Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System

Bednarek, Piotr Tomasz; Pachota, Katarzyna A.; Dynkowska, Wioletta M.; Machczyńska, Joanna; Orłowska, Renata

doi:10.3390/ijms22147546

Cited by 11 publications

(4 citation statements)

References 232 publications

(266 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was also shown that there are differences in sequence and methylation patterns within symmetric and asymmetric contexts, possibly indicating the role of methylation in green plant regeneration efficiency (GPRE) ( Bednarek et al, 2021a ). However, studies of TCIV based on sequence and DNA methylation changes indicated the phenomenon’s complexity but could not explain its nature ( Bednarek and Orłowska, 2020b ; Ghosh et al, 2021 ; Bednarek et al, 2021b ). At least to some degree, such the opportunity may be born using Fourier Transform Infrared (FTIR) spectroscopy, which provides signals of relevant functional groups and thus allows the identification of chemical compounds in a sample.…”

Section: Introductionmentioning

confidence: 99%

Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticale in vitro anther culture

et al. 2022

Self Cite

View full text Add to dashboard Cite

Plant tissue culture techniques are handy tools for obtaining unique plant materials that are difficult to propagate or important for agriculture. Homozygous materials derived through in vitro cultures are invaluable and significantly accelerate the evaluation of new varieties, e.g., cereals. The induction of somatic embryogenesis/androgenesis and the regeneration and its efficiency can be influenced by the external conditions of tissue culture, such as the ingredients present in the induction or regeneration media. We have developed an approach based on biological system, molecular markers, Fourier Transform Infrared spectroscopy, and structural equation modeling technique to establish links between changes in sequence and DNA methylation at specific symmetric (CG, CHG) and asymmetric (CHH) sequences, glutathione, and green plant regeneration efficiency in the presence of variable supplementation of induction medium with copper ions. The methylation-sensitive Amplified Fragment Length Polymorphism was used to assess tissue culture-induced variation, Fourier Transform Infrared spectroscopy to describe the glutathione spectrum, and a structural equation model to develop the relationship between sequence variation, de novo DNA methylation within asymmetric sequence contexts, and copper ions in the induction medium, as well as, glutathione, and green plant efficiency. An essential aspect of the study is demonstrating the contribution of glutathione to green plant regeneration efficiency and indicating the critical role of copper ions in influencing tissue culture-induced variation, glutathione, and obtaining green regenerants. The model presented here also has practical implications, showing that manipulating the concentration of copper ions in the induction medium may influence cell function and increases green plant regeneration efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticale in vitro anther culture

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Gene expression and phenotypic traits might be affected by chromosome structural variation [ 40 , 41 ]. Microspore culture provides an efficient way to produce homozygosity-stabilized lines with mutation introduction such as DNA degradation and DNA sequence changes [ 42 , 43 ]. 4942C-5 with dwarf and compact plant architecture is a double haploid line obtained from microspore culture.…”

Section: Discussionmentioning

confidence: 99%

Combined BSA-Seq Based Mapping and RNA-Seq Profiling Reveal Candidate Genes Associated with Plant Architecture in Brassica napus

Yan

et al. 2022

IJMS

View full text Add to dashboard Cite

Plant architecture involves important agronomic traits affecting crop yield, resistance to lodging, and fitness for mechanical harvesting in Brassica napus. Breeding high-yield varieties with plant architecture suitable for mechanical harvesting is the main goal of rapeseed breeders. Here, we report an accession of B. napus (4942C-5), which has a dwarf and compact plant architecture in contrast to cultivated varieties. A BC8 population was constructed by crossing a normal plant architecture line, 8008, with the recurrent parent 4942C-5. To investigate the molecular mechanisms underlying plant architecture, we performed phytohormone profiling, bulk segregant analysis sequencing (BSA-Seq), and RNA sequencing (RNA-Seq) in BC8 plants with contrasting plant architecture. Genetic analysis indicated the plant architecture traits of 4942C-5 were recessive traits controlled by multiple genes. The content of auxin (IAA), gibberellin (GA), and abscisic acid (ABA) differed significantly between plants with contrasting plant architecture in the BC8 population. Based on BSA-Seq analysis, we identified five candidate intervals on chromosome A01, namely those of 0 to 6.33 Mb, 6.45 to 6.48 Mb, 6.51 to 6.53 Mb, 6.77 to 6.79 Mb, and 7 to 7.01 Mb regions. The RNA-Seq analysis revealed a total of 4378 differentially expressed genes (DEGs), of which 2801 were up-regulated and 1577 were down-regulated. There, further analysis showed that genes involved in plant hormone biosynthesis and signal transduction, cell structure, and the phenylpropanoid pathway might play a pivotal role in the morphogenesis of plant architecture. Association analysis of BSA-Seq and RNA-Seq suggested that seven DEGs involved in plant hormone signal transduction and a WUSCHEL-related homeobox (WOX) gene (BnaA01g01910D) might be candidate genes responsible for the dwarf and compact phenotype in 4942C-5. These findings provide a foundation for elucidating the mechanisms underlying rapeseed plant architecture and should contribute to breed new varieties suitable for mechanization.

show abstract

“…It was also suggested that methylation may participate in GPRE [ 22 ]. Unfortunately, studies of TCIV failed to explain the nature of the phenomenon (for more information on this, see the reviews [ 23 , 24 ]). Therefore, it was hypothesized that it might have a biochemical background.…”

Section: Introductionmentioning

confidence: 99%

An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants

Orłowska,

Zimny,

Zebrowski

et al. 2024

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

Background The development of the plant in vitro techniques has brought about the variation identified in regenerants known as somaclonal or tissue culture-induced variation (TCIV). S-adenosyl-L-methionine (SAM), glutathione (GSH), low methylated pectins (LMP), and Cu(II) ions may be implicated in green plant regeneration efficiency (GPRE) and TCIV, according to studies in barley (Hordeum vulgare L.) and partially in triticale (× Triticosecale spp. Wittmack ex A. Camus 1927). Using structural equation models (SEM), these metabolites have been connected to the metabolic pathways (Krebs and Yang cycles, glycolysis, transsulfuration), but not for triticale. Using metabolomic and (epi)genetic data, the study sought to develop a triticale regeneration efficiency statistical model. The culture’s induction medium was supplemented with various quantities of Cu(II) and Ag(I) ions for regeneration. The period of plant regeneration has also changed. The donor plant, anther-derived regenerants, and metAFLP were utilized to analyze TCIV concerning DNA in symmetric (CG, CHG) and asymmetric (CHH) sequence contexts. Attenuated Total Reflectance–Fourier Transfer Infrared (ATR-FTIR) spectroscopy was used to gather the metabolomic information on LMP, SAM, and GSH. To frame the data, a structural equation model was employed. Results According to metAFLP analysis, the average sequence change in the CHH context was 8.65%, and 0.58% was de novo methylation. Absorbances of FTIR spectra in regions specific for LMP, SAM, and GSH were used as variables values introduced to the SEM model. The average number of green regenerants per 100 plated anthers was 2.55. Conclusions The amounts of pectin demethylation, SAM, de novo methylation, and GSH are connected in the model to explain GPRE. By altering the concentration of Cu(II) ions in the medium, which influences the amount of pectin, triticale’s GPRE can be increased.

show abstract

Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System

Cited by 11 publications

References 232 publications

Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticale in vitro anther culture

Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticale in vitro anther culture

Combined BSA-Seq Based Mapping and RNA-Seq Profiling Reveal Candidate Genes Associated with Plant Architecture in Brassica napus

An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants

Contact Info

Product

Resources

About