Understanding genetic diversity, spatial genetic structure, and mating system through microsatellite markers for the conservation and sustainable use of Acrocomia aculeata (Jacq.) Lodd. Ex Mart.

Coelho, Natália Helena Pesso; Tambarussi, Evandro Vagner; Aguiar, Bruna Ibanes; Roque, Rafael Henrique; Portela, Renan Marcelo; Braga, Rayssa Chaves; Sanson, Daniela; Silva, Richeliel A. R.; Ferraz, Elza Martins; Moreno, Marlene Aparecida; Kageyama, Paulo Yoshio; Gandara, Flávio Bertin

doi:10.1007/s10592-018-1061-z

Cited by 22 publications

(34 citation statements)

References 43 publications

(72 reference statements)

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“…In the present study, microsatellite markers of expressed regions (EST-SSR) and genomic regions (gSSR) were adopted to elucidate the taxonomic relationship between the most economically important Acrocomia species (A. aculeata and A. totai ) and to identify possible interspecific hybrids. In the genus Acrocomia, several genetic studies performed with microsatellite markers were conducted with at most 8 gSSR loci developed for A. aculeata by Nucci et al (2008), as in Lanes et al (2015), Lanes et al (2016), Mengistu et al (2016), Neiva et al (2016), Araújo et al (2017), Coelho et al, (2018) and Lima et al (2020). Thus, this work represents the first study using both EST-SSR and gSSR markers in Acrocomia.…”

Section: Genetic Variability In Genomic Gssrs and Est-ssrsmentioning

confidence: 99%

“…Studies on the genetic diversity of accessions of populations in Mato Grosso do Sul (Lanes at al. 2015;Mengsitu 2016) and in the border region between the states of São Paulo and Mato Grosso do Sul (Abreu et al, 2012;Coelho et al, 2018) considered A. aculeata as study material. A single study was reported on the genetic diversity ofA.…”

Section: Inter and Intraspecific Genetic Diversitymentioning

confidence: 99%

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Species delimitation and hybrid identification of Acrocomia aculeata and A. totai by genetic population approach

Diaz

Zucchi

Alves‐Pereira

et al. 2020

Preprint

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To the Neotropical genus Acrocomia (Arecaceae) is attributed eight species with a wide distribution in America. A. aculeata and A. totai are the most important species because of their high economic potential for oil production. However, there is no consensus in their classification as different taxons and their distinctiveness is particularly challenging due to morphological similarities with large plasticity of the traits. In addition, there is doubt about the occurrence of interspecific hybrids between both species. In this study, we applied a genetic population approach to assessing the genetic boundaries, diversity and to identify interspecific hybrids of A. aculeata and A. totai. Thirteen loci of simple sequence repeat (SSR) were employed to analyze twelve populations representing a wide distribution of species, from Minas Gerais, Brazil to Formosa, Argentina. Based on the Bayesian analysis (STRUCTURE and NewHybrids) and Discriminant Analysis of Principal Components (DAPC), our study supports the recognition of A. aculeata and A. totai as two species and the estimates of genetic parameters revealed more genetic diversity in A. totai (H E =0.551) than in A. aculeata (H E =0.466). We obtained evidence of hybridization between the species and that admixed individuals were assigned as F2 hybrids. In conclusion, this study showed the usefulness of microsatellite markers to elucidate the genetic boundaries of A. aculeata and A. totai, supporting their classification as different species and increase our knowledge about genetic diversity at the level of populations and species. The results are essentials to establish strategies for the adequate management, conservation, and domestication of both species.

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Section: Genetic Variability In Genomic Gssrs and Est-ssrsmentioning

confidence: 99%

Section: Inter and Intraspecific Genetic Diversitymentioning

confidence: 99%

Species delimitation and hybrid identification of Acrocomia aculeata and A. totai by genetic population approach

Diaz

Zucchi

Alves‐Pereira

et al. 2020

Preprint

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“…At the intraspecific level, the highest genetic diversity for the species was found in Brazil, especially in the States of Minas Gerais and São Paulo (Table 3). Although it is not possible to make direct comparisons due to the different types of molecular markers used, previous studies also identified a high genetic diversity for A. aculeata in the States of Minas Gerais and São Paulo [23,28,29].…”

Section: A Aculeatamentioning

confidence: 99%

“…Other approaches include the use of internal transcribed ribosomal 18S-26S spacer (ITS region) [27] and random amplification of polymorphic DNA (RAPD) markers [28]. However, most studies have focused on A. aculeata [12,23,25,26,29]. Only one study has analyzed the genetic diversity of A. totai (Lima et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Whole-genome SNP analysis elucidates the genetic population structure and diversity of Acrocomia species

Diaz

Zucchi

Alves‐Pereira

et al. 2020

Preprint

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Acrocomia (Arecaceae) is a genus widely distributed in tropical and subtropical America that has been achieving economic interest due to the great potential of oil production of some of its species. In particular A. aculeata, due to its vocation to supply oil with the same productive capacity as the oil palm even in areas with water deficit. Although eight species are recognized in the genus, the taxonomic classification based on morphology and geographic distribution is still controversial. Knowledge about the genetic diversity and population structure of the species is limited, which has limited the understanding of the genetic relationships and the orientation of management, conservation, and genetic improvement activities of species of the genus. In the present study, we analyzed the genomic diversity and population structure of seven species of Acrocomia including 117 samples of A. aculeata covering a wide geographical area of occurrence, using single nucleotide Polymorphism (SNP) markers originated from Genotyping By Sequencing (GBS). The genetic structure of the Acrocomia species were partially congruent with the current taxonomic classification based on morphological characters, recovering the separation of the species A. aculeata, A. totai, A. crispa and A. intumescens as distinct taxonomic groups. However, the species A. media was attributed to the cluster of A. aculeata while A. hassleri and A. glauscescens were grouped together with A. totai. The species that showed the highest and lowest genetic diversity were A. totai and A. media, respectively. When analyzed separately, the species A. aculeata showed a strong genetic structure, forming two genetic groups, the first represented mainly by genotypes from Brazil and the second by accessions from Central and North American countries. Greater genetic diversity was found in Brazil when compared to the other countries. Our results on the genetic diversity of the genus are unprecedented, as is also establishes new insights on the genomic relationships between Acrocomia species. It is also the first study to provide a more global view of the genomic diversity of A. aculeata. We also highlight the applicability of genomic data as a reference for future studies on genetic diversity, taxonomy, evolution and phylogeny of the Acrocomia genus, as well as to support strategies for the conservation, exploration and breeding of Acrocomia species and in particular A. aculeata.

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“…The marker of choice for many areas of population research, such as mating systems, kinship structure, demography or conservation genetics, are microsatellite loci 6 – 8 . The usefulness of these markers results from their codominance, high polymorphism, and abundance throughout the genome 9 .…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

Kostro‐Ambroziak

Siekiera

Czajkowska

et al. 2020

Sci Rep

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Microsatellite loci are commonly used markers in population genetic studies. In this study, we present 40 novel and polymorphic microsatellite loci elaborated for the ichneumonid parasitoid Latibulus argiolus (Rossi, 1790). Reaction condition optimisation procedures allowed 14 of these loci to be co-amplified in two PCRs and loaded in two multiplex panels onto a genetic analyser. The assay was tested on 197 individuals of L. argiolus originating from ten natural populations obtained from the host nests of paper wasps. The validated loci were polymorphic with high allele numbers ranging from eight to 27 (average 17.6 alleles per locus). Both observed and expected heterozygosity values were high, ranging between 0.75 and 0.92 for HO (mean 0.83) and from 0.70 to 0.90 for HE (mean 0.85). The optimized assay showed low genotyping error rate and negligible null allele frequency. The designed multiplex panels could be successfully applied in relatedness analyses and genetic variability studies of L. argiolus populations, which would be particularly interesting considering the coevolutionary context of this species with its social host.

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Understanding genetic diversity, spatial genetic structure, and mating system through microsatellite markers for the conservation and sustainable use of Acrocomia aculeata (Jacq.) Lodd. Ex Mart.

Cited by 22 publications

References 43 publications

Species delimitation and hybrid identification of Acrocomia aculeata and A. totai by genetic population approach

Species delimitation and hybrid identification of Acrocomia aculeata and A. totai by genetic population approach

Whole-genome SNP analysis elucidates the genetic population structure and diversity of Acrocomia species

Development of microsatellite loci and optimization of a multiplex assay for Latibulus argiolus (Hymenoptera: Ichneumonidae), the specialized parasitoid of paper wasps

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