Understanding how the substituents of polysaccharides influence physical properties

Aggarwal, Shalu; Koser, Kulsoom; Chakravarty, Archana; Ikram, Saiqa

doi:10.1016/b978-0-12-823439-6.00009-x

Cited by 1 publication

(1 citation statement)

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“…Mushrooms are naturally capable of accumulating minerals and vitamins, as well as a variety of secondary metabolites including organic acids, alkaloids, phenols, and terpenoids 42 . Mushroom polysaccharides differ from other types of polysaccharides in regards to molecular weights, chemical characteristics, degree of branching, skeleton lengths, three-dimensional orientation, and other properties 43 . The administration of PsPc-3 www.nature.com/scientificreports/ extract significantly modifies the dysfunction of nearby renal tubular cells by slightly restoring the indicators of renal function in the blood to their normal limits and restoring the activity of the antioxidant system.…”

Section: Discussionmentioning

confidence: 95%

Evaluating the role of polysaccharide extracted from Pleurotus columbinus on cisplatin-induced oxidative renal injury

Mohamed

Ibrahim

Ghoneim

et al. 2023

Sci Rep

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This research aimed to examine the antioxidant polysaccharide activity (PsPc-3) derived from Pleurotus columbinus (P. columbinus) on oxidative renal injury (ORI) induced by cisplatin (CP). The principal components of crude polysaccharide were assessed. We studied the preventive impact of polysaccharide on cisplatin-induced renal damage in this study. For 21 days, we employed the CP-induced ORI rat model and divided the rats into four groups: control, CP alone, polysaccharide post CP (100 mg/kg) orally, and CP + polysaccharide (pre and post). The chemical characterization of the polysaccharide fraction PsPc-3 stated that protein was not present. PsPc-3 contained 7.2% uronic acid as assessed as 0% sulfate. PsPc-3 hydrolysate structured of Galacturonic:Glucose:Xylose and their molar proportions were 1:4:5, respectively. The average molecular weight (Mw) and molecular mass (Mn) per molecule of PsPc-3 were 5.49 × 104 g/mol and Mn of 4.95 × 104 g/mol respectively. DPPH radical scavenging activity was demonstrated by the polysaccharide of 65.21–95.51% at 10 mg/ml with IC50 less than 10 mg/ml. CP increased serum urea to 92.0 mg/dl and creatinine up to 1.0 mg/dl, with a concurrent decrease in the levels of total protein to 4.0 mg/dl. Besides, Also, CP-induced ORI raised levels of malondialdehyde (MDA), alkaline phosphatase (ALP), and renal hormones (renin and aldosterone), with a decline in antioxidants compared to control rats. In addition, in the presence of CP, interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) levels increased. PsPc-3 decreased these changes dramatically. PsPc-3 improves pathological renal damage caused by CP and decreases tubular apoptosis measured by DNA ladder formation and cleaved caspase- 3. These findings showed that PsPc-3 isolated from P. columbinus protects and inhibits tubular apoptosis in cisplatin-induced ORI. Furthermore, PsPc-3 has no influence on the anticancer efficacy of CP in rats. Thus, PsPc-3 derived from P. columbinus might provide a novel therapy method for cisplatin-induced nephrotoxicity.

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Section: Discussionmentioning

confidence: 95%