Understanding prostate cancer biology using metabolomics and proteomics approaches: potentials in the improvement of the diagnosis, prognosis and identification of new therapeutic targets

Felgueiras, Juliana; Silva, Joana Vieira; Nunes, Ashlee A. K.; Patrício, António; Pelech, Steven; Fardilha, Margarida

doi:10.1016/s0959-8049(17)30691-3

Cited by 3 publications

(4 citation statements)

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“…A shift to a lower frequency might suggest alterations in the lipid composition between normal and tumor cells, and an increase in disorder of the CH2 chains of membrane lipids have been associated with malignancy (17). Alterations in the membrane lipids have already been reported in prostate cancer tissue biopsies (21) and breast cancer-derived cell lines (37). Furthermore, alterations regarding lipid saturation have been detected in this spectral region.…”

Section: Discussionmentioning

confidence: 63%

“…The bands at 1152 and 1022 cm -1 are related to carbohydrates, particularly glycogen (which is specifically assigned to the frequency 1022 cm -1 ). A reduced glycogen content has been observed in PCa tissue biopsies (21), and cervical adenocarcinoma tissue and cervical adenocarcinoma cells (38). The decrease in glycogen content in malignant cells can be attributed to their higher metabolic activity and, thus, an increase in glycolysis (11,17,38,56).…”

Section: Discussionmentioning

confidence: 99%

“…Regarding PCa, FTIR spectroscopy has been applied to tissue biopsies and cell lines, showing promising results and elucidating on biochemical alterations inherent to the disease (11,(20)(21)(22). Cell lines present certain advantages that validate their use in preliminary studies, such as the fact that they exhibit a defined cell state that allows the analysis of a target metabolic status.…”

Section: Introductionmentioning

confidence: 99%

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Understanding Prostate Cancer Cells Metabolome: A Spectroscopic Approach

Santos

Magalhães

Henriques

et al. 2019

CMB

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Prostate cancer (PCa) is the second most common neoplasia in men. Because it is often diagnosed at a late stage, mortality rates remain high. Studying cancer metabolome, which reflects early changes that occur in cells, has gained relevance and may contribute to the identification of early diagnostic biomarkers and understanding tumor biology. Fourier-transform infrared (FTIR) spectroscopy is a metabolomics technique that probes the biochemical composition of the analyzed samples and allows to discriminate samples with distinct metabolic profiles, allowing the discrimination between cancerous and non-cancerous samples. In this study, FTIR spectra were acquired from PCa and normal prostate cell lines and analyzed by principal component analysis (PCA). Our results indicate a clear discrimination between the different cell lines, meaning that they exhibit distinct metabolic profiles. This discrimination can be attributed to an altered lipid metabolism (3000-2800 cm -1 , 1800-1700 cm -1 and 1500-1400 cm -1 ) and changes in protein conformation (1700-1600 cm -1 ). These results suggest that studying cancer metabolome with FTIR spectroscopy not only allows the understanding of tumor metabolic behavior and may be useful to the development of new therapeutic targets.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Understanding Prostate Cancer Cells Metabolome: A Spectroscopic Approach

Santos

Magalhães

Henriques

et al. 2019

CMB

View full text Add to dashboard Cite

show abstract

“…Some research groups have already shown promising results in which the high sensitivity of this spectroscopic method was detected in the identification and discrimination of various types of cancer at the level of the spectroscopic analysis of tissue samples from organs such as the lungs [85], breast [86] and prostate [87,88]. In fluid analysis, FTIR has been used in the study of the metabolome associated with physiological alterations, revealing a huge potential in the early and minimally invasive diagnosis of several pathologies [89,90].…”

Section: Ftir Spectroscopy and Thyroid Cancermentioning

confidence: 99%

Metabolic Profile Characterization of Different Thyroid Nodules Using FTIR Spectroscopy: A Review

et al. 2022

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Thyroid cancer’s incidence has increased in the last decades, and its diagnosis can be a challenge. Further and complementary testing based in biochemical alterations may be important to correctly identify thyroid cancer and prevent unnecessary surgery. Fourier-transform infrared (FTIR) spectroscopy is a metabolomic technique that has already shown promising results in cancer metabolome analysis of neoplastic thyroid tissue, in the identification and classification of prostate tumor tissues and of breast carcinoma, among others. This work aims to gather and discuss published information on the ability of FTIR spectroscopy to be used in metabolomic studies of the thyroid, including discriminating between benign and malignant thyroid samples and grading and classifying different types of thyroid tumors.

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8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells

Wang

Chen

Zhang

et al. 2018

Sci Rep

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8u, an acridine derivative, has been proved effective anti-hepatocarcinoma effect, while the underlying mechanism remains unclear. Here, metabolomics and proteomics approaches were applied to study its anti-cancer mechanism and explore its effect on HepG2 cells’ invasion and metastasis abilities. The results showed that 8u significantly suppressed HepG2 cells migration and enhanced cell-to-cell junctions. The inhibition effect of 8u on invasion and metastasis disappeared after HSP90α gene silencing, and was reversed after HSP90α overexpression. The biological experimental results indicated that 8u also blocked PI3K/Akt pathway, thereby reducing fatty acid synthase (FASN) protein expression and disordering intracellular lipid metabolism to inhibit cell invasion and metastasis. In addition, HSP90α protein and PI3K/Akt pathway could co-adjust to each other. These findings demonstrated that 8u could efficiently suppress the invasion and metastasis of HepG2 cells by decreasing the expression of HSP90α protein and inhibiting the PI3K/Akt signaling pathway, which could be used as a potential candidate for the treatment of HCC.

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Understanding prostate cancer biology using metabolomics and proteomics approaches: potentials in the improvement of the diagnosis, prognosis and identification of new therapeutic targets

Cited by 3 publications

References 0 publications

Understanding Prostate Cancer Cells Metabolome: A Spectroscopic Approach

Understanding Prostate Cancer Cells Metabolome: A Spectroscopic Approach

Metabolic Profile Characterization of Different Thyroid Nodules Using FTIR Spectroscopy: A Review

8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells

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