Understanding spatiotemporal mechanical behavior, viscoelasticity, and functions of stem cell-derived cardiomyocytes

Lou, Lihua; Rubfiaro, Alberto Seseña; He, Jin; Agarwal, Arvind

doi:10.1039/d3nr01553j

Cited by 3 publications

(1 citation statement)

References 44 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the diastole phase of S-PLGA-A was 2.5-fold higher than that of S-PLGA-R. The readjustment of the systole–diastole phase is compatible with the pressure–volume loop relationship, where the adult ventricular neat pressure change of isovolumetric relaxation is lower than that of contraction. It further proved that fiber alignment modulates hiPSC-CM maturity and transitions from fetal to adult phenotypes.…”

Section: Resultsmentioning

confidence: 99%

Harnessing 3D Printing and Electrospinning for Multiscale Hybrid Patches Mimicking the Native Myocardium

Lou,

Rubfiaro,

Deng

et al. 2024

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Engineered cardiac tissues show potential for regenerative therapy in ischemic heart disease. Yet, selection of soft biomaterials for scaffold manufacturing is primarily influenced by empirical and compositional factors, raising concerns about arrhythmic risks due to poor electrophysiological integration. Addressing this, we developed multiscale hybrid myocardial patches mimicking native myocardium's structural and biomechanical attributes, utilizing 3D printing and electrospinning techniques. We compared three patch types: pure silicone and siliconepoly(lactic-co-glycolic acid) (PLGA) with random (S-PLGA-R) and aligned (S-PLGA-A) fibers. S-PLGA-A patches with fiber orientation angles of 95−115°are achieved by applying a secondary electrical field using two parallel aluminum enhancers. With bulk and localized moduli of 350−750 and 13−20 kPa resembling the native myocardium, S-PLGA-A patches demonstrate a sarcomere length of 2.1 ± 0.2 μm, ≥50% higher strain motions and diastolic phase, and a 50−70% slower rise of calcium handling compared to the other two patches. This enhanced maturation and improved synchronization phenomena are attributed to efficient force transmission and reduced stress concentration due to mechanical similarity and linear propagation of electrical signals. This study presents a promising strategy for advancing regenerative cardiac therapies by harnessing the capabilities of 3D printing and electrospinning, providing a proof-of-concept for their effectiveness.

show abstract

Section: Resultsmentioning

confidence: 99%

Harnessing 3D Printing and Electrospinning for Multiscale Hybrid Patches Mimicking the Native Myocardium

Lou,

Rubfiaro,

Deng

et al. 2024

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

Multiscale mechanics of polydimethylsiloxane: A comparison of meso‐ and micro‐cyclic deformation behavior

Lou,

Bacca,

et al. 2024

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Despite plenty of static and dynamic mechanical measurements and modeling for bulk polydimethylsiloxane (PDMS) specimens, a notable gap exists in comprehensively understanding the dynamic mechanics under large cycle, low strain conditions, especially for microscale samples. This study integrates tensile testing and nanoindentation techniques to compare dynamic mechanical response for bulk PDMS samples and μ‐pillars. The results from cyclic tensile testing, which involved up to 10,000 cycles at a strain range of 10%–20%, indicate a stabilization of energy dissipation rate after the initial 25 cycles. This attributes to stress relaxation and strain hardening, validating by rapid dual‐phase exponential decay in maximum stress, coupled with an incremental increase in elastic modulus. In comparison to tensile testing, μ‐pillars exhibited a 0.82% reduction in stiffness, stabilizing ~600th cycle. Concurrently, there was an approximately twofold increase in approaching distance during the initial 120 cycles, and an approximately fourfold increase in dissipated energy over the first 80 cycles, before reaching a plateau. This lagging hysteresis effect attributes to the distribution of the resultant force, including top tension, bottom compression, and base tilt. Overall, this study illuminates temporal mechanical deformations in PDMS under two application scenarios, enhancing our understanding of PDMS mechanical behavior.

show abstract

Engineering the Physical Microenvironment into Neural Organoids for Neurogenesis and Neurodevelopment

Li,

Sun,

Hou

et al. 2023

Small

View full text Add to dashboard Cite

Understanding the signals from the physical microenvironment is critical for deciphering the processes of neurogenesis and neurodevelopment. The discovery of how surrounding physical signals shape human developing neurons is hindered by the bottleneck of conventional cell culture and animal models. Notwithstanding neural organoids provide a promising platform for recapitulating human neurogenesis and neurodevelopment, building neuronal physical microenvironment that accurately mimics the native neurophysical features is largely ignored in current organoid technologies. Here, it is discussed how the physical microenvironment modulates critical events during the periods of neurogenesis and neurodevelopment, such as neural stem cell fates, neural tube closure, neuronal migration, axonal guidance, optic cup formation, and cortical folding. Although animal models are widely used to investigate the impacts of physical factors on neurodevelopment and neuropathy, the important roles of human stem cell‐derived neural organoids in this field are particularly highlighted. Considering the great promise of human organoids, building neural organoid microenvironments with mechanical forces, electrophysiological microsystems, and light manipulation will help to fully understand the physical cues in neurodevelopmental processes. Neural organoids combined with cutting‐edge techniques, such as advanced atomic force microscopes, microrobots, and structural color biomaterials might promote the development of neural organoid‐based research and neuroscience.

show abstract

Understanding spatiotemporal mechanical behavior, viscoelasticity, and functions of stem cell-derived cardiomyocytes

Abstract: Understanding myocytes' spatiotemporal mechanical behavior and viscoelasticity is a long-standing challenge as it plays a critical role in regulating structural and functional homeostasis. To probe the time-dependent viscoelastic behaviors of...

Cited by 3 publications

References 44 publications

Harnessing 3D Printing and Electrospinning for Multiscale Hybrid Patches Mimicking the Native Myocardium

Harnessing 3D Printing and Electrospinning for Multiscale Hybrid Patches Mimicking the Native Myocardium

Multiscale mechanics of polydimethylsiloxane: A comparison of meso‐ and micro‐cyclic deformation behavior

Engineering the Physical Microenvironment into Neural Organoids for Neurogenesis and Neurodevelopment

Contact Info

Product

Resources

About