female brain aging and Alzheimer's disease?Magdalena Krzyczkowska-Sendrakowska Klinika Endokrynologii Ginekologicznej Szpitala Uniwersyteckiego w KrakowiePrzegląd Menopauzalny 2013; 1: 13-14 DOI: 10.5114/pm.2013.33415 Estrogeny odgrywają ogromną rolę w całym organizmie kobiety. Działają ochronnie na układ krążenia, układ kostno-stawowy, na tkankę łączną i skórę. Dla funkcji mózgu estrogeny są nieodzowne, a wszystkie struktury mózgu są estrogenowrażliwe. Estrogeny mogą wywierać na tkankę mózgową działanie bezpośrednie (niegenomowe) poprzez oddziaływanie z receptorami błonowymi lub pośrednie (genomowe) przez receptory jądrowe. W różnych neuronach stwierdza się różny poziom zawartości receptorów estrogenowych i aromatazy.Mózg ludzki stanowi 2% masy ciała, zużywa 15% wyrzutu serca, 2...

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Position paper: The membrane estrogen receptor GPER – Clues and questions

Barton

2012

Steroids

102

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Understanding the diversity of sex steroid action

Cited by 3 publications

References 11 publications

Proteomic Analysis of Lipid Droplets from Caco-2/TC7 Enterocytes Identifies Novel Modulators of Lipid Secretion

Proteomic Analysis of Lipid Droplets from Caco-2/TC7 Enterocytes Identifies Novel Modulators of Lipid Secretion

Is it possible to prevent female brain aging and Alzheimer’s disease?

Position paper: The membrane estrogen receptor GPER – Clues and questions

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