Understanding rice plant resistance to the Brown Planthopper (Nilaparvata lugens): A proteomic approach

Abstract

Engineering and breeding resistant plant varieties are the most effective and environmentally friendly ways to control agricultural pests and improve crop performance. However, the mechanism of plant resistance to pests is poorly understood. Here we used a quantitative mass-spectrometry-based proteomic approach for comparative analysis of expression profiles of proteins in rice leaf sheaths in responses to infestation by the brown planthopper (Nilaparvata lugens Stål, BPH), which is a serious rice crop pest. Proteins involved in multiple pathways showed significant changes in expression in response to BPH feeding, including jasmonic acid synthesis proteins, oxidative stress response proteins, beta-glucanases, protein; kinases, clathrin protein, glycine cleavage system protein, photosynthesis proteins and aquaporins. The corresponding genes of eight important proteins were further analyzed by quantitative RT-PCR. Proteomic and transcript responses that were related to wounding, oxidative and pathogen stress overlapped considerably between BPH-resistant (carrying the resistance gene BPH15) and susceptible rice lines. In contrast, proteins and genes related to callose metabolism remained unchanged and glycine cleavage system protein was up-regulated in the BPH-resistant lines, indicating that they have an efficient and specific defense mechanism. Our results provide new information about the interaction between rice and the BPH.

Inducible Maize Defense Mechanisms Against the Corn Borer Sesamia nonagrioides: A Transcriptome and Biochemical Approach

“…A comprehensive analysis of the transcriptome response in Nicotiana attenuata, a wild relative of tobacco, identified several gene families as being activated in response to insect-inflicted damage, including several transcriptional regulators and protein kinases (Gilardoni et al, 2010; Hermsmeier et al, 2001). Similar results were observed in other plant species (Reymond et al, 2004;Wei et al, 2009). Plant responses to defoliators have been broadly characterized in a number of different plant species (e.g.…”

A deletion in the nitrate high affinity transporter NRT2.1 alters metabolomic and transcriptomic responses to Pseudomonas syringae

“…10 Recent findings in, proteomic analysis of rice have demonstrated that several ribosomal proteins are upregulated after a Nilaparvata lugens infection. 11 Interestingly, the L10 ribosomal protein is upregulated in nrt2 after P. syringae infection compared with Ws. This protein has been shown to be a component in the nuclear shuttle protein-interacting kinase of Arabidopsis, that participates in METLIN (metlin.scripps.edu/).…”

Alteration of plant primary metabolism in response to insect herbivory

“…In most cases, the latter hypothesis, reduced photosynthesis in response to herbivory, is supported by actual measurements of changes in photosynthesis rate, photosynthesis-related gene expression, or production of proteins that are part of the photosynthetic apparatus (Reymond et al, 2004;Giri et al, 2006;Wei et al, 2009;Bilgin et al, 2010;Coppola et al, 2013;Appel et al, 2014). Reduced photosynthetic capacity can exceed what would be expected from the observed amount of foliar tissue that is removed (Zangerl et al, 2002).…”