Understanding immunological origins of atopic dermatitis through multi‐omic analysis

Beheshti, Ramin; Halstead, Scott B.; McKeone, Daniel; Hicks, Steven D.

doi:10.1111/pai.13817

Cited by 8 publications

(5 citation statements)

References 34 publications

(90 reference statements)

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“…Patients with AD exhibit thick and darkened skin due to the recurrence and continuation of severe itching, leading to redness, rash formation on wounds, and the development of scabs [ 30 ]. Although the exact etiology remains unclear, AD is a multifactorial skin condition influenced by genetic factors, immunological abnormalities, and environmental changes that interact in a complex manner [ 31 ]. The onset is predominately during infancy [ 32 ], with spontaneous resolution typically occurring by the age of seven; however, some cases may persist into adulthood [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Vinpocetine, a phosphodiesterase 1 inhibitor, mitigates atopic dermatitis-like skin inflammation

Lee,

Song,

Lee

et al. 2024

Korean J Physiol Pharmacol

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Section: Discussionmentioning

confidence: 99%

Vinpocetine, a phosphodiesterase 1 inhibitor, mitigates atopic dermatitis-like skin inflammation

Lee,

Song,

Lee

et al. 2024

Korean J Physiol Pharmacol

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“…RNA was extracted from each sample using the miRNeasy Kit (Qiagen, Inc., Germantown, MD, USA), and RNA quality was assessed with an Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). Downstream RNA processing was performed as we have previously described [ 50 ]. Briefly, RNA sequencing was completed at the SUNY Molecular Analysis Core using the Illumina TruSeq Small RNA Prep protocol and a NextSeq500 instrument (Illumina; San Diego, CA, USA) at a targeted depth of ten million, 50 base, paired-end reads per sample.…”

Section: Methodsmentioning

confidence: 99%

Multi-Omic Factors Associated with Frequency of Upper Respiratory Infections in Developing Infants

Beheshti

Halstead

Cusack

et al. 2023

IJMS

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Susceptibility to upper respiratory infections (URIs) may be influenced by host, microbial, and environmental factors. We hypothesized that multi-omic analyses of molecular factors in infant saliva would identify complex host-environment interactions associated with URI frequency. A cohort study involving 146 infants was used to assess URI frequency in the first year of life. Saliva was collected at 6 months for high-throughput multi-omic measurement of cytokines, microRNAs, transcripts, and microbial RNA. Regression analysis identified environmental (daycare attendance, atmospheric pollution, breastfeeding duration), microbial (Verrucomicrobia, Streptococcus phage), and host factors (miR-22-5p) associated with URI frequency (p < 0.05). These results provide pathophysiologic clues about molecular factors that influence URI susceptibility. Validation of these findings in a larger cohort could one day yield novel approaches to detecting and managing URI susceptibility in infants.

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“…Samples were aliquoted and stored at −80 °C within two weeks of sample collection, per manufacturer instructions. As we have previously reported, RNA was extracted from each sample using the miRNeasy Kit (Qiagen, Inc., Germantown, MD, USA), and RNA quality was assessed with an Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) [ 53 , 54 , 55 ]. RNA sequencing was completed at the SUNY Molecular Analysis Core using the Illumina TruSeq Small RNA Prep protocol and a NextSeq500 instrument (Illumina; San Diego, CA, USA) at a targeted depth of ten million, 50-base, paired-end reads per sample.…”

Section: Methodsmentioning

confidence: 99%

Infant Saliva Levels of microRNA miR-151a-3p Are Associated with Risk for Neurodevelopmental Delay

Hicks

Confair

2023

IJMS

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Prompt recognition of neurodevelopmental delay is critical for optimizing developmental trajectories. Currently, this is achieved with caregiver questionnaires whose sensitivity and specificity can be limited by socioeconomic and cultural factors. This prospective study of 121 term infants tested the hypothesis that microRNA measurement could aid early recognition of infants at risk for neurodevelopmental delay. Levels of four salivary microRNAs implicated in childhood autism (miR-125a-5p, miR-148a-5p, miR-151a-3p, miR-28-3p) were measured at 6 months of age, and compared between infants who displayed risk for neurodevelopmental delay at 18 months (n = 20) and peers with typical development (n = 101), based on clinical evaluation aided by the Survey of Wellbeing in Young Children (SWYC). Accuracy of microRNAs for predicting neurodevelopmental concerns at 18 months was compared to the clinical standard (9-month SWYC). Infants with neurodevelopmental concerns at 18 months displayed higher levels of miR-125a-5p (d = 0.30, p = 0.018, adj p = 0.049), miR-151a-3p (d = 0.30, p = 0.017, adj p = 0.048), and miR-28-3p (d = 0.31, p = 0.014, adj p = 0.048). Levels of miR-151a-3p were associated with an 18-month SWYC score (R = −0.19, p = 0.021) and probability of neurodevelopmental delay at 18 months (OR = 1.91, 95% CI, 1.14–3.19). Salivary levels of miR-151a-3p enhanced predictive accuracy for future neurodevelopmental delay (p = 0.010, X2 = 6.71, AUC = 0.71) compared to the 9-month SWYC score alone (OR = 0.56, 95% CI, 0.20–1.58, AUC = 0.567). This pilot study provides evidence that miR-151a-3p may aid the identification of infants at risk for neurodevelopmental delay. External validation of these findings is necessary.

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Understanding immunological origins of atopic dermatitis through multi‐omic analysis

Cited by 8 publications

References 34 publications

Vinpocetine, a phosphodiesterase 1 inhibitor, mitigates atopic dermatitis-like skin inflammation

Vinpocetine, a phosphodiesterase 1 inhibitor, mitigates atopic dermatitis-like skin inflammation

Multi-Omic Factors Associated with Frequency of Upper Respiratory Infections in Developing Infants

Infant Saliva Levels of microRNA miR-151a-3p Are Associated with Risk for Neurodevelopmental Delay

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