Understanding the diversity and reproductive biology of banana – for improvement through basic research

Uma, S.; Arun, Kanchana

doi:10.17660/actahortic.2016.1114.1

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…The results of expression studies inferred that (i) Majority of the variations were observed between 24 h after pollination (P24) and UnP in CVR and PL but not in P48. This is in line to our earlier findings that male gametes reach the ovule within 24 h after artificial pollination 63 and so we suggested that ovary sampling around 24 h after pollination is optimum for seed set studies in AA genome accessions of Musa spp. (ii) Similar expression pattern of MaAGL8, MaMADS16, MaRGA1, MaBAM1 and MaEXPA1 in P24 samples of CVR and C4 whereas except MaBAM1 , similar pattern of expression were observed in UnP of CVR and PL.…”

Section: Discussionsupporting

confidence: 92%

Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein–protein interaction network

Backiyarani

Rajendran

Simeon

et al. 2021

Sci Rep

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Banana, one of the most important staple fruit among global consumers is highly sterile owing to natural parthenocarpy. Identification of genetic factors responsible for parthenocarpy would facilitate the conventional breeders to improve the seeded accessions. We have constructed Protein–protein interaction (PPI) network through mining differentially expressed genes and the genes used for transgenic studies with respect to parthenocarpy. Based on the topological and pathway enrichment analysis of proteins in PPI network, 12 candidate genes were shortlisted. By further validating these candidate genes in seeded and seedless accession of Musa spp. we put forward MaAGL8, MaMADS16, MaGH3.8, MaMADS29, MaRGA1, MaEXPA1, MaGID1C, MaHK2 and MaBAM1 as possible target genes in the study of natural parthenocarpy. In contrary, expression profile of MaACLB-2 and MaZEP is anticipated to highlight the difference in artificially induced and natural parthenocarpy. By exploring the PPI of validated genes from the network, we postulated a putative pathway that bring insights into the significance of cytokinin mediated CLAVATA(CLV)–WUSHEL(WUS) signaling pathway in addition to gibberellin mediated auxin signaling in parthenocarpy. Our analysis is the first attempt to identify candidate genes and to hypothesize a putative mechanism that bridges the gaps in understanding natural parthenocarpy through PPI network.

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Section: Discussionsupporting

confidence: 92%

Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein–protein interaction network

Backiyarani

Rajendran

Simeon

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The expression study revealed that irrespective of the cultivars, majority of the variations was observed between the seeded and seedless accessions at 24 hrs after pollination (P24). This con rmed that ovary sampling around 24 hrs after pollination is optimum for seed set studies in Musa spp which could be well correlated to our earlier ndings that male gametes reach the ovule within 24 hrs after arti cial pollination 60 . Genes belonging to MADS family namely MADS16, MADS29, AGL8, LFY were repeatedly found in all the three centrality measures as well as in cluster analysis and thus ranked as key genes.…”

Section: Discussionsupporting

confidence: 85%

Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein-protein interaction network

Backiyarani

Rajendran

Simeon

et al. 2021

Preprint

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Banana, one of the most important staple, delicious fruit among global consumers is highly sterile owing to natural parthenocarpy. Identification of genetic factors responsible for parthenocarpy would facilitate the conventional breeders to improve the seeded accessions. We have constructed Protein-protein interaction (PPI) network through mining differentially expressed genes and the genes used for transgenic studies with respect to parthenocarpy. Based on the topological and pathway enrichment analysis of proteins in PPI network, 12 candidate genes were shortlisted. By exploring the PPI of candidate genes from the putative network, we postulated a putative pathway that bring insights into the significance of cytokinin mediated CLV-WUSHEL signaling pathway in addition to gibberellin mediated auxin signaling pathway in parthenocarpy. Further validation of candidate genes in seeded and seedless accession of Musa spp using qRT-PCR put forward AGL8, MADS16, IAA (GH3.8), RGA1, EXPA1, GID1C, HK2 and BAM1 as possible target genes in natural parthenocarpy. In contrary, expression profile of ACLB-2 and ZEP is anticipated to highlight the difference in artificially induced and natural parthenocarpy. Our analysis is the first attempt to identify candidate genes and to hypothesize a putative mechanism that bridges the gaps in understanding natural parthenocarpy through protein-protein interaction network.

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“…Empty stylar canals have a star shape but the tissue is able to expand to accommodate the pollen tubes and then has a more rounded appearance. Not all pollen tubes that enter the transmitting tissue make it to the base of the style and into the locules, as also observed in Musa (Dodds, 1945;Uma and Arun, 2016). The transmitting tissue may therefore act as a point of selection for pollen tubes.…”

Section: The Stylementioning

confidence: 92%

“…To achieve this, male parents need to be planted earlier than female parents when establishing crossing blocks. Pollen preservation can also be explored to address the temporal and spatial separation of male and female flowers but protocols are yet to be established for pollen storage in bananas (Uma and Arun, 2016). Pollination can be carried out all year round depending on the availability of male and female flowers.…”

Section: Pollination Techniquesmentioning

confidence: 99%

Overcoming the fertility crisis in bananas (Musa spp.)

Amah¹,

Turner²,

Gibbs³

et al. 2020

Burleigh Dodds Series in Agricultural Science

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Understanding the diversity and reproductive biology of banana – for improvement through basic research

Cited by 4 publications

References 40 publications

Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein–protein interaction network

Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein–protein interaction network

Decoding the molecular mechanism of parthenocarpy in Musa spp. through protein-protein interaction network

Overcoming the fertility crisis in bananas (Musa spp.)

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