Understanding the Basics of DNA Fingerprinting in Forensic Science

Krishnamurthy, Vasavi; Manoj, Reema; Pagare, SS; Kailasam, Senthilkumar

doi:10.5005/jp-journals-10011-1233

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…This identification process is carried out by comparing DNA sequences that are unique to each individual (Choi et al 2008). DNA fingerprinting is widely used in forensics and paternity identification (Krishnamurthy et al 2011). In addition, DNA fingerprints are also widely used to identify genetic diversity and relationships in plants (Selvakumari et al 2017;Jamil et al 2021), poultry genetic diversity (Farrag et al 2010), livestock (Fadhil et al 2013), even widely used for the identification of fish species (Al-Faisal et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

SUKRI¹,

DEWI²,

PRIMAWATI³

et al. 2022

Biodiversitas

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Abstract. Sukri A, Dewi IN, Primawati SB, Wangiyana IGAS, Muttaqin Z, Winaya A. 2022. Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint. Biodiversitas 23: 4153-4159. Sumbawa horse (Equus caballus) is a genetic resource that must be preserved. Sumbawa horses have a significant economic, social, and cultural role. Information on the genetic status of the Sumbawa horse is needed as a step in developing a long-term conservation strategy. Therefore, this study aims to reveal the genetic diversity of the Sumbawa endemic horse using a microsatellite-based DNA fingerprint. DNA isolation was conducted from horse blood samples. Blood samples were taken from two horse farms with 24 individual horses from two different populations, namely Lalar and Liang,West Sumbawa District, Indonesia. Blood was taken from the jugular vein and collected by a qualified veterinarian from the Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika. A total of 4 microsatellite primers were used in this study, INRA032, HEL09, CA425, and AHT4. This study revealed that the genetic diversity of horses in the Lalar population was higher than Liang. A greater number of alleles reinforces this; higher number and frequency of bands; and the presence of specific bands that indicate unique alleles. This research shows that the Sumbawa horse is unique from other horse breeds in the world. This is evidenced by the lower number of alleles per locus (Na) with a maximum number of two alleles per locus. Sumbawa horses have higher observed heterozygosity (Ho) than expected heterozygosity (He), with a Ho value less than 0.5. Analysis of Molecular Variance result has shown that variation within the population was higher than among the population. This is presumably due to the high gene flow in both horse populations caused by inbreeding. In general, AHT4 primers had the best ability to reveal the genetic diversity of Sumbawa horses with the highest Shannon's information index compared to other markers.

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Section: Introductionmentioning

confidence: 99%

Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

SUKRI¹,

DEWI²,

PRIMAWATI³

et al. 2022

Biodiversitas

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DNA Fingerprinting: A Revolution in the Field of Forensic Sciences

Pawar,

Gosavi,

Palaskar

et al. 2023

jfds

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DNA profiling, commonly called DNA fingerprinting or DNA testing, has a wide range of uses, including assisting in criminal cases and wildlife conservation. The firm tissues of the teeth are resistant to external events such as decomposition, bruise, and incineration. Pulp tissue provides a rich DNA source. The review focuses on the various DNA Collection Procedures, the different techniques employed in DNA isolation and amplification. There are a numerous DNA Profiling or Typing systems such as Mitochondrial DNA (mtDNA) analysis, Restriction Fragment Length Polymorphism, Y chromosome analysis, Single Nucleotide Polymorphism (SNP) typing , Short Tandem Repeat (STR) typing, Gender typing, X-chromosome STR Typing. DNA fingerprinting has been applied to solve many cases. Although present DNA analysis techniques are fairly specialized, several upcoming simplifications may increase their simplicity of use.

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Understanding the Basics of DNA Fingerprinting in Forensic Science

Abstract: The human genome contains approximately 3 billion base pairs (bp) of DNA coding for an estimated 50,000 to 100000 genes.

Cited by 2 publications

References 4 publications

Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

DNA Fingerprinting: A Revolution in the Field of Forensic Sciences

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