Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: Modeling, measurements, and implications

Drezek, Rebekah A.; Sokolov, Konstantin; Utzinger, Urs; Boiko, Iouri; Malpica, Anaís; Follen, Michele; Richards‐Kortum, Rebecca

doi:10.1117/1.1413209

Cited by 262 publications

(208 citation statements)

References 27 publications

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“…The axial and radial grid sizes were set at 5 μm, and the numbers of axial and radial grids were set at 900 and 50, respectively to define the region of interest within the epithelial tissue model. Fluorescence was simulated in the homogeneous medium for two different sets of optical properties, which correspond to those of the epithelial and stromal layers in epithelial tissues at an excitation-emission wavelength pair of 410-460 nm ( Table 2, set 1) and were obtained from the publication by Drezek et al (32). Optical properties at 410-460 nm were used because fluorescence spectra at an excitation wavelength of 410 nm (measured with the unshielded probe geometry) were found to be most useful for the diagnosis of dysplasia (see Results section).…”

Section: Monte Carlo Simulationsmentioning

confidence: 99%

“…The thickness of the epithelial layer and that of the stromal layer were fixed at 450 μm and 2050 μm, respectively. A thickness of 450 μm was chosen for the epithelial layer to approximate the average thickness of the normal human cervical epithelium (4,6,8,32). The thickness of the stromal layer was set at 2050 μm to represent an infinitely thick tissue.…”

Section: Monte Carlo Simulationsmentioning

confidence: 99%

“…The axial and radial grid sizes were set at 10 μm and 100 μm, respectively, and the numbers of axial and radial grids were set at 250 and 50, respectively to define the region of interest within the epithelial tissue model. Optical properties corresponding to an excitation-emission wavelength pair of 460-520 nm were designated for the theoretical tissue model ( Table 2, set 2), and were obtained from the publication by Drezek et al (32). Optical property set 2 was used in the fluorescence simulations in order to compare the fluorescence detected with the angled illumination probe to previously proposed approaches for depth-resolved fluorescence detection from epithelial tissues, which include the multi-distance and variable aperture approaches (16).…”

Section: Monte Carlo Simulationsmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

et al. 2004

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Background and Objective-The first objective of this study was to evaluate the performance of fluorescence spectroscopy for diagnosing pre-cancers in stratified squamous epithelial tissues in vivo using two different probe geometries with (1) overlapping vs. (2) non-overlapping illumination and collection areas on the tissue surface. Probe (1) and probe (2) are preferentially sensitive to the fluorescence originating from the tissue surface and sub-surface tissue depths, respectively. The second objective was to design a novel, angled illumination fiber-optic probe to maximally exploit the depth-dependent fluorescence properties of epithelial tissues.

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Section: Monte Carlo Simulationsmentioning

confidence: 99%

Section: Monte Carlo Simulationsmentioning

confidence: 99%

Section: Monte Carlo Simulationsmentioning

confidence: 99%

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Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

et al. 2004

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“…Fluorescence spectroscopy has high diagnostic accuracy when the results of NADH and collagen fluorescence are considered together with only 4.34 % false negative. However, false positive could not be calculated due to lack of control a False positive rates could not be calculated in the case of fluorescence spectroscopy as tissue from normal subjects could not be studied which is non-fluorescent [6]. In this study, significant epithelial fluorescence was observed in most of the fresh tissue sections and the fluorescence intensity increased at 460 nm excitation in dysplastic samples relative to normal samples from the same patient.…”

Section: Discussionmentioning

confidence: 79%

Fluorescence Spectroscopy: A New Approach in Cervical Cancer

Pandey

Pradhan

Agarwal

et al. 2012

J Obstet Gynecol India

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Objective To study the diagnostic potential of fluorescence spectroscopy and its comparison with different screening methods, including Pap smear and colposcopy, in detecting early cervical neoplasia. Method The study was conducted on patients with gynecological complaints. A full gynecological workup of the patients was done along with Pap smear and colposcopy. Cervical biopsy was done in suspected cases and fresh tissue was sent to IIT for spectroscopy. Result There is a definite increase in NADH fluorescence (67.4 %) and a decrease in collagen fluorescence (74 %) in dysplastic tissues. When epithelial fluorescence and stromal fluorescence are considered together, diagnostic accuracy is increased to 96.5 %. ConclusionThe clinical diagnosis of cervical neoplasia by spectroscopic methods is potentially a reliable, fast, and costeffective alternative to the conventional smear test which needs trained personnel for its interpretation. Research is still continuing to obtain a statistically significant cutoff value from in vitro studies and then use them for in vivo study.

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“…Keratin and Elastin molecules of connective tissue emit auto fluorescent light at 430 nm. Both molecules are rare in intervertebral discs and do not contribute to disc auto florescence [2,5,8,9] All collagen molecules in all tissues emit auto fluorescent light under UV irradiation [7,13,20,25]. Other molecules-particularly hemoglobin-extinct the auto fluorescence [12].…”

Section: The Origin Of Auto Fluorescencementioning

confidence: 99%

Auto fluorescence of intervertebral disc tissue: a new diagnostic tool

et al. 2006

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The paper reports on auto fluorescence phenomena of inter-vertebral human discs. It systematically investigates the auto fluorescence effects of ex vivo disc specimen and reports on surgical cases to demonstrate the potential value of the new method. The paper offers biologic explanations of the phenomenon and discusses the potential value of the UV auto fluorescence technique as a diagnostic tool. Intraand postoperative observations are made by a surgical microscope with an integrated UV light source. Quantitative measurements were carried out using a photon counter and a spectrometer ex vivo. The auto fluorescence phenomenon allows the differentiation of traumatized and degenerated disc tissue intraoperatively in some cases, it allows the differentiation of bony and collagen endplate in cervical disc surgery. The source of the auto fluorescent light emission are amino acids of the collagen molecules. The proteoglycan components and the liquid components of the disc do not show relevant auto fluorescence. Emission wavelength of disc material is equivalent to color perception. It differs due to different collagen composition of the intervertebral disc components from yellow-green to blue-green and can be visualized in situ by naked eye.UV-auto fluorescence of inter-vertebral discs is a new clinical tool that has the potential to differentiate disc material from the anatomical surrounding, to distinguish between different fractions of the disc and to give information on the quality and status of the disc material. Since the technology has just emerged, it needs further investigations to quantify the clinical observations reported in this paper.

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Understanding the contributions of NADH and collagen to cervical tissue fluorescence spectra: Modeling, measurements, and implications

Abstract: Monte Carlo techniques provide an important means to investigate the combined contributions of multiple fluorophores to measured emission spectra. The approach will prove increasingly valuable as a more sophisticated understanding of in vivo optical properties is developed.

Cited by 262 publications

References 27 publications

Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

Investigation of fiber‐optic probe designs for optical spectroscopic diagnosis of epithelial pre‐cancers

Fluorescence Spectroscopy: A New Approach in Cervical Cancer

Auto fluorescence of intervertebral disc tissue: a new diagnostic tool

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