Understanding the activity of glucose oxidase after exposure to organic solvents

Dudkaitė, Vygailė; Kairys, Visvaldas; Bagdžiūnas, Gintautas

doi:10.1039/d2tb02605h

Cited by 9 publications

(6 citation statements)

References 44 publications

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“…Therefore, we turned to ultraviolet–visible diffuse reflection spectroscopy (UV–vis DRS) and Fourier transform infrared spectroscopy (FT-IR) to confirm the incorporation of GOx in GOx@ZIF-72 and GOx@ZIF-20. The UV–vis DRS adsorption at 450 nm was the π → π* transition of flavin adenine dinucleotide (FAD, the active center of GOx), which confirmed the presence of GOx . Moreover, the infrared absorption band at 1600–1700 cm –1 , assigned to the typical amide I band of the peptide chain of GOx, appeared in both samples of GOx@ZIF-72 and GOx@ZIF-20, verifying the existence of GOx in these two ZIFs (Figure S18).…”

Section: Resultsmentioning

confidence: 65%

“…The UV−vis DRS adsorption at 450 nm was the π → π* transition of flavin adenine dinucleotide (FAD, the active center of GOx), which confirmed the presence of GOx. 43 Moreover, the infrared absorption band at 1600−1700 cm −1 , assigned to the typical amide I band of the peptide chain of GOx, appeared in both samples of GOx@ZIF-72 and GOx@ZIF-20, verifying the existence of GOx in these two ZIFs (Figure S18). However, we noted that the GOx encapsulated by ZIF-20 and ZIF-72 as well as ZIF-7 tended to be inactive, likely caused by the relatively strong alkaline medium required in these ZIFs that was incompatible with the enzyme (Figure S19a).…”

Section: Resultsmentioning

confidence: 71%

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Structural and Functional Insights into the Biomineralized Zeolite Imidazole Frameworks

Huang,

Tong,

Kou

et al. 2023

ACS Nano

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Section: Resultsmentioning

confidence: 65%

Section: Resultsmentioning

confidence: 71%

Structural and Functional Insights into the Biomineralized Zeolite Imidazole Frameworks

Huang,

Tong,

Kou

et al. 2023

ACS Nano

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“…Given the smaller molecular size of HRP (44 kDa [ 32 ]) compared to GOx (160 kDa [ 33 ]), it is difficult for the latter to diffuse through the buckypapers. Additionally, direct spectrometric analysis of the incubation solution after 5 days revealed no absorbance at 278 nm, a characteristic of the FAD cofactor of GOx [ 34 ], thus confirming the retention of this enzyme.…”

Section: Resultsmentioning

confidence: 99%

A Conductive Microcavity Created by Assembly of Carbon Nanotube Buckypapers for Developing Electrochemically Wired Enzyme Cascades

Jeerapan,

Nedellec,

Cosnier

2024

Nanomaterials

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We describe the creation of a conductive microcavity based on the assembly of two pieces of carbon nanotube buckypaper for the entrapment of two enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx), as well as a redox mediator: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS). The hollow electrode, employing GOx, HRP, and the mediator, as an electrochemical enzyme cascade model, is utilized for glucose sensing at a potential of 50 mV vs. Ag/AgCl. This bienzyme electrode demonstrates the ability to oxidize glucose by GOx and subsequently convert H2O2 to water via the electrical wiring of HRP by ABTS. Different redox mediators (ABTS, potassium hexacyanoferrate (III), and hydroquinone) are tested for HRP wiring, with ABTS being the best candidate for the electroenzymatic reduction of H2O2. To demonstrate the possibility to optimize the enzyme cascade configuration, the enzyme ratio is studied with 1 mg HRP combined with variable amounts of GOx (1–4 mg) and 2 mg GOx combined with variable amounts of HRP (0.5–2 mg). The bienzyme electrode shows continuous operational stability for over a week and an excellent storage stability in phosphate buffer, with a decay of catalytic current by only 29% for 1 mM glucose after 100 days.

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“…However, the dissolution of the initiator in methanol also slightly reduced the monomer conversion, suggesting a trade-off between dispersity and yield. This can be explained by the fact that the activity of enzymes like glucose oxidase can be significantly influenced by the solvent environment [ 33 ]. Enzymes, being proteins, have a specific three-dimensional structure crucial for their activity.…”

Section: Resultsmentioning

confidence: 99%

Eco-Conscious Approach to Thermoresponsive Star-Comb and Mikto-Arm Polymers via Enzymatically Assisted Atom Transfer Radical Polymerization Followed by Ring-Opening Polymerization

Fronczyk,

Mielańczyk,

Klymenko

et al. 2023

Molecules

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This study explores the synthesis, characterization, and application of a heterofunctional initiator derived from 2-hydroxypropyl cyclodextrin (HP-β-CD), having eight bromoester groups and thirteen hydroxyl groups allowing the synthesis of mikto-arm star-shaped polymers. The bromoesterification of HP-β-CD was achieved using α-bromoisobutyryl bromide as the acylation reagent, modifying the cyclodextrin (CD) molecule as confirmed by electrospray ionization mass spectrometry (ESI-MS), nuclear magnetic resonance (NMR), attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy analysis, and differential scanning calorimetry (DSC) thermograms. The initiator’s effectiveness was further demonstrated by obtaining star-comb and mikto-arm polymers via an enzymatically assisted atom transfer radical polymerization (ATRP) method and subsequent ring-opening polymerization (ROP). The ATR polymerization quality and control depended on the type of monomer and was optimized by the way of introducing the initiator into the reaction mixture. In the case of ATRP, high conversion rates for poly(ethylene oxide) methyl ether methacrylate (OEOMA), with molecular weights (Mn) of 500 g/mol and 300 g/mol, were achieved. The molecular weight distribution of the obtained polymers remained in the range of 1.23–1.75. The obtained star-comb polymers were characterized by different arm lengths. Unreacted hydroxyl groups in the core of exemplary star-comb polymers were utilized in the ROP of ε-caprolactone (CL) to obtain a hydrophilic mikto-arm polymer. Cloud point temperature (TCP) values of the synthesized polymers increased with arm length, indicating the polymers’ reduced hydrophobicity and enhanced solvation by water. Atomic force microscopy (AFM) analysis revealed the ability of the star-comb polymers to create fractals. The study elucidates advancements in the synthesis and utilization of hydrophilic sugar-based initiators for enzymatically assisted ATRP in an aqueous solution for obtaining complex star-comb polymers in a controlled manner.

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Understanding the activity of glucose oxidase after exposure to organic solvents

Abstract: In this work, the effects of apolar, chlorinated, and polar organic solvents on the activity and structure of glucose oxidase were theoretically and experimentally investigated.

Cited by 9 publications

References 44 publications

Structural and Functional Insights into the Biomineralized Zeolite Imidazole Frameworks

Structural and Functional Insights into the Biomineralized Zeolite Imidazole Frameworks

A Conductive Microcavity Created by Assembly of Carbon Nanotube Buckypapers for Developing Electrochemically Wired Enzyme Cascades

Eco-Conscious Approach to Thermoresponsive Star-Comb and Mikto-Arm Polymers via Enzymatically Assisted Atom Transfer Radical Polymerization Followed by Ring-Opening Polymerization

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