Understanding the biology of angiogenesis: Review of the most important molecular mechanisms

Otrock, Zaher K.; Mahfouz, Rami; Makarem, J.; Shamseddine, Ali

doi:10.1016/j.bcmd.2007.04.001

Cited by 325 publications

(272 citation statements)

References 127 publications

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“…Human CD34 + cells and hematopoietic precursors secrete several cytokines and growth factors in an autocrine and/or paracrine manner, such as VEGF, HGF, FGF2, Flt3-L, IL-1, IL-16, TGF1, TGF2 and TPO [18,54]. Some of those mediators are mediated in the wound healing process, such as IGF-1, TGF-β1, TNF-, and IL-1 [17]. Analysis of tissue from all the major organs of the mice did not show distribution of human CB-CD34 + cells or pathological changes beyond the wound area and surrounding skin.…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of wound closure in diabetic mice by ex vivo expanded cord blood CD34+ cells

Chotinantakul¹,

Dechsukhum²,

Dejjuy³

et al. 2013

Cellular and Molecular Biology Letters

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Diabetes can impair wound closure, which can give rise to major clinical problems. Most treatments for wound repair in diabetes remain ineffective. This study aimed to investigate the influence on wound closure of treatments using expanded human cord blood CD34 + cells (CB-CD34 + cells), freshly isolated CB-CD34 + cells and a cytokine cocktail. The test subjects were mice with streptozotocin-induced diabetes. Wounds treated with fresh CB-CD34 + cells showed more rapid repair than mice given the PBS control. Injection of expanded CB-CD34 + cells improved wound closure significantly, whereas the injection of the cytokine cocktail alone did not improve wound repair. The results also demonstrated a significant decrease in epithelial gaps and advanced re-epithelialization over the wound bed area after treatment with either expanded CB-CD34 + cells or freshly isolated cells compared with the control. In addition, treatments with both CB-CD34 + cells and the cytokine cocktail were shown to promote recruitment of CD31 + -endothelial cells in the wounds. Both the CB-CD34 + cell population and the cytokine treatments also enhanced the recruitment of CD68-positive cells in the early stages (day 3) of treatment compared with PBS control, although the degree of this enhancement was found to decline in the later stages (day 9). These results demonstrated that expanded CB-CD34 + cells or freshly isolated CB-CD34 + cells could accelerate wound repair by increasing the recruitment of macrophages and capillaries and the reepithelialization over the wound bed area. Our data suggest an effective role in wound closure for both ex vivo expanded CB-CD34 + cells and freshly isolated cells, and these may serve as therapeutic options for wound treatment for diabetic patients. Wound closure acceleration by expanded CB-CD34 + cells also breaks the insufficient quantity obstacle of stem cells per unit of cord blood and other stem cell sources, which indicates a broader potential for autologous transplantation.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, some cytokines and growth factors produced by HSCs have mediatory roles in wound repair, e.g. IGF-1, TGF-β1, TNF-, and IL-1 [12,17,18]. Thus, HSCs are of interest for cell-based therapy for the treatment of wounds.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of wound closure in diabetic mice by ex vivo expanded cord blood CD34+ cells

Chotinantakul¹,

Dechsukhum²,

Dejjuy³

et al. 2013

Cellular and Molecular Biology Letters

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show abstract

“…In the case of uPA, its receptor expression is, indeed, harmonized on the cell surface by FGF-1, -2 and -4 to increase the efficacy of ECM-degrading mechanism FGF-1, -2 and -4 [153]. Gelatinase A (MMP-2) and gelatinase B (MMP-9) have the potency to carry out angiogenic activities [154]. Their release from the endothelial cells is promoted by FGFs [155,156].…”

Section: Fgfs and Fgfrs: Roles In Angiogenesismentioning

confidence: 99%

Fibroblast Growth Factors and their Emerging Cancer-Related Aspects

Khalid¹,

Javaid²

2016

J Cancer Sci Ther

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“…Endothelial cell adhesion onto the extracellular matrix has a central role in endothelial cell migration and angiogenesis (16,17). To examine the role of IER2 in the cell-matrix adhesion, HUVECs were seeded in collagen type I-coated 96-well plates and incubated at 37˚C for 1 h. As shown in Fig.…”

Section: Ier2 Regulates Huvecs Adhesion Onto the Collagen Type I Matrixmentioning

confidence: 99%

Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion

Zhang

et al. 2015

International Journal of Molecular Medicine

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Abstract. Human immediate early response 2 (IER2) has been characterized as a putative nuclear protein that functions as a transcription factor or transcriptional co-activator in the regulation of cellular responses, and may be involved in the regulation of tumor progression and metastasis. Data from our previous gene expression profile of the human microvascular endothelial cells during capillary morphogenesis showed a significant alteration of IER2 expression, suggesting that IER2 may participate in the regulation of the endothelial cell morphogenesis and angiogenesis. The aim of the present study was to investigate the role of IER2 in cell motility, cell-matrix adhesion and in vitro capillary-like structures formation of the human umbilical vein endothelium cells (HUVECs). IER2 was constitutively expressed in HUVECs, and lentiviral-mediated depletion of IER2 significantly reduced the cell motility, cell-matrix adhesion and capillary-like structures formation of HUVECs. Results also showed that depletion and overexpression of IER2 altered the actin cytoskeleton rearrangement in HUVECs. Furthermore, results from western blot analysis showed that the activity of the focal adhesion kinase (FAK) can be regulated by IER2. These results indicated that IER2 regulates endothelial cell motility, adhesion on collagen type I matrix and the capillary tube formation, as the result of the regulation of the actin cytoskeleton rearrangement presumably via a FAK-dependent mechanism.

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Understanding the biology of angiogenesis: Review of the most important molecular mechanisms

Cited by 325 publications

References 127 publications

Enhancement of wound closure in diabetic mice by ex vivo expanded cord blood CD34+ cells

Enhancement of wound closure in diabetic mice by ex vivo expanded cord blood CD34+ cells

Fibroblast Growth Factors and their Emerging Cancer-Related Aspects

Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion

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