Understanding the biosynthesis of human IgMs through a combinatorial expression of mutant subunits that affect different assembly steps
Haruki Hasegawa,
Songyu Wang,
Eddie Kast
et al.
Abstract:Polymeric IgMs are secreted from plasma cells abundantly despite their structural complexity and intricate multimerization steps. To gain new insights into IgM’s assembly mechanics that underwrite the high-level secretion, we characterized the biosynthetic process of a natural human IgM, SAM-6, using a recombinant HEK293 cell system. By creating a series of mutant subunits that differentially disrupt specific sets of inter-chain disulfide bonds, we assessed their effects on various aspects of IgM biosynthesis … Show more
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