Our first aim was to identify and quantify Biological Volatile Organic Compound (BVOC) emissions, specifically emissions of isoprene, from the moss Polytrichum juniperinum during its earliest stage of life. Isoprene emission from mosses could be a significant component of the total global budget of BVOC emissions. Data concerning the spatial and temporal variability of these emissions are lacking due to poor characterization of the physical and biological factors controlling isoprene synthesis in both vascular and non-vascular plants. We found that P. juniperinum in its early life stage (protonema) can emit isoprene at detectable levels at day five after spore germination. These results suggest that protonema is capable of isoprene emission, shown here for the first time, and media affects emission rates. We saw a negative trend with leaf emergence and protonema growth. Chlorophyll fluorescence ratio had a positive correlation with isoprene emission, and isoprene emission was both temperature and light dependent in early protonema. Our second aim was to determine the effects of sex and sexual expression on isoprene emission in mosses. Sex is a biologically costly, but it can result in genetic adaptability and variety for resulting offspring. Using three Polytrichaceae species, we measured isoprene emission between plants sexually expressing and non-expressing and between male and female moss plants. We found that non-sexually expressing gametophytes had significantly higher isoprene emission than gametophytes expressing sex, suggesting that sex expression may be costly and that additional resources are v Table of Contents